Professor HANY ELSHEIKHA's Outputs (159)

In Silico–Based Investigation of the Immunogenicity and Biochemical Attributes of Toxoplasma gondii Apical Membrane Antigen 1 (TgAMA1) (2025)
Journal Article
Foroutan, M., Ghaffari, A. D., Ghaffarifar, F., Karimipour-Saryazdi, A., Birgani, A. A., Majidiani, H., Cortes, H., & Elsheikha, H. M. (2025). In Silico–Based Investigation of the Immunogenicity and Biochemical Attributes of Toxoplasma gondii Apical Membrane Antigen 1 (TgAMA1). Journal of Parasitology Research, https://doi.org/10.1155/japr/3514414

Background: Apical membrane antigen 1 (AMA1) is a highly conserved microneme protein in apicomplexan parasites. In this study, immunoinformatics tools and in silico protein structure prediction were used to characterize the structure, physicochemical... Read More about In Silico–Based Investigation of the Immunogenicity and Biochemical Attributes of Toxoplasma gondii Apical Membrane Antigen 1 (TgAMA1).

Investigation of the genetic diversity of gut mycobiota of the wild and laboratory mice (2025)
Journal Article
Zhao, J.-X., Elsheikha, H. M., Shang, K.-M., Su, J.-W., Wei, Y.-J., Qin, Y., Zhao, Z.-Y., Ma, H., & Zhang, X.-X. (2025). Investigation of the genetic diversity of gut mycobiota of the wild and laboratory mice. Microbiology Spectrum, Article e02840-24. https://doi.org/10.1128/spectrum.02840-24

Mice are colonized by diverse gut fungi, known as the mycobiota, which have received much less attention than bacterial microbiota. Here, we studied the diversities and structures of cecal fungal communities in wild (Lasiopodomys brandtii, Apodemus a... Read More about Investigation of the genetic diversity of gut mycobiota of the wild and laboratory mice.

Neuroinflammation and schizophrenia: The role of Toxoplasma gondii infection and astrocytic dysfunction (2025)
Journal Article
Everett, A., & Elsheikha, H. M. (2025). Neuroinflammation and schizophrenia: The role of Toxoplasma gondii infection and astrocytic dysfunction. Journal of Neuroimmunology, 403, Article 578588. https://doi.org/10.1016/j.jneuroim.2025.578588

Obligate intracellular pathogens such as the protozoan Toxoplasma gondii exploit host cell mechanisms to facilitate their survival and replication. While T. gondii can infect any nucleated mammalian cell, it exhibits a particular affinity for central... Read More about Neuroinflammation and schizophrenia: The role of Toxoplasma gondii infection and astrocytic dysfunction.

Morphological and Chemical Changes in the Trophozoites and Cysts of Acanthamoeba Castellanii Induced by Camellia Sinensis Extracts (2025)
Journal Article
Fakae, L. B., Zhong, J., Chan, K. L. A., Mekapothula, S., Cave, G. W., Zhu, X.-Q., Stevenson, C. W., & Elsheikha, H. M. (2025). Morphological and Chemical Changes in the Trophozoites and Cysts of Acanthamoeba Castellanii Induced by Camellia Sinensis Extracts. Acta Parasitologica, 70(2), Article 63. https://doi.org/10.1007/s11686-024-00941-9

Purpose Acanthamoeba castellanii is an important opportunistic human protozoal pathogen that can cause both skin, ocular and brain infections. Recent studies have established that brews and solvent extract (SE) of green tea (Camellia sinensis) can in... Read More about Morphological and Chemical Changes in the Trophozoites and Cysts of Acanthamoeba Castellanii Induced by Camellia Sinensis Extracts.

Prevalence, risk factors, and regional insights of bovine fasciolosis in China: A systematic review and meta-analysis (2025)
Journal Article
Lan, Z., Zhang, Y.-X., Zhang, A.-H., Wang, Y.-Y., Qiu, H.-Y., Gao, J.-F., Cheng, G.-F., Elsheikha, H., & Wang, C.-R. (2025). Prevalence, risk factors, and regional insights of bovine fasciolosis in China: A systematic review and meta-analysis. Acta Tropica, 263, Article 107570. https://doi.org/10.1016/j.actatropica.2025.107570

Fasciolosis, a significant zoonotic disease with considerable economic and public health implications, presents a serious challenge to both animal husbandry and human health. Despite numerous reports on bovine fasciolosis, a comprehensive understandi... Read More about Prevalence, risk factors, and regional insights of bovine fasciolosis in China: A systematic review and meta-analysis.

