All Outputs (259)

Immune-instructive materials and surfaces for medical applications (2021)
Book Chapter
Fisher, L. E., & Ghaemmaghami, A. M. (2021). Immune-instructive materials and surfaces for medical applications. In S. F. Badylak, & J. H. Elisseeff (Eds.), Immunomodulatory Biomaterials: Regulating the Immune Response with Biomaterials to Affect Clinical Outcome (67-87). Elsevier. https://doi.org/10.1016/B978-0-12-821440-4.00008-6

Biomaterials are an integral part of modern medicine with diverse applications including drug delivery platforms, scaffolds for tissue engineering, biosensors and medical devices/implants. The aging population and steady increase in the prevalence of... Read More about Immune-instructive materials and surfaces for medical applications.

Practical Recommendations for High-Intensity Interval Training for Adults with Cardiovascular Disease (2021)
Journal Article
Way, K. L., Terada, T., O’Neill, C. D., Vidal-Almela, S., Keech, A., & Reed, J. L. (2021). Practical Recommendations for High-Intensity Interval Training for Adults with Cardiovascular Disease. ACSM's Health and Fitness Journal, 25(5), 35-43. https://doi.org/10.1249/FIT.0000000000000705

Apply It!

• High-intensity interval training (HIIT) can be implemented safely in adults with cardiovascular disease (CVD) after a graded exercise test assessing exercise responses at a high intensity (e.g., to 85% heart rate peak [HRpeak] or a ra... Read More about Practical Recommendations for High-Intensity Interval Training for Adults with Cardiovascular Disease.

Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices (2021)
Journal Article
He, Y., Luckett, J., Begines, B., Dubern, J. F., Hook, A. L., Prina, E., Rose, F. R., Tuck, C. J., Hague, R. J., Irvine, D. J., Williams, P., Alexander, M. R., & Wildman, R. D. (2022). Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices. Biomaterials, 281, Article 121350. https://doi.org/10.1016/j.biomaterials.2021.121350

Chronic infection as a result of bacterial biofilm formation on implanted medical devices is a major global healthcare problem requiring new biocompatible, biofilm-resistant materials. Here we demonstrate how bespoke devices can be manufactured throu... Read More about Ink-jet 3D printing as a strategy for developing bespoke non-eluting biofilm resistant medical devices.

The rs429358 Locus in Apolipoprotein E Is Associated With Hepatocellular Carcinoma in Patients With Cirrhosis (2021)
Journal Article
Innes, H., Nischalke, H. D., Guha, I. N., Weiss, K. H., Irving, W., Gotthardt, D., Barnes, E., Fischer, J., Ansari, A., Rosendahl, J., Shang-Kuan, L., Marot, A., Pedergnana, V., Casper, M., Benselin, J., Lammert, F., McLauchlan, J., Lutz, P. L., Hamill, V., Mueller, S., …Buch, S. (2022). The rs429358 Locus in Apolipoprotein E Is Associated With Hepatocellular Carcinoma in Patients With Cirrhosis. Hepatology Communications, 6(5), 1213-1226. https://doi.org/10.1002/hep4.1886

The host genetic background for hepatocellular carcinoma (HCC) is incompletely understood. We aimed to determine if four germline genetic polymorphisms, rs429358 in apolipoprotein E (APOE), rs2642438 in mitochondrial amidoxime reducing component 1 (M... Read More about The rs429358 Locus in Apolipoprotein E Is Associated With Hepatocellular Carcinoma in Patients With Cirrhosis.

Immune-instructive materials as new tools for immunotherapy (2021)
Journal Article
Fisher, L. E., Kämmerling, L., Alexander, M. R., & Ghaemmaghami, A. M. (2022). Immune-instructive materials as new tools for immunotherapy. Current Opinion in Biotechnology, 74, 194-203. https://doi.org/10.1016/j.copbio.2021.11.005

Immune instructive materials, are materials with the ability to modulate or mimic the function of immune cells, provide exciting opportunities for developing new therapies in many areas including medical devices, chronic inflammation, cancer, and aut... Read More about Immune-instructive materials as new tools for immunotherapy.

Serum troponin, D‐dimer, and CRP level in severe coronavirus (COVID‐19) patients (2021)
Journal Article
Ali, A. M., Rostam, H. M., Fatah, M. H., Noori, C. M., Ali, K. M., & Tawfeeq, H. M. (2022). Serum troponin, D‐dimer, and CRP level in severe coronavirus (COVID‐19) patients. Immunity, Inflammation and Disease, 10(3), Article e582. https://doi.org/10.1002/iid3.582

Background

Abnormal inflammation coagulation biomarker levels of troponin, C-reactive protein (CRP), and D-dimer levels in serum have been demonstrated to be associated and involved in the disease progression of coronavirus disease 2019 (COVID-19)... Read More about Serum troponin, D‐dimer, and CRP level in severe coronavirus (COVID‐19) patients.

