Professor CHRIS DENNING's Outputs (6)

Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion (2020)
Journal Article
Nasir, A., Thorpe, J., Burroughs, L., Meurs, J., Pijuan‐Galito, S., Irvine, D. J., Alexander, M. R., & Denning, C. (2020). Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion. Advanced Healthcare Materials, 10(6), Article 2001448. https://doi.org/10.1002/adhm.202001448

Human pluripotent stem cells (hPSCs) can be expanded and differentiated in vitro into almost any adult tissue cell type, and thus have great potential as a source for cell therapies with biomedical application. In this study, a fully-defined polymer... Read More about Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion.

Experiences of the COVID-19 pandemic: cross-sectional analysis of risk perceptions and mental health in a student cohort (2020)
Preprint / Working Paper
Jia, R., Knight, H., Blake, H., Corner, J., Denning, C., Ball, J., Bolton, K., Morling, J. R., Coupland, C., Figueredo, G., Morris, D. E., Tighe, P., Villalon, A., Aylling, K., & Vedhara, K. Experiences of the COVID-19 pandemic: cross-sectional analysis of risk perceptions and mental health in a student cohort

Objective: This study examined the COVID-19 risk perceptions and mental health of university students on returning to campus in the midst of the COVID-19 pandemic.

Methods: An online survey was completed during the first four weeks of the academic... Read More about Experiences of the COVID-19 pandemic: cross-sectional analysis of risk perceptions and mental health in a student cohort.

CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes (2020)
Journal Article
Kondrashov, A., Mohd Yusof, N. A., Hasan, A., Goulding, J., Kodagoda, T., Hoang, D. M., Vo, N. T., Melarangi, T., Dolatshad, N., Gorelik, J., Hill, S. J., Harding, S. E., & Denning, C. (2021). CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes. Molecular Therapy - Methods and Clinical Development, 20, 39-53. https://doi.org/10.1016/j.omtm.2020.10.019

© 2020 During normal- and patho-physiological situations, the behavior of the beta2-adrenoreceptor (β2AR) is influenced by polymorphic variants. The functional impact of such polymorphisms has been suggested from data derived from genetic association... Read More about CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes.

Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent (2020)
Journal Article
Bodbin, S. E., Denning, C., & Mosqueira, D. (2020). Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent. Methods and Protocols, 3(3), Article 57. https://doi.org/10.3390/mps3030057

Twenty years since their first derivation, human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) have shown promise in disease modelling research, while their potential for cardiac repair is being investigated. However, low transfection effic... Read More about Transfection of hPSC-Cardiomyocytes Using Viafect™ Transfection Reagent.

Mitochondrial DNA: Hotspot for Potential Gene Modifiers Regulating Hypertrophic Cardiomyopathy (2020)
Journal Article
Kargaran, P. K., Evans, J. M., Bodbin, S. E., Smith, J. G. W., Nelson, T. J., Denning, C., & Mosqueira, D. (2020). Mitochondrial DNA: Hotspot for Potential Gene Modifiers Regulating Hypertrophic Cardiomyopathy. Journal of Clinical Medicine, 9(8), Article 2349. https://doi.org/10.3390/jcm9082349

Hypertrophic cardiomyopathy (HCM) is a prevalent and untreatable cardiovascular disease with a highly complex clinical and genetic causation. HCM patients bearing similar sarcomeric mutations display variable clinical outcomes, implying the involveme... Read More about Mitochondrial DNA: Hotspot for Potential Gene Modifiers Regulating Hypertrophic Cardiomyopathy.

Blinded, multi-centre evaluation of drug-induced changes in contractility using human induced pluripotent stem cell-derived cardiomyocytes (2020)
Journal Article
Saleem, U., van Meer, B. J., Katili, P. A., Yusof, N. A. N. M., Mannhardt, I., Garcia, A. K., Tertoolen, L., de Korte, T., Vlaming, M. L., McGlynn, K., Nebel, J., Bahinski, A., Harris, K., Rossman, E., Xu, X., Burton, F. L., Smith, G. L., Clements, P., Mummery, C. L., Eschenhagen, T., …Denning, C. (2020). Blinded, multi-centre evaluation of drug-induced changes in contractility using human induced pluripotent stem cell-derived cardiomyocytes. Toxicological Sciences, 176(1), 103–123. https://doi.org/10.1093/toxsci/kfaa058

Animal models are 78% accurate in determining whether drugs will alter contractility of the human heart. To evaluate the suitability of human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) for predictive safety pharmacology, we quan... Read More about Blinded, multi-centre evaluation of drug-induced changes in contractility using human induced pluripotent stem cell-derived cardiomyocytes.