In Silico Analysis and Characterization of the Immunogenicity of Toxoplasma gondii Rhoptry Protein 18 (2025)
Journal Article
Foroutan, M., Karimipour-Saryazdi, A., Dalir Ghaffari, A., Majidiani, H., Arzani Birgani, A., Karimzadeh-Soureshjani, E., Soltani, S., & Elsheikha, H. M. (2025). In Silico Analysis and Characterization of the Immunogenicity of Toxoplasma gondii Rhoptry Protein 18. Bioinformatics and Biology Insights, 19, https://doi.org/10.1177/11779322251315924

Rhoptry protein 18 (ROP18) is a key virulence factor secreted into host cells during the invasion of Toxoplasma gondii (T. gondii) and plays an important role in the pathogenesis of infection. Due to its potential as a vaccine candidate, this study a... Read More about In Silico Analysis and Characterization of the Immunogenicity of Toxoplasma gondii Rhoptry Protein 18.

A novel cross-priming amplification technique combined with lateral flow strips for rapid and visual detection of zoonotic Toxoplasma gondii (2025)
Journal Article
Liang, Y., He, Y.-H., Yang, S.-F., Xie, S.-C., Lv, Y.-H., Cong, W., Elsheikha, H. M., & Zhu, X.-Q. (2025). A novel cross-priming amplification technique combined with lateral flow strips for rapid and visual detection of zoonotic Toxoplasma gondii. Veterinary Parasitology, 334, Article 110402. https://doi.org/10.1016/j.vetpar.2025.110402

Toxoplasma gondii, an obligate intracellular protozoan, infects almost all warm-blooded animals and humans, with felines serving as its sole definitive hosts. Cats release T. gondii oocysts into the environment through feces, contributing to environm... Read More about A novel cross-priming amplification technique combined with lateral flow strips for rapid and visual detection of zoonotic Toxoplasma gondii.

Characteristics of fecal mycobiota and bacteriota in laying hens during different laying periods (2025)
Journal Article
Wei, Y.-J., Shang, K.-M., Elsheikha, H. M., Yan, J.-C., Zhao, J.-X., Ma, H., Geng, H.-L., Meng, J.-X., Li, W.-D., Liu, R., Zhang, X.-X., & Ni, H.-B. (2025). Characteristics of fecal mycobiota and bacteriota in laying hens during different laying periods. Microbial Pathogenesis, 200, Article 107304. https://doi.org/10.1016/j.micpath.2025.107304

Mycobiota represents an important component of the gut microbiome in poultry and plays important roles in host nutrition and metabolism. However, the understanding of gut mycobiota in laying hens during the production cycle is limited. The present st... Read More about Characteristics of fecal mycobiota and bacteriota in laying hens during different laying periods.

Glabridin exhibits potent inhibitory effects against Toxoplasma gondii in vitro and in vivo (2024)
Journal Article
Wang, L., Zhai, B., Wang, C., Elsheikha, H. M., Guo, H., Zheng, X.-N., Zhou, C., & Zhu, X.-Q. (2024). Glabridin exhibits potent inhibitory effects against Toxoplasma gondii in vitro and in vivo. Parasites and Vectors, 17(1), Article 522. https://doi.org/10.1186/s13071-024-06610-0

Background: Toxoplasma gondii is an obligate protozoan parasite capable of infecting a wide range of warm-blooded animals and humans. Current treatment options, primarily pyrimethamine and sulfadiazine, have limitations, such as high recurrence rates... Read More about Glabridin exhibits potent inhibitory effects against Toxoplasma gondii in vitro and in vivo.

