All Outputs (793)

Epen-22. Single-cell RNA sequencing identifies upregulation of IKZF1 in PFA2 myeloid subpopulation driving an anti-tumor phenotype (2020)
Presentation / Conference Contribution
Griesinger, A., Prince, E., Donson, A., Riemondy, K., Ritzman, T., Harris, F., …Foreman, N. (2020). Epen-22. Single-cell RNA sequencing identifies upregulation of IKZF1 in PFA2 myeloid subpopulation driving an anti-tumor phenotype. Neuro-Oncology, 22(Supplement 3), iii312. https://doi.org/10.1093/neuonc/noaa222.159

We have previously shown immune gene phenotype variations between posterior fossa ependymoma subgroups. PFA1 tumors chronically secrete IL-6, which pushes the infiltrating myeloid cells to an immune suppressive function. In contrast, PFA2 tumors have... Read More about Epen-22. Single-cell RNA sequencing identifies upregulation of IKZF1 in PFA2 myeloid subpopulation driving an anti-tumor phenotype.

Epen-23. A computational analysis of the tumour immune microenvironment in paediatric ependymoma (2020)
Presentation / Conference Contribution
Ritzmann, T., Lourdusamy, A., Jackson, A., Storer, L., Donson, A., Griesinger, A., …Grundy, R. (2020). Epen-23. A computational analysis of the tumour immune microenvironment in paediatric ependymoma. Neuro-Oncology, 22(Supplement 3), iii312. https://doi.org/10.1093/neuonc/noaa222.160

Ependymoma is the third commonest childhood brain tumour. Relapse is frequent, often fatal and current therapeutic strategies are inadequate. Previous ependymoma research describes an immunosuppressive environment with T-cell exhaustion, indicating a... Read More about Epen-23. A computational analysis of the tumour immune microenvironment in paediatric ependymoma.

Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease (2020)
Journal Article
Ashworth, J. C., Morgan, R. L., Lis-Slimak, K., Jones, S., Spence, K., Slater, C. E., …Merry, C. L. R. (2020). Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease. Journal of Visualized Experiments, 166, Article e61710. https://doi.org/10.3791/61710

There is a growing awareness that cells grown in 3D better model in vivo behavior than those grown in 2D. In this protocol, we describe a simple and tunable 3D hydrogel, suitable for culturing cells and tissue in a setting that matches their native e... Read More about Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease.

Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome Characterized by Developmental Delay and Corpus Callosum, Skeletal, and Renal Abnormalities (2020)
Journal Article
Schneeberger, P., von Elsner, L., Barker, E. L., Meinecke, P., Marquardt, I., Alawi, M., …Kutsche, K. (2020). Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome Characterized by Developmental Delay and Corpus Callosum, Skeletal, and Renal Abnormalities. American Journal of Human Genetics, 107(6), 1044-1061. https://doi.org/10.1016/j.ajhg.2020.10.007

Heparan sulfate belongs to the group of glycosaminoglycans (GAGs), highly sulfated linear polysaccharides. Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) is one of several specialized enzymes required for heparan sulfate synthesis and catalyzes the... Read More about Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome Characterized by Developmental Delay and Corpus Callosum, Skeletal, and Renal Abnormalities.

Ligand-directed covalent labelling of a GPCR with a fluorescent tag in live cells (2020)
Journal Article
Stoddart, L. A., Kindon, N. D., Otun, O., Harwood, C. R., Patera, F., Veprintsev, D. B., …Kellam, B. (2020). Ligand-directed covalent labelling of a GPCR with a fluorescent tag in live cells. Communications Biology, 3(1), Article 722. https://doi.org/10.1038/s42003-020-01451-w

© 2020, The Author(s). To study the localisation of G protein-coupled receptors (GPCR) in their native cellular environment requires their visualisation through fluorescent labelling. To overcome the requirement for genetic modification of the recept... Read More about Ligand-directed covalent labelling of a GPCR with a fluorescent tag in live cells.

