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Outputs (15)

A range of voltage-clamp protocol designs for rapid capture of hERG kinetics (2024)
Preprint / Working Paper
Lei, C. L., J Whittaker, D., J Windley, M., D Perry, M., P Hill, A., & R Mirams, G. A range of voltage-clamp protocol designs for rapid capture of hERG kinetics

We provide details of a series of short voltage-clamp protocols designed for gathering a large amount of information on hERG (Kv11.1) ion channel gating. The protocols have a limited number of steps and consist only of steps and ramps, making them ea... Read More about A range of voltage-clamp protocol designs for rapid capture of hERG kinetics.

Optimising experimental designs for model selection of ion channel drug binding mechanisms (2024)
Preprint / Working Paper
Patten-Elliott, F., Lei, C. L., Preston, S. P., Wilkinson, R. D., & Mirams, G. R. Optimising experimental designs for model selection of ion channel drug binding mechanisms

The rapid delayed rectifier current carried by the human Ether-à-go-go-Related Gene (hERG) channel is susceptible to drug-induced reduction which can lead to an increased risk of cardiac arrhythmia. Establishing the mechanism by which a specific drug... Read More about Optimising experimental designs for model selection of ion channel drug binding mechanisms.

Evaluating the predictive accuracy of ion channel models using data from multiple experimental designs (2024)
Preprint / Working Paper
Shuttleworth, J. G., Lei, C. L., Windley, M. J., Hill, A. P., Preston, S. P., & Mirams, G. R. Evaluating the predictive accuracy of ion channel models using data from multiple experimental designs

Mathematical models are increasingly being relied upon to provide quantitatively accurate predictions of cardiac electrophysiology. Many such models concern the behaviour of particular subcellular components (namely, ion channels) which, together, al... Read More about Evaluating the predictive accuracy of ion channel models using data from multiple experimental designs.

A range of voltage-clamp protocol designs for rapid capture of hERG kinetics (2024)
Preprint / Working Paper
Lei, C. L., Whittaker, D. G., Windley, M. J., Perry, M. D., Hill, A. P., & Mirams, G. R. A range of voltage-clamp protocol designs for rapid capture of hERG kinetics

We provide details of a series of short voltage-clamp protocols designed for gathering a large amount of information on hERG (Kv11.1) ion channel gating. The protocols have a limited number of steps and consist only of steps and ramps, making them ea... Read More about A range of voltage-clamp protocol designs for rapid capture of hERG kinetics.

Resolving artefacts in voltage-clamp experiments with computational modelling: an application to fast sodium current recordings. (2024)
Preprint / Working Paper
Lei, C. L., Clark, A. P., Clerx, M., Wei, S., Bloothooft, M., de Boer, T. P., Christini, D. J., Krogh-Madsen, T., & Mirams, G. R. Resolving artefacts in voltage-clamp experiments with computational modelling: an application to fast sodium current recordings

Cellular electrophysiology is the foundation of many fields, from basic science in neurology, cardiology, oncology to safety critical applications for drug safety testing, clinical phenotyping, etc. Patch-clamp voltage clamp is the gold standard tech... Read More about Resolving artefacts in voltage-clamp experiments with computational modelling: an application to fast sodium current recordings..

Optimal experimental designs for characterising ion channel gating by filling the phase-voltage space of model dynamics (2024)
Journal Article
Mirams, G. R., Clerx, M., Whittaker, D. G., & Lei, C. L. (2024). Optimal experimental designs for characterising ion channel gating by filling the phase-voltage space of model dynamics. Mathematics in Medical and Life Sciences, 2024(1), Article 2375494. https://doi.org/10.1080/29937574.2024.2375494

Voltage-clamp waveforms are imposed in the patch-clamp electrophysiology technique to provoke ion currents, the particular waveform that is used is known as the “voltage-clamp protocol”. Designing protocols to probe and quantify how gating for a part... Read More about Optimal experimental designs for characterising ion channel gating by filling the phase-voltage space of model dynamics.

Optimal experimental designs for characterising ion channel gating by filling the phase-voltage space of model dynamics (2024)
Journal Article
Mirams, G. R., Clerx, M., Whittaker, D. G., & Lei, C. L. (2024). Optimal experimental designs for characterising ion channel gating by filling the phase-voltage space of model dynamics. Mathematics in Medical and Life Sciences, 1(1), Article 2375494. https://doi.org/10.1080/29937574.2024.2375494

Voltage-clamp waveforms are imposed in the patch-clamp electrophysiology technique to provoke ion currents, the particular waveform that is used is known as the “voltage-clamp protocol”. Designing protocols to probe and quantify how gating for a part... Read More about Optimal experimental designs for characterising ion channel gating by filling the phase-voltage space of model dynamics.

An experimental investigation of rundown of the L-type calcium current [version 1; peer review: awaiting peer review] (2024)
Journal Article
Agrawal, A., Clerx, M., Wang, K., Gissinger, E., Gavaghan, D. J., Polonchuk, L., & Mirams, G. R. (in press). An experimental investigation of rundown of the L-type calcium current [version 1; peer review: awaiting peer review]. Wellcome Open Research, 9, 250. https://doi.org/10.12688/wellcomeopenres.20374.1

Background

L-type calcium channels (LCCs) are multi-protein macro-molecular ion channel complexes that are involved in several critical functions in cardiac, skeletal, neuronal, smooth muscle, and endocrine cells. Like other ion channels, LCCs can... Read More about An experimental investigation of rundown of the L-type calcium current [version 1; peer review: awaiting peer review].