A novel single-tube LAMP-CRISPR/Cas12b method for rapid and visual detection of zoonotic Toxoplasma gondii in the environment (2024)
Journal Article
Liang, Y., Xie, S.-C., Lv, Y.-H., He, Y.-H., Zheng, X.-N., Cong, W., Elsheikha, H. M., & Zhu, X.-Q. (2024). A novel single-tube LAMP-CRISPR/Cas12b method for rapid and visual detection of zoonotic Toxoplasma gondii in the environment. Infectious Diseases of Poverty, 13(1), Article 94. https://doi.org/10.1186/s40249-024-01266-5

Background: Toxoplasma gondii oocysts, excreted in cat feces, pose a significant health risk to humans through contaminated soil and water. Rapid and accurate detection of T. gondii in environmental samples is essential for public health protection.... Read More about A novel single-tube LAMP-CRISPR/Cas12b method for rapid and visual detection of zoonotic Toxoplasma gondii in the environment.

Comprehensive analysis of the global impact and distribution of tick paralysis, a deadly neurological yet fully reversible condition (2024)
Journal Article
Deng, Y.-P., Fu, Y.-T., Elsheikha, H. M., Cao, M.-L., Zhu, X.-Q., Wang, J.-L., Zhang, X., Xie, S.-C., Yao, C., & Liu, G.-H. (2024). Comprehensive analysis of the global impact and distribution of tick paralysis, a deadly neurological yet fully reversible condition. Clinical Microbiology Reviews, 37(4), Article e00074-24. https://doi.org/10.1128/cmr.00074-24

Tick paralysis is a potentially fatal condition caused by neurotoxins secreted by the salivary glands of certain ticks. Documented cases have been reported worldwide, predominantly in the United States, Canada, and Australia, with additional reports... Read More about Comprehensive analysis of the global impact and distribution of tick paralysis, a deadly neurological yet fully reversible condition.

Metagenomic profiling of cecal microbiota and antibiotic resistome in rodents (2024)
Journal Article
Shang, K.-M., Elsheikha, H. M., Ma, H., Wei, Y.-J., Zhao, J.-X., Qin, Y., Li, J.-M., Zhao, Z.-Y., & Zhang, X.-X. (2024). Metagenomic profiling of cecal microbiota and antibiotic resistome in rodents. Ecotoxicology and Environmental Safety, 286, Article 117186. https://doi.org/10.1016/j.ecoenv.2024.117186

The rodent gut microbiota is a known reservoir of antimicrobial resistance, yet the distribution of antibiotic resistance genes (ARGs) within rodent cecal microbial communities and the specific bacterial species harboring these ARGs remain largely un... Read More about Metagenomic profiling of cecal microbiota and antibiotic resistome in rodents.

Urban estuary serves as a critical nexus for the land-sea transfer of the terrestrial pathogen Toxoplasma gondii (2024)
Journal Article
Zhu, X.-K., Elsheikha, H. M., Yang, T., Li, M.-Y., & Cong, W. (2024). Urban estuary serves as a critical nexus for the land-sea transfer of the terrestrial pathogen Toxoplasma gondii. Science of the Total Environment, 955, Article 176983. https://doi.org/10.1016/j.scitotenv.2024.176983

Terrestrial runoff is a key pathway for the transmission of the terrestrial pathogen Toxoplasma gondii from land to sea, posing a significant threat to marine ecosystems. Understanding the mechanisms by which T. gondii is transported from terrestrial... Read More about Urban estuary serves as a critical nexus for the land-sea transfer of the terrestrial pathogen Toxoplasma gondii.

A Colour Atlas of Companion Animal Parasites: Life Cycles and Morphological Identification (2024)
Book
McGarry, J. W., Elsheikha, H. M., & Taylor, S. (2024). A Colour Atlas of Companion Animal Parasites: Life Cycles and Morphological Identification. CAB International

Functional Characterization of Six Eukaryotic Translation Initiation Factors of Toxoplasma gondii Using the CRISPR-Cas9 System (2024)
Journal Article
Kou, Y.-J., Gao, J., Li, R., Ma, Z.-Y., Elsheikha, H. M., Wu, X.-J., Zheng, X.-N., Wang, M., & Zhu, X.-Q. (2024). Functional Characterization of Six Eukaryotic Translation Initiation Factors of Toxoplasma gondii Using the CRISPR-Cas9 System. International Journal of Molecular Sciences, 25(14), Article 7834. https://doi.org/10.3390/ijms25147834

Eukaryotic translation initiation factors (eIFs) are crucial for initiating protein translation and ensuring the correct assembly of mRNA-ribosomal subunit complexes. In this study, we investigated the effects of deleting six eIFs in the apicomplexan... Read More about Functional Characterization of Six Eukaryotic Translation Initiation Factors of Toxoplasma gondii Using the CRISPR-Cas9 System.

Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii (2024)
Journal Article
Zhang, X.-X., Sun, Y.-Z., Wang, W., Gao, Y., Wei, X.-Y., Sun, H.-C., Wang, C.-R., Ni, H.-B., Yang, X., Elsheikha, H. M., & Guo, H.-P. (2024). Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii. PLoS Neglected Tropical Diseases, 18(6), Article e0012281. https://doi.org/10.1371/journal.pntd.0012281

Background

Pathogens can impact host RNA modification machinery to establish a favorable cellular environment for their replication. In the present study, we investigated the effect of Toxoplasma gondii infection on host RNA modification profiles... Read More about Altered landscape of total RNA, tRNA and sncRNA modifications in the liver and spleen of mice infected by Toxoplasma gondii.

Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells (2024)
Journal Article
Alhazmi, A., Sidney, L. E., Hopkinson, A., & Elsheikha, H. M. (2024). Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells. Acta Tropica, 257, Article 107288. https://doi.org/10.1016/j.actatropica.2024.107288

Soluble factors in the secretome of Acanthamoeba castellanii play crucial roles in the pathogenesis of Acanthamoeba keratitis (AK). Investigating the pathological effects of A. castellanii-derived conditioned medium (ACCM) on ocular cells can provide... Read More about Comparative cytotoxicity of Acanthamoeba castellanii-derived conditioned medium on human corneal epithelial and stromal cells.

Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness (2024)
Journal Article
Zhang, Z.-W., Wang, M., Sun, L.-X., Elsheikha, H. M., Lei, C.-L., Wang, J.-L., Fu, B.-Q., Luo, J.-X., Zhu, X.-Q., & Li, T.-T. (2024). Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness. Parasites and Vectors, 17(1), Article 178. https://doi.org/10.1186/s13071-024-06259-9

Background To successfully replicate within the host cell, Toxoplasma gondii employs several mechanisms to overcome the host cell defenses and mitigate the harmful effects of the free radicals resulting from its own metabolic processes using effector... Read More about Trx4, a novel thioredoxin protein, is important for Toxoplasma gondii fitness.

Global prevalence and risk factors associated with Toxoplasma gondii infection in wild birds: A systematic review and meta-analysis (2024)
Journal Article
Chen, C., Qin, S.-Y., Yang, X., Li, X.-M., Cai, Y., Lei, C.-C., Zhao, Q., Elsheikha, H. M., & Cao, H. (2024). Global prevalence and risk factors associated with Toxoplasma gondii infection in wild birds: A systematic review and meta-analysis. Preventive Veterinary Medicine, 226, Article 106187. https://doi.org/10.1016/j.prevetmed.2024.106187

A systematic review and meta-analysis were performed to identify the global prevalence and factors associated with Toxoplasma gondii infection in wild birds. Six bibliographic databases (Chinese National Knowledge Infrastructure, VIP Chinese Journal... Read More about Global prevalence and risk factors associated with Toxoplasma gondii infection in wild birds: A systematic review and meta-analysis.

Expression profiles of host miRNAs and circRNAs and ceRNA network during Toxoplasma gondii lytic cycle (2024)
Journal Article
Wang, S.-S., Wang, X., He, J.-J., Zheng, W.-B., Zhu, X.-Q., Elsheikha, H. M., & Zhou, C.-X. (2024). Expression profiles of host miRNAs and circRNAs and ceRNA network during Toxoplasma gondii lytic cycle. Parasitology Research, 123(2), Article 145. https://doi.org/10.1007/s00436-024-08152-x

Toxoplasma gondii is an opportunistic protozoan parasite that is highly prevalent in the human population and can lead to adverse health consequences in immunocompromised patients and pregnant women. Noncoding RNAs, such as microRNAs (miRNAs) and cir... Read More about Expression profiles of host miRNAs and circRNAs and ceRNA network during Toxoplasma gondii lytic cycle.