Dr HELEN KNIGHT's Outputs (9)

Global co-regulatory cross talk between m6A and m5C RNA methylation systems coordinate cellular responses and brain disease pathways (2024)
Journal Article
Chukwuma Orji, O., Stones, J., Bagia, S., Markus, R., Demirbugen Öz, M., & Knight, H. (in press). Global co-regulatory cross talk between m6A and m5C RNA methylation systems coordinate cellular responses and brain disease pathways. Molecular Neurobiology,

N6 adenosine and C5 cytosine modification of mRNAs, tRNAs and rRNAs are regulated by the behaviour of distinct sets of writer, reader and eraser effector proteins which are conventionally considered to function independently. Here, we provide evidenc... Read More about Global co-regulatory cross talk between m6A and m5C RNA methylation systems coordinate cellular responses and brain disease pathways.

The expanding role of cap-adjacent modifications in animals (2024)
Journal Article
Bellows, E., Fray, R. G., Knight, H. M., & Archer, N. (2024). The expanding role of cap-adjacent modifications in animals. Frontiers in RNA Research, 2, Article 1485307. https://doi.org/10.3389/frnar.2024.1485307

Eukaryotic mRNA cap structures directly influence mRNA stability, translation, and immune recognition. While the significance of the mRNA cap itself has been well-established, recent research has revealed the intricate modifications to the nucleotide... Read More about The expanding role of cap-adjacent modifications in animals.

Dysregulation of RNA modification systems in clinical populations with neurocognitive disorders (2023)
Journal Article
KNIGHT, H., Öz, M. D., & PerezGrovas-Saltijeral, A. (2024). Dysregulation of RNA modification systems in clinical populations with neurocognitive disorders. Neural Regeneration Research, 19(6), 1256-1261. https://doi.org/10.4103/1673-5374.385858

The study of modified RNA known as epitranscriptomics has become increasingly relevant in our understanding of disease-modifying mechanisms. Methylation of N6 adenosine (m 6 A) and C5 cytosine (m 5 C) bases occur on mRNAs, tRNA, mt-tRNA, and rRNA spe... Read More about Dysregulation of RNA modification systems in clinical populations with neurocognitive disorders.

Extracellular vesicle microRNA mediated transcriptional regulation may contribute to Dementia with Lewy Bodies molecular pathology (2023)
Journal Article
Işık, F. B., Knight, H. M., & Rajkumar, A. P. (2024). Extracellular vesicle microRNA mediated transcriptional regulation may contribute to Dementia with Lewy Bodies molecular pathology. Acta Neuropsychiatrica, 36(1), 29-38. https://doi.org/10.1017/neu.2023.27

Objective:

Dementia with Lewy bodies (DLB) is the second most common dementia. Advancing our limited understanding of its molecular pathogenesis is essential for identifying novel biomarkers and therapeutic targets for DLB. DLB is an α-synucleinop... Read More about Extracellular vesicle microRNA mediated transcriptional regulation may contribute to Dementia with Lewy Bodies molecular pathology.

m6A mRNA methylation in human brain is disrupted in Lewy body disorders (2023)
Journal Article
Martinez De La Cruz, B., Gell, C., Markus, R., Macdonald, I., Fray, R., & Knight, H. M. (2023). m6A mRNA methylation in human brain is disrupted in Lewy body disorders. Neuropathology and Applied Neurobiology, 49(1), Article e12885. https://doi.org/10.1111/nan.12885

N6-methyladenosine modification of RNA (m6A) regulates translational control which may influence neuronal dysfunction underlying neurodegenerative diseases. Using microscopy and a machine learning approach, we performed cellular profiling of m6A-RNA... Read More about m6A mRNA methylation in human brain is disrupted in Lewy body disorders.

The importance of m6A topology in chicken embryo mRNA: a precise mapping of m6A at the conserved chicken β-actin zipcode (2023)
Journal Article
Baron, F., Zhang, M., Archer, N., Bellows, E., Knight, H. M., Welham, S., …Bodi, Z. (2023). The importance of m6A topology in chicken embryo mRNA: a precise mapping of m6A at the conserved chicken β-actin zipcode. RNA, 29(6), 777-789. https://doi.org/10.1261/rna.079615.123

N6-methyladenosine (m6A) in mRNA regulates almost every stage in the mRNA life cycle, and the development of methodologies for the high-throughput detection of methylated sites in mRNA using m6A-specific methylated RNA immunoprecipitation with next-g... Read More about The importance of m6A topology in chicken embryo mRNA: a precise mapping of m6A at the conserved chicken β-actin zipcode.

Differential expression of m5C RNA methyltransferase genes NSUN6 and NSUN7 in Alzheimer’s disease and traumatic brain injury (2023)
Journal Article
Perez Grovas-Saltijeral, A., Rajkumar, A. P., & Knight, H. M. (2023). Differential expression of m5C RNA methyltransferase genes NSUN6 and NSUN7 in Alzheimer’s disease and traumatic brain injury. Molecular Neurobiology, https://doi.org/10.1007/s12035-022-03195-6

Epigenetic processes have become increasingly relevant in understanding disease-modifying mechanisms. 5-Methylcytosine methylations of DNA (5mC) and RNA (m5C) have functional transcriptional and RNA translational consequences and are tightly regulate... Read More about Differential expression of m5C RNA methyltransferase genes NSUN6 and NSUN7 in Alzheimer’s disease and traumatic brain injury.

Damaging coding variants within kainate receptor channel genes are enriched in individuals with schizophrenia, autism and intellectual disabilities (2019)
Journal Article
Koromina, M., Flitton, M., Blockley, A., Mellor, I. R., & Knight, H. (2019). Damaging coding variants within kainate receptor channel genes are enriched in individuals with schizophrenia, autism and intellectual disabilities. Scientific Reports, 9, Article 19215. https://doi.org/10.1038/s41598-019-55635-4

Schizophrenia (Scz), autism spectrum disorder (ASD) and intellectual disability are common complex neurodevelopmental disorders. Kainate receptors (KARs) are ionotropic glutamate ion channels involved in synaptic plasticity which are modulated by aux... Read More about Damaging coding variants within kainate receptor channel genes are enriched in individuals with schizophrenia, autism and intellectual disabilities.

A kainate receptor GluK4 deletion, protective against bipolar disorder, is associated with enhanced cognitive performance across diagnoses in the TwinsUK cohort (2018)
Journal Article
Koromina, M., Flitton, M., Mellor, I. R., & Knight, H. M. (2019). A kainate receptor GluK4 deletion, protective against bipolar disorder, is associated with enhanced cognitive performance across diagnoses in the TwinsUK cohort. World Journal of Biological Psychiatry, 20(5), 393-401. https://doi.org/10.1080/15622975.2017.1417637

Objectives: Cognitive deficits are a common feature of neuropsychiatric disorders. We investigated the relationship between cognitive performance and a deletion allele within GluK4 protective against risk for bipolar disorder, in 1642 individuals fro... Read More about A kainate receptor GluK4 deletion, protective against bipolar disorder, is associated with enhanced cognitive performance across diagnoses in the TwinsUK cohort.