Ran GTPase is an independent prognostic marker in malignant melanoma which promotes tumour cell migration and invasion (2020)
Journal Article
Elsheikh, S., Kouzoukakis, I., Fielden, C., Li, W., Lashin, S. E., Khair, N., …Ilyas, M. (2022). Ran GTPase is an independent prognostic marker in malignant melanoma which promotes tumour cell migration and invasion. Journal of Clinical Pathology, 75(1), 24-29. https://doi.org/10.1136/jclinpath-2020-206871

Aims Ran GTPase is involved in nucleocytoplasmic shuttling of proteins and is overexpressed in several cancers. The expression of Ran in malignant melanoma (MM) and its functional activity have not been described and were investigated in this study.... Read More about Ran GTPase is an independent prognostic marker in malignant melanoma which promotes tumour cell migration and invasion.

Patterns of biomarker expression in patients treated with primary endocrine therapy – a unique insight using core needle biopsy tissue microarray (2020)
Journal Article
Parks, R. M., Albanghali, M. A., Syed, B. M., Green, A. R., Ellis, I. O., & Cheung, K. L. (2021). Patterns of biomarker expression in patients treated with primary endocrine therapy – a unique insight using core needle biopsy tissue microarray. Breast Cancer Research and Treatment, 185(3), 647-655. https://doi.org/10.1007/s10549-020-06023-4

© 2020, The Author(s). Purpose: Prediction of response to primary endocrine therapy (PET) in older women is based on measurement of oestrogen receptor (ER), progesterone receptor (PgR) and human epidermal growth factor (HER)-2. This study uses a uniq... Read More about Patterns of biomarker expression in patients treated with primary endocrine therapy – a unique insight using core needle biopsy tissue microarray.

A same‐day assay predicts apoptotic response to combined BCL‐2 and MCL‐1 BH3‐mimetic targeting in multiple myeloma cells (2020)
Journal Article
Grundy, M., Al‐Kaisi, F., Cull, J., Williams, C., Smith, D., & Seedhouse, C. H. (2021). A same‐day assay predicts apoptotic response to combined BCL‐2 and MCL‐1 BH3‐mimetic targeting in multiple myeloma cells. eJHaem, 2(1), 40-47. https://doi.org/10.1002/jha2.133

Recent advances in treatment options for multiple myeloma (MM) have positive impact on patient survival. However, there is a short fall of rapid and reliable assays that can predict patient response to novel agents. The anti-apoptotic proteins B-cell... Read More about A same‐day assay predicts apoptotic response to combined BCL‐2 and MCL‐1 BH3‐mimetic targeting in multiple myeloma cells.

3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome (2020)
Journal Article
Linke, F., Aldighieri, M., Lourdusamy, A., Grabowska, A. M., Stolnik, S., Kerr, I. D., …Coyle, B. (2021). 3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome. Journal of Pathology, 253(3), 326-338. https://doi.org/10.1002/path.5591

© 2020 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland. Medulloblastoma (MB) is the most common malignant brain tumour in children and is subdivided into... Read More about 3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome.

Advance care planning in patients with advanced cancer: A 6-country, cluster-randomised clinical trial (2020)
Journal Article
Korfage, I. J., Carreras, G., Arnfeldt Christensen, C. M., Billekens, P., Bramley, L., Briggs, L., …Rietjens, J. A. C. (2020). Advance care planning in patients with advanced cancer: A 6-country, cluster-randomised clinical trial. PLoS Medicine, 17(11), Article e1003422. https://doi.org/10.1371/journal.pmed.1003422

Background: Advance care planning (ACP) supportsindividuals to define, discuss, and record goals and preferences for future medical treatment and care. Despite being internationally recommended, randomized clinical trials of ACP in patients with adva... Read More about Advance care planning in patients with advanced cancer: A 6-country, cluster-randomised clinical trial.

