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GC preps: Fast and easy extraction of stable yeast genomic DNA (2016)
Journal Article
Blount, B. A., Driessen, M. R., & Ellis, T. (2016). GC preps: Fast and easy extraction of stable yeast genomic DNA. Scientific Reports, 6, Article 26863. https://doi.org/10.1038/srep26863

Existing yeast genomic DNA extraction methods are not ideally suited to extensive screening of colonies by PCR, due to being too lengthy, too laborious or yielding poor quality DNA and inconsistent results. We developed the GC prep method as a soluti... Read More about GC preps: Fast and easy extraction of stable yeast genomic DNA.

Wallerian degeneration is executed by an NMN-SARM1-dependent late Ca(2+) influx but only modestly influenced by mitochondria (2015)
Journal Article
Loreto, A., Di Stefano, M., Gering, M., & Conforti, L. (2015). Wallerian degeneration is executed by an NMN-SARM1-dependent late Ca(2+) influx but only modestly influenced by mitochondria. Cell Reports, 13(11), 2539-2552. https://doi.org/10.1016/j.celrep.2015.11.032

Axon injury leads to rapid depletion of NAD-biosynthetic enzyme NMNAT2 and high levels of its substrate, NMN. We proposed a key role for NMN in Wallerian degeneration but downstream events and their relationship to other mediators remain unclear. Her... Read More about Wallerian degeneration is executed by an NMN-SARM1-dependent late Ca(2+) influx but only modestly influenced by mitochondria.

Horizontal gene flow from Eubacteria to Archaebacteria and what it means for our understanding of eukaryogenesis (2015)
Journal Article
Akanni, W. A., Siu-Ting, K., Creevey, C. J., McInerney, J. O., Wilkinson, M., Foster, P. G., & Pisani, D. (2015). Horizontal gene flow from Eubacteria to Archaebacteria and what it means for our understanding of eukaryogenesis. Philosophical Transactions B: Biological Sciences, 370(1678), Article 20140337. https://doi.org/10.1098/rstb.2014.0337

© 2015 The Author(s) Published by the Royal Society. All rights reserved. The origin of the eukaryotic cell is considered one of the major evolutionary transitions in the history of life. Current evidence strongly supports a scenario of eukaryotic or... Read More about Horizontal gene flow from Eubacteria to Archaebacteria and what it means for our understanding of eukaryogenesis.

Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O2 Demonstrate That the Reaction Is Partly Reversible (2015)
Journal Article
Orain, C., Saujet, L., Gauquelin, C., Soucaille, P., Meynial-Salles, I., Baffert, C., Fourmond, V., Bottin, H., & Léger, C. (2015). Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O2 Demonstrate That the Reaction Is Partly Reversible. Journal of the American Chemical Society, 137(39), 12580-12587. https://doi.org/10.1021/jacs.5b06934

© 2015 American Chemical Society. The mechanism of reaction of FeFe hydrogenases with oxygen has been debated. It is complex, apparently very dependent on the details of the protein structure, and difficult to study using conventional kinetic techniq... Read More about Electrochemical Measurements of the Kinetics of Inhibition of Two FeFe Hydrogenases by O2 Demonstrate That the Reaction Is Partly Reversible.

A potential role for cannabinoid receptors in the therapeutic action of fenofibrate (2014)
Journal Article
Priestley, R. S., Nickolls, S. A., Alexander, S. P. H., & Kendall, D. A. (2015). A potential role for cannabinoid receptors in the therapeutic action of fenofibrate. FASEB Journal, 29(4), 1446-1455. https://doi.org/10.1096/fj.14-263053

Cannabinoids are reported to have actions through peroxisome proliferator-activated receptors (PPARs), which led us to investigate PPAR agonists for activity at the cannabinoid receptors. Radio-ligand binding and functional assays were conducted usin... Read More about A potential role for cannabinoid receptors in the therapeutic action of fenofibrate.

In vitroevaluation of biomimetic chitosan–calcium phosphate scaffolds with potential application in bone tissue engineering (2013)
Journal Article
Tanase, C. E., Sartoris, A., Popa, M. I., Verestiuc, L., Unger, R. E., & Kirkpatrick, C. J. (2013). In vitroevaluation of biomimetic chitosan–calcium phosphate scaffolds with potential application in bone tissue engineering. Biomedical Materials, 8(2), Article 025002. https://doi.org/10.1088/1748-6041/8/2/025002

This work reports on the physicochemical properties and in vitro cytotoxicity assessment of chitosan–calcium phosphate (Cs–CP) scaffolds for bone tissue engineering, which were synthesized by a novel biomimetic co-precipitation method. X-ray diffract... Read More about In vitroevaluation of biomimetic chitosan–calcium phosphate scaffolds with potential application in bone tissue engineering.

Methicillin resistance reduces the virulence of healthcare-associated methicillin-resistant staphylococcus aureus by interfering with the agr quorum sensing system (2012)
Journal Article
Rudkin, J. K., Edwards, A. M., Bowden, M. G., Brown, E. L., Pozzi, C., Waters, E. M., Chan, W. C., Williams, P., O'Gara, J. P., & Massey, R. C. (2012). Methicillin resistance reduces the virulence of healthcare-associated methicillin-resistant staphylococcus aureus by interfering with the agr quorum sensing system. Journal of Infectious Diseases, 205(5), 798-806. https://doi.org/10.1093/infdis/jir845

The difficulty in successfully treating infections caused by methicillin-resistant Staphylococcus aureus (MRSA) has led to them being referred to as highly virulent or pathogenic. In our study of one of the major healthcare-associated MRSA (HA-MRSA)... Read More about Methicillin resistance reduces the virulence of healthcare-associated methicillin-resistant staphylococcus aureus by interfering with the agr quorum sensing system.