Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs
(2016)
Journal Article
Bertero, A., Pawlowski, M., Ortmann, D., Snijders, K., Yiangou, L., Cardoso de Brito, M., …Vallier, L. (2016). Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs. Development, 143(23), 4405-4418. https://doi.org/10.1242/dev.138081
© 2016. Inducible loss of gene function experiments are necessary to uncover mechanisms underlying development, physiology and disease. However, current methods are complex, lack robustness and do not work in multiple cell types. Here we address thes... Read More about Optimized inducible shRNA and CRISPR/Cas9 platforms for in vitro studies of human development using hPSCs.