Mutation and selection explain why many eukaryotic centromeric DNA sequences are often A+T rich (2021)
Journal Article
Barbosa, A. C., Xu, Z., Karari, K., Williams, W., Hauf, S., & Brown, W. R. A. (2022). Mutation and selection explain why many eukaryotic centromeric DNA sequences are often A+T rich. Nucleic Acids Research, 50(1), 579-596. https://doi.org/10.1093/nar/gkab1219

We have used chromosome engineering to replace native centromeric DNA with different test sequences at native centromeres in two different strains of the fission yeast Schizosaccharomyces pombe and have discovered that A + T rich DNA, whether synthet... Read More about Mutation and selection explain why many eukaryotic centromeric DNA sequences are often A+T rich.

An ALS-associated variant of the autophagy receptor SQSTM1/p62 reprograms binding selectivity toward the autophagy-related hATG8 proteins (2021)
Journal Article
Brennan, A., Layfield, R., Long, J., Williams, H. E., Oldham, N. J., Scott, D., & Searle, M. S. (2022). An ALS-associated variant of the autophagy receptor SQSTM1/p62 reprograms binding selectivity toward the autophagy-related hATG8 proteins. Journal of Biological Chemistry, 298(2), Article 101514. https://doi.org/10.1016/j.jbc.2021.101514

Recognition of human autophagy-related 8 (hATG8) proteins by autophagy receptors represents a critical step within this cellular quality control system. Autophagy impairment is known to be a pathogenic mechanism in the motor neuron disorder amyotroph... Read More about An ALS-associated variant of the autophagy receptor SQSTM1/p62 reprograms binding selectivity toward the autophagy-related hATG8 proteins.

Saliva for COVID-19 testing: Simple but useless or an undervalued resource? (2021)
Journal Article
Pijuan-Galito, S., Tarantini, F. S., Tomlin, H., Jenkins, H., Thompson, J. L., Scales, D., Stroud, A., Tellechea Lopez, A., Hassall, J., McTernan, P., Coultas, A., Arendt-Tranholm, A., Reffin, C., Hill, I., Lee, I.-N., Wu, S., Porte, J., Chappell, J., Lis-Slimak, K., Kaneko, K., …Denning, C. (2021). Saliva for COVID-19 testing: Simple but useless or an undervalued resource?. Frontiers in Virology, 1, Article 778790. https://doi.org/10.3389/fviro.2021.778790

During the COVID-19 pandemic, countries with robust population-based asymptomatic testing were generally successful in controlling virus spread, hence reducing hospitalizations and deaths. This effectiveness inspired widespread asymptomatic surveilla... Read More about Saliva for COVID-19 testing: Simple but useless or an undervalued resource?.

Differential fates of introns in gene expression due to global alternative splicing (2021)
Journal Article
Kumari, A., Sedehizadeh, S., Brook, J. D., Kozlowski, P., & Wojciechowska, M. (2022). Differential fates of introns in gene expression due to global alternative splicing. Human Genetics, 141, 31-47. https://doi.org/10.1007/s00439-021-02409-6

The discovery of introns over four decades ago revealed a new vision of genes and their interrupted arrangement. Throughout the years, it has appeared that introns play essential roles in the regulation of gene expression. Unique processing of excise... Read More about Differential fates of introns in gene expression due to global alternative splicing.

Neuroprotection Against NMDA-Induced Retinal Damage by Philanthotoxin-343 Involves Reduced Nitrosative Stress (2021)
Journal Article
Mohamad, M. H. N., Abu, I. F., Fazel, M. F., Agarwal, R., Iezhitsa, I., Juliana, N., Mellor, I. R., & Franzyk, H. (2021). Neuroprotection Against NMDA-Induced Retinal Damage by Philanthotoxin-343 Involves Reduced Nitrosative Stress. Frontiers in Pharmacology, 12, Article 798794. https://doi.org/10.3389/fphar.2021.798794

N-methyl-D-aspartate receptor (NMDAR) overstimulation is known to mediate neurodegeneration, and hence represents a relevant therapeutic target for neurodegenerative disorders including glaucoma. This study examined the neuroprotective effects of phi... Read More about Neuroprotection Against NMDA-Induced Retinal Damage by Philanthotoxin-343 Involves Reduced Nitrosative Stress.