AKT isoforms have discrete expression in Triple Negative breast cancers and roles in cisplatin sensitivity (2020)
Journal Article
Khan, S., Wadhwa, B., Paddar, M., Malik, F., Mir, S., Clarke, P. A., …Malik, F. (2020). AKT isoforms have discrete expression in Triple Negative breast cancers and roles in cisplatin sensitivity. Oncotarget, 11, 4178-4194. https://doi.org/10.18632/oncotarget.27746

AKT, a serine threonine kinase, exists in three different isoforms and is known for regulating several biological processes including tumorigenesis. In this study, we investigated the expression and net effect of the individual isoforms in triple neg... Read More about AKT isoforms have discrete expression in Triple Negative breast cancers and roles in cisplatin sensitivity.

Nucleolar protein 10 (NOP10) predicts poor prognosis in invasive breast cancer (2020)
Journal Article
Elsharawy, K. A., Althobiti, M., Mohammed, O. J., Aljohani, A. I., Toss, M. S., Green, A. R., & Rakha, E. (2021). Nucleolar protein 10 (NOP10) predicts poor prognosis in invasive breast cancer. Breast Cancer Research and Treatment, 185(3), 615-627. https://doi.org/10.1007/s10549-020-05999-3

Purpose
Nucleolar protein 10 (NOP10) is required for ribosome biogenesis and telomere maintenance and plays a key role in carcinogenesis. This study aims to evaluate the clinical and prognostic significance of NOP10 in breast cancer (BC).

Methods... Read More about Nucleolar protein 10 (NOP10) predicts poor prognosis in invasive breast cancer.

Annual mammographic screening to reduce breast cancer mortality in women from age 40 years: long-term follow-up of the uk age rct (2020)
Journal Article
Duffy, S., Vulkan, D., Cuckle, H., Parmar, D., Sheikh, S., Smith, R., …Moss, S. (2020). Annual mammographic screening to reduce breast cancer mortality in women from age 40 years: long-term follow-up of the uk age rct. Health Technology Assessment, 24(55), 1-24. https://doi.org/10.3310/hta24550

Background There remains disagreement on the long-term effect of mammographic screening in women aged 40-49 years. Objectives The long-term follow-up of a randomised controlled trial that offered annual mammography to women aged 40-49 years. The esti... Read More about Annual mammographic screening to reduce breast cancer mortality in women from age 40 years: long-term follow-up of the uk age rct.

Markers of steroid receptor, kinase signalling pathways and Ki-67 expression in relation to tamoxifen sensitivity and resistance (2020)
Journal Article
Campbell, C., Mathew, J., Ellis, I. O., Bradbury, I., Borgquist, S., Elebro, K., …Robertson, J. F. R. (2020). Markers of steroid receptor, kinase signalling pathways and Ki-67 expression in relation to tamoxifen sensitivity and resistance. Translational Breast Cancer Research, 1, Article 29. https://doi.org/10.21037/tbcr-20-31

Background: It remains clinically important to identify ER positive breast cancers likely to respond to tamoxifen (TAM) and so we aimed to select a group of biomarkers able to predict response. We also assessed whether data from different sample type... Read More about Markers of steroid receptor, kinase signalling pathways and Ki-67 expression in relation to tamoxifen sensitivity and resistance.

Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells (2020)
Journal Article
Pérez-Cota, F., Fuentes-Domínguez, R., La Cavera III, S., Hardiman, W., Yao, M., Setchfield, K., …Clark, M. (2020). Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells. Journal of Applied Physics, 128(16), Article 160902. https://doi.org/10.1063/5.0023744

© 2020 Author(s). Characterization of the elasticity of biological cells is growing as a new way to gain insight into cell biology. Cell mechanics are related to most aspects of cellular behavior, and applications in research and medicine are broad.... Read More about Picosecond ultrasonics for elasticity-based imaging and characterization of biological cells.

CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes (2020)
Journal Article
Kondrashov, A., Mohd Yusof, N. A., Hasan, A., Goulding, J., Kodagoda, T., Hoang, D. M., …Denning, C. (2021). CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes. Molecular Therapy - Methods and Clinical Development, 20, 39-53. https://doi.org/10.1016/j.omtm.2020.10.019

© 2020 During normal- and patho-physiological situations, the behavior of the beta2-adrenoreceptor (β2AR) is influenced by polymorphic variants. The functional impact of such polymorphisms has been suggested from data derived from genetic association... Read More about CRISPR/Cas9-mediated generation and analysis of N terminus polymorphic models of β2AR in isogenic hPSC-derived cardiomyocytes.

Scedosporium apiospermum brain abscesses in a patient receiving ibrutinib and venetoclax (2020)
Journal Article
Figueroa, R., Martinez‐Calle, N., Prescott, K., & Bishton, M. (2020). Scedosporium apiospermum brain abscesses in a patient receiving ibrutinib and venetoclax. eJHaem, 1(2), 418-419. https://doi.org/10.1002/jha2.121

An 80-year-old male, with relapsed mantle cell lymphoma was treated on a trial with the combination of the bruton tyrosine kinase (BTK) inhibitor, ibrutinib, and BH3-mimetic, venetoclax, and achieved complete remission by both Computerized Tomography... Read More about Scedosporium apiospermum brain abscesses in a patient receiving ibrutinib and venetoclax.

Effect of varying the Mg with Ca content in highly porous phosphate-based glass microspheres (2020)
Journal Article
Islam, M. T., Macri-Pellizzeri, L., Hossain, K. M. Z., Sottile, V., & Ahmed, I. (2021). Effect of varying the Mg with Ca content in highly porous phosphate-based glass microspheres. Materials Science and Engineering: C, 120, Article 111668. https://doi.org/10.1016/j.msec.2020.111668

Natural ventilation is a low energy strategy used in many building types. Design approaches are mature but are dependent on variables with high uncertainty, such as the aerodynamic behaviour of purpose provided openings (PPOs), which need improved ch... Read More about Effect of varying the Mg with Ca content in highly porous phosphate-based glass microspheres.

The prognostic significance of Interferon Stimulated Gene 15 (ISG15) in invasive breast cancer (2020)
Journal Article
Kariri, Y., Alsaleem, M., Joseph, C., Alsaeed, S., Aljohani, A., Shiino, S., …Rakha, E. A. (2021). The prognostic significance of Interferon Stimulated Gene 15 (ISG15) in invasive breast cancer. Breast Cancer Research and Treatment, 185(2), 293–305. https://doi.org/10.1007/s10549-020-05955-1

Background

Lymphovascular invasion (LVI) is a prognostic factor in early-stage invasive breast cancer (BC). Through bioinformatics, data analyses of multiple BC cohorts revealed the positive association between interferon-stimulated gene 15 (ISG15... Read More about The prognostic significance of Interferon Stimulated Gene 15 (ISG15) in invasive breast cancer.

Effects of Polymer 3D Architecture, Size, and Chemistry on Biological Transport and Drug Delivery In Vitro and in Orthotopic Triple Negative Breast Cancer Models (2020)
Journal Article
Anane‐Adjei, A. B., Pearce, A. K., Anane-Adjei, A. B., Cavanagh, R. J., Monteiro, P. F., Bennett, T. M., …Alexander, C. (2020). Effects of Polymer 3D Architecture, Size, and Chemistry on Biological Transport and Drug Delivery In Vitro and in Orthotopic Triple Negative Breast Cancer Models. Advanced Healthcare Materials, 9(22), Article 2000892. https://doi.org/10.1002/adhm.202000892

© 2020 Wiley-VCH GmbH The size, shape, and underlying chemistries of drug delivery particles are key parameters which govern their ultimate performance in vivo. Responsive particles are desirable for triggered drug delivery, achievable through archit... Read More about Effects of Polymer 3D Architecture, Size, and Chemistry on Biological Transport and Drug Delivery In Vitro and in Orthotopic Triple Negative Breast Cancer Models.