Stephen Henry
Rapid one-step biotinylation of biological and non-biological surfaces
Henry, Stephen; Williams, Eleanor; Barr, Katie; Korchagina, Elena; Tuzikov, Alexandr; Ilyushina, Natalia; Abayzeed, Sidahmed A.; Webb, Kevin F.; Bovin, Nicolai
Authors
Eleanor Williams
Katie Barr
Elena Korchagina
Alexandr Tuzikov
Natalia Ilyushina
SIDAHMED ABAYZEED SIDAHMED.ABAYZEED2@NOTTINGHAM.AC.UK
Assistant Professor
Dr KEVIN WEBB KEVIN.WEBB@NOTTINGHAM.AC.UK
Associate Professor
Nicolai Bovin
Abstract
We describe a rapid one-step method to biotinylate virtually any biological or non-biological surface. Contacting a solution of biotin-spacer-lipid constructs with a surface will form a coating within seconds on non-biological surfaces or within minutes on most biological membranes including membrane viruses. The resultant biotinylated surface can then be used to interact with avidinylated conjugates, beads, vesicles, surfaces or cells.
Citation
Henry, S., Williams, E., Barr, K., Korchagina, E., Tuzikov, A., Ilyushina, N., …Bovin, N. (2018). Rapid one-step biotinylation of biological and non-biological surfaces. Scientific Reports, 8(1), Article 2845. https://doi.org/10.1038/s41598-018-21186-3
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 31, 2018 |
Publication Date | Feb 12, 2018 |
Deposit Date | Jun 6, 2018 |
Publicly Available Date | Jun 6, 2018 |
Journal | Scientific Reports |
Electronic ISSN | 2045-2322 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 1 |
Article Number | 2845 |
DOI | https://doi.org/10.1038/s41598-018-21186-3 |
Public URL | https://nottingham-repository.worktribe.com/output/911223 |
Publisher URL | https://doi.org/10.1038/s41598-018-21186-3 |
Contract Date | Jun 6, 2018 |
Files
Scientific Reports Biotinilysation.pdf
(1.1 Mb)
PDF
Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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