Exercising D. melanogaster Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex

Ebanks, Brad; Wang, Ying; Katyal, Gunjan; Sargent, Chloe; Ingram, Thomas L.; Bowman, Antonia; Moisoi, Nicoleta; Chakrabarti, Lisa

Abstract

Ageing is a major risk factor for many of the most prevalent diseases, including neurode-generative diseases, cancer, and heart disease. As the global population continues to age, behavioural interventions that can promote healthy ageing will improve quality of life and relieve the socioeco-nomic burden that comes with an aged society. Exercise is recognised as an effective intervention against many diseases of ageing, but we do not know the stage in an individual’s lifetime at which exercise is most effective at promoting healthy ageing, and whether or not it has a direct effect on lifespan. We exercised w1118 Drosophila melanogaster, investigating the effects of sex and group size at different stages of their lifetime, and recorded their lifespan. Climbing scores at 30 days were measured to record differences in fitness in response to exercise. We also assessed the mitochondrial proteome of w1118 Drosophila that had been exercised for one week, alongside mitochondrial respiration measured using high-resolution respirometry, to determine changes in mitochondrial physiology in response to exercise. We found that age-targeted exercise interventions improved the lifespan of both male and female Drosophila, and grouped males exercised in late life had improved climbing scores when compared with those exercised throughout their entire lifespan. The proteins of the electron transport chain were significantly upregulated in expression after one week of exercise, and complex-II-linked respiration was significantly increased in exercised Drosophila. Taken together, our findings provide a basis to test specific proteins, and complex II of the respiratory chain, as important effectors of exercise-induced healthy ageing.

Citation

Ebanks, B., Wang, Y., Katyal, G., Sargent, C., Ingram, T. L., Bowman, A., …Chakrabarti, L. (2021). Exercising D. melanogaster Modulates the Mitochondrial Proteome and Physiology. The Effect on Lifespan Depends upon Age and Sex. International Journal of Molecular Sciences, 22(21), Article 11606. https://doi.org/10.3390/ijms222111606

• Journal Article Type: Article
• Acceptance Date: Oct 25, 2021
• Online Publication Date: Oct 27, 2021
• Publication Date: Nov 1, 2021
• Deposit Date: Oct 29, 2021
• Publicly Available Date: Oct 29, 2021
• Journal: International Journal of Molecular Sciences
• Print ISSN: 1661-6596
• Electronic ISSN: 1422-0067
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 21
• Article Number: 11606
• DOI: https://doi.org/10.3390/ijms222111606
• Keywords: Inorganic Chemistry; Organic Chemistry; Physical and Theoretical Chemistry; Computer Science Applications; Spectroscopy; Molecular Biology; General Medicine; Catalysis
• Public URL: https://nottingham-repository.worktribe.com/output/6544240
• Publisher URL: https://www.mdpi.com/1422-0067/22/21/11606