Emergent SARS-CoV-2 variants: comparative replication dynamics and high sensitivity to thapsigargin (2021)
Journal Article
Al-Beltagi, S., Goulding, L. V., Chang, D. K., Mellits, K. H., Hayes, C. J., Gershkovich, P., Coleman, C. M., & Chang, K.-C. (2021). Emergent SARS-CoV-2 variants: comparative replication dynamics and high sensitivity to thapsigargin. Virulence, 12(1), 2946-2956. https://doi.org/10.1080/21505594.2021.2006960

The struggle to control the COVID-19 pandemic is made challenging by the emergence of virulent SARS-CoV-2 variants. To gain insight into their replication dynamics, emergent Alpha (A), Beta (B) and Delta (D) SARS-CoV-2 variants were assessed for thei... Read More about Emergent SARS-CoV-2 variants: comparative replication dynamics and high sensitivity to thapsigargin.

Serum levels of specialised pro-resolving molecule pathways are greatly increased in SARS-CoV-2 patients and correlate with markers of the adaptive immune response (2021)
Preprint / Working Paper
Turnbull, J., Jha, R., Ortori, C. A., Lunt, E., Tighe, P. J., Irving, W. L., Gohir, S. A., Kim, D.-H., Valdes, A. M., Tarr, A. W., Barrett, D. A., & Chapman, V. Serum levels of specialised pro-resolving molecule pathways are greatly increased in SARS-CoV-2 patients and correlate with markers of the adaptive immune response

Background Specialised pro-resolution molecules (SPMs) halt the transition to chronic pathogenic inflammation. We aimed to quantify serum levels of pro- and anti-inflammatory bioactive lipids in SARS-CoV-2 patients, and to identify potential relation... Read More about Serum levels of specialised pro-resolving molecule pathways are greatly increased in SARS-CoV-2 patients and correlate with markers of the adaptive immune response.

JMM Profile: Carbapenems: a broad-spectrum antibiotic (2021)
Journal Article
Armstrong, T., Fenn, S. J., & Hardie, K. R. (2021). JMM Profile: Carbapenems: a broad-spectrum antibiotic. Journal of Medical Microbiology, 70(12), Article 001462. https://doi.org/10.1099/jmm.0.001462

Carbapenems are potent members of the β-lactam family that inhibit bacterial cell-wall biosynthesis inhibitors . They are highly effective against Gram-negative and Gram-positive drug-resistant infections . As such, carbapenems are typically reserved... Read More about JMM Profile: Carbapenems: a broad-spectrum antibiotic.

Whole-Genome Resequencing of Worldwide Wild and Domestic Sheep Elucidates Genetic Diversity, Introgression, and Agronomically Important Loci (2021)
Journal Article
Lv, F.-H., Cao, Y.-H., Liu, G.-J., Luo, L.-Y., Lu, R., Liu, M.-J., Li, W.-R., Zhou, P., Wang, X.-H., Shen, M., Gao, L., Yang, J.-Q., Yang, H., Yang, Y.-L., Liu, C.-B., Wan, P.-C., Zhang, Y.-S., Pi, W.-H., Ren, Y.-L., Shen, Z.-Q., …Li, M.-H. (2022). Whole-Genome Resequencing of Worldwide Wild and Domestic Sheep Elucidates Genetic Diversity, Introgression, and Agronomically Important Loci. Molecular Biology and Evolution, 39(2), Article msab353. https://doi.org/10.1093/molbev/msab353

Domestic sheep and their wild relatives harbor substantial genetic variants that can form the backbone of molecular breeding, but their genome landscapes remain understudied. Here, we present a comprehensive genome resource for wild ovine species, la... Read More about Whole-Genome Resequencing of Worldwide Wild and Domestic Sheep Elucidates Genetic Diversity, Introgression, and Agronomically Important Loci.

Functional modulation of PTH1R activation and signalling by RAMP2 (2021)
Preprint / Working Paper
Nemec, K., Schihada, H., Kleinau, G., Zabel, U., Grushevskyi, E. O., Scheerer, P., Lohse, M. J., & Maiellaro, I. (2021). Functional modulation of PTH1R activation and signalling by RAMP2

Receptor-activity-modifying proteins (RAMPs) are ubiquitously expressed membrane proteins that associate with different G protein-coupled receptors (GPCRs) including the parathyroid hormone 1 receptor (PTH1R), a class B GPCR and an important modulato... Read More about Functional modulation of PTH1R activation and signalling by RAMP2.

