Skip to main content

Research Repository

Advanced Search

Analysis of mitochondrial haemoglobin in Parkinson's disease brain

Shephard, Freya; Greville-Heygate, Oliver; Liddell, Susan; Emes, Richard D.; Chakrabarti, Lisa

Analysis of mitochondrial haemoglobin in Parkinson's disease brain Thumbnail


Authors

Freya Shephard

Oliver Greville-Heygate

Susan Liddell

Richard D. Emes

Profile image of LISA CHAKRABARTI

LISA CHAKRABARTI LISA.CHAKRABARTI@NOTTINGHAM.AC.UK
Professor of Mitochondrial Biology



Abstract

Mitochondrial dysfunction is an early feature of neurodegeneration. We have shown there are mitochondrial haemoglobin changes with age and neurodegeneration. We hypothesised that altered physiological processes are associated with recruitment and localisation of haemoglobin to these organelles. To confirm a dynamic localisation of haemoglobin we exposed Drosophila melanogaster to cyclical hypoxia with recovery. With a single cycle of hypoxia and recovery we found a relative accumulation of haemoglobin in the mitochondria compared with the cytosol. An additional cycle of hypoxia and recovery led to a significant increase of mitochondrial haemoglobin (p b 0.05). We quantified ratios of human mitochondrial haemoglobin in 30 Parkinson's and matched control human post-mortem brains. Relative mitochondrial/cytosolic quantities of haemoglobin were obtained for the cortical region, substantia nigra and cerebellum. In age matched postmortem brain mitochondrial haemoglobin ratios change, decreasing with disease duration in female cerebellum samples (n = 7). The change is less discernible in male cerebellum (n = 18). In cerebellar mitochondria, haemoglobin localisation in males with long disease duration shifts from the intermembrane space to the
outer membrane of the organelle. These new data illustrate dynamic localisation of mitochondrial haemoglobin within the cell. Mitochondrial
haemoglobin should be considered in the context of gender differences characterised in Parkinson's disease. It has been postulated that cerebellar circuitry may be activated to play a protective role in individuals with Parkinson's. The changing localisation of intracellular haemoglobin in response to hypoxia presents a novel pathway to delineate the role of the cerebellum in Parkinson's disease.

Citation

Shephard, F., Greville-Heygate, O., Liddell, S., Emes, R. D., & Chakrabarti, L. (2016). Analysis of mitochondrial haemoglobin in Parkinson's disease brain. Mitochondrion, 29, 45-52. https://doi.org/10.1016/j.mito.2016.05.001

Journal Article Type Article
Acceptance Date May 3, 2016
Online Publication Date May 12, 2016
Publication Date Jul 1, 2016
Deposit Date Jun 6, 2016
Publicly Available Date Jun 6, 2016
Journal Mitochondrion
Print ISSN 1567-7249
Electronic ISSN 1872-8278
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 29
Pages 45-52
DOI https://doi.org/10.1016/j.mito.2016.05.001
Keywords Parkinson's disease; Mitochondria; Haemoglobin; Gender; Cerebellum; Hypoxia
Public URL https://nottingham-repository.worktribe.com/output/792443
Publisher URL http://www.sciencedirect.com/science/article/pii/S1567724916300423
Additional Information This article is maintained by: Elsevier; Article Title: Analysis of Mitochondrial haemoglobin in Parkinson's disease brain; Journal Title: Mitochondrion; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.mito.2016.05.001; Content Type: article; Copyright: © 2016 The Authors. Published by Elsevier B.V.
Contract Date Jun 6, 2016

Files





You might also like



Downloadable Citations