Plasmonic-based impedance microspectroscopy of optically heterogeneous samples

Abayzeed, Sidahmed A.

Abstract

A robust impedance microscopy technique is presented. This optical tool enables high resolution imaging of electrical properties with promising biophysical applications. The underlying principle is that surface plasmon resonance (SPR) sensors are able to measure perturbations of surface charge density and therefore can be used to compute the impedance of surface-adhered cells. However, the ability to perform reliable quantitative impedance imaging is affected by the optical heterogeneity of the cell-sensor interface. To address this issue, a novel method for quantitative time-resolved resonance angle tracking is developed and applied to correct for the effect of the optical properties. To demonstrate the capability of this technique, impedance microspectroscopy of bovine serum albumin (BSA) patterns was performed enabling measurements of capacitance with submicroscopic resolution. The work presented offers an impedance microspectroscopy method that will create new avenues in studying the electrical properties of single cells and biomolecules as well as bio-electrical currents.

Citation

Abayzeed, S. A. (2020). Plasmonic-based impedance microspectroscopy of optically heterogeneous samples. Biomedical Optics Express, 11(11), 6168-6180. https://doi.org/10.1364/boe.395474

• Journal Article Type: Article
• Acceptance Date: Jun 22, 2020
• Online Publication Date: Oct 8, 2020
• Publication Date: Nov 1, 2020
• Deposit Date: Oct 30, 2020
• Publicly Available Date: Oct 30, 2020
• Journal: Biomedical Optics Express
• Electronic ISSN: 2156-7085
• Publisher: Optical Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 11
• Pages: 6168-6180
• DOI: https://doi.org/10.1364/boe.395474
• Keywords: Biotechnology; Atomic and Molecular Physics, and Optics
• Public URL: https://nottingham-repository.worktribe.com/output/5007472
• Publisher URL: https://www.osapublishing.org/boe/fulltext.cfm?uri=boe-11-11-6168&id=440949
• Additional Information: This article is maintained by: OSA - The Optical Society; Crossref DOI link to publisher maintained version: https://doi.org/10.1364/BOE.395474; Article type: research-article; Similarity check: Screened by Similarity Check; Peer reviewed: Yes; Review process: Single blind; Received: 17 April 2020; Accepted: 22 June 2020; Published: 8 October 2020; Copyright: Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.