Distinct small non-coding RNA landscape in the axons and released extracellular vesicles of developing primary cortical neurons and the axoplasm of adult nerves (2021)
Journal Article
Mesquita-Ribeiro, R., Fort, R. S., Rathbone, A., Farias, J., Lucci, C., James, V., Sotelo-Silveira, J., Duhagon, M. A., & Dajas-Bailador, F. (2021). Distinct small non-coding RNA landscape in the axons and released extracellular vesicles of developing primary cortical neurons and the axoplasm of adult nerves. RNA Biology, 18(sup2), 832-855. https://doi.org/10.1080/15476286.2021.2000792

Neurons have highlighted the needs for decentralized gene expression and specific RNA function in somato-dendritic and axonal compartments, as well as in intercellular communication via extracellular vesicles (EVs). Despite advances in miRNA biology,... Read More about Distinct small non-coding RNA landscape in the axons and released extracellular vesicles of developing primary cortical neurons and the axoplasm of adult nerves.

Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function (2021)
Journal Article
Singh, S., Almuhanna, Y., Alshahrani, M. Y., Lowman, D. W., Rice, P. J., Gell, C., Ma, Z., Graves, B., Jackson, D., Lee, K., Juarez, R., Koranteng, J., Muntaka, S., Mitchell, D. A., da Silva, A. C., Hussain, F., Yilmaz, G., Mastrotto, F., Irie, Y., Williams, P., …Martinez-Pomares, L. (2021). Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function. npj Biofilms and Microbiomes, 7(1), Article 87. https://doi.org/10.1038/s41522-021-00257-w

Bacterial biofilms represent a challenge to the healthcare system because of their resilience against antimicrobials and immune attack. Biofilms consist of bacterial aggregates embedded in an extracellular polymeric substance (EPS) composed of polysa... Read More about Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function.

Immunogenicity, safety, and reactogenicity of heterologous COVID-19 primary vaccination incorporating mRNA, viral-vector, and protein-adjuvant vaccines in the UK (Com-COV2): a single-blind, randomised, phase 2, non-inferiority trial (2021)
Journal Article
Stuart, A. S. V., Shaw, R. H., Liu, X., Greenland, M., Aley, P. K., Andrews, N. J., Cameron, J. C., Charlton, S., Clutterbuck, E. A., Collins, A. M., Darton, T., Dinesh, T., Duncan, C. J. A., England, A., Faust, S. N., Ferreira, D. M., Finn, A., Goodman, A. L., Green, C. A., Hallis, B., …Snape, M. D. (2022). Immunogenicity, safety, and reactogenicity of heterologous COVID-19 primary vaccination incorporating mRNA, viral-vector, and protein-adjuvant vaccines in the UK (Com-COV2): a single-blind, randomised, phase 2, non-inferiority trial. Lancet, 399(10319), 36-49. https://doi.org/10.1016/S0140-6736%2821%2902718-5

Background: Given the importance of flexible use of different COVID-19 vaccines within the same schedule to facilitate rapid deployment, we studied mixed priming schedules incorporating an adenoviral-vectored vaccine (ChAdOx1 nCoV-19 [ChAd], AstraZen... Read More about Immunogenicity, safety, and reactogenicity of heterologous COVID-19 primary vaccination incorporating mRNA, viral-vector, and protein-adjuvant vaccines in the UK (Com-COV2): a single-blind, randomised, phase 2, non-inferiority trial.

Whole blood‐based measurement of SARS‐CoV‐2‐specific T cells reveals asymptomatic infection and vaccine immunogenicity in healthy subjects and patients with solid‐organ cancers (2021)
Journal Article
Scurr, M. J., Zelek, W. M., Lippiatt, G., Somerville, M., Burnell, S. E., Capitani, L., Davies, K., Lawton, H., Tozer, T., Rees, T., Roberts, K., Evans, M., Jackson, A., Young, C., Fairclough, L., Tighe, P., Wills, M., Westwell, A. D., Morgan, B. P., Gallimore, A., & Godkin, A. (2022). Whole blood‐based measurement of SARS‐CoV‐2‐specific T cells reveals asymptomatic infection and vaccine immunogenicity in healthy subjects and patients with solid‐organ cancers. Immunology, 165(2), 250-259. https://doi.org/10.1111/imm.13433

Accurate assessment of SARS-CoV-2 immunity is critical in evaluating vaccine efficacy and devising public health policies. Whilst the exact nature of effective immunity remains incompletely defined, SARS-CoV-2-specific T-cell responses are a critical... Read More about Whole blood‐based measurement of SARS‐CoV‐2‐specific T cells reveals asymptomatic infection and vaccine immunogenicity in healthy subjects and patients with solid‐organ cancers.