Outputs (7)

Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase (2023)
Journal Article
Buckley, R. J., Lou-Hing, A., Hanson, K. M., Ahmed, N. R., Cooper, C. D., & Bolt, E. L. (2023). Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase. Molecular Microbiology, 120(2), 298-306. https://doi.org/10.1111/mmi.15123

DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well-characterized DNA repair helicases that are fused to additional 600–700 amino acids of un... Read More about Escherichia coli DNA repair helicase Lhr is also a uracil-DNA glycosylase.

Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase (2023)
Journal Article
Buckley, R. J., Lou‐Hing, A., Hanson, K. M., Ahmed, N. R., Cooper, C. D. O., & Bolt, E. L. (2023). Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase. Molecular Microbiology, 120(2), 298-306. https://doi.org/10.1111/mmi.15123

DNA glycosylases protect genetic fidelity during DNA replication by removing potentially mutagenic chemically damaged DNA bases. Bacterial Lhr proteins are well‐characterized DNA repair helicases that are fused to additional 600–700 amino acids of un... Read More about Escherichia coli DNA repair helicase Lhr is also a uracil‐DNA glycosylase.

Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing (2023)
Journal Article
Lever, R., Simmons, E., Gamble-Milner, R., Buckley, R., Harrison, C., Parkes, A., …Allers, T. (2023). Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing. Nucleic Acids Research, https://doi.org/10.1093/nar/gkad572

Hel308 helicases promote genome stability in archaea and are conserved in metazoans, where they are known as HELQ. Their helicase mechanism is well characterised, but it is unclear how they specifically contribute to genome stability in archaea. We s... Read More about Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing.

Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation (2023)
Journal Article
Killelea, T., Dimude, J. U., He, L., Stewart, A. L., Kemm, F. E., Radovčí, M., …Bolt, E. L. (2023). Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation. Nucleic Acids Research, 51(13), 6914-6926. https://doi.org/10.1093/nar/gkad473

Prokaryotic Cas1-Cas2 protein complexes generate adaptive immunity to mobile genetic elements (MGEs), by capture and integration of MGE DNA in to CRISPR sites. De novo immunity relies on naive adaptation-Cas1-Cas2 targeting of MGE DNA without the aid... Read More about Cas1-Cas2 physically and functionally interacts with DnaK to modulate CRISPR Adaptation.

Editorial: Structural biology of nucleic acid replication, recombination and repair (2023)
Journal Article
Bolt, E. L., Cooper, C. D., & Robinson, N. P. (2023). Editorial: Structural biology of nucleic acid replication, recombination and repair. Frontiers in Molecular Biosciences, 10, Article 1155089. https://doi.org/10.3389/fmolb.2023.1155089

CRISPR-Cas adaptation in Escherichia coli (2023)
Journal Article
Mitic, D., Bolt, E. L., & Ivancic-Bace, I. (2023). CRISPR-Cas adaptation in Escherichia coli. Bioscience Reports, 43(3), Article BSR20221198. https://doi.org/10.1042/BSR20221198

Prokaryotes use the adaptive immunity mediated via the Clustered Regularly Interspaced Short Palindromic Repeats and CRISPR associated (CRISPR-Cas) system for protection against invading elements such as phages and plasmids. The immunity is achieved... Read More about CRISPR-Cas adaptation in Escherichia coli.

Interaction of human HelQ with DNA polymerase delta halts DNA synthesis and stimulates DNA single-strand annealing (2023)
Journal Article
He, L., Lever, R., Cubbon, A., Tehseen, M., Jenkins, T., Nottingham, A. O., …Bolt, E. L. (2023). Interaction of human HelQ with DNA polymerase delta halts DNA synthesis and stimulates DNA single-strand annealing. Nucleic Acids Research, 51(4), 1740-1749. https://doi.org/10.1093/nar/gkad032

DNA strand breaks are repaired by DNA synthesis from an exposed DNA end paired with a homologous DNA template. DNA polymerase delta (Pol δ) catalyses DNA synthesis in multiple eukaryotic DNA break repair pathways but triggers genome instability unles... Read More about Interaction of human HelQ with DNA polymerase delta halts DNA synthesis and stimulates DNA single-strand annealing.