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Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease

Ashworth, J C; Morgan, R L; Lis-Slimak, K; Jones, S; Spence, K; Slater, C E; Thompson, J L; Grabowska, A M; Clarke, R B; Farnie, G; Merry, C L R

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Authors

R L Morgan

K Lis-Slimak

S Jones

K Spence

C E Slater

J L Thompson

A M Grabowska

R B Clarke

G Farnie

Profile image of CATHY MERRY

CATHY MERRY Cathy.Merry@nottingham.ac.uk
Professor of Stem Glycobiology



Abstract

There is a growing awareness that cells grown in 3D better model in vivo behavior than those grown in 2D. In this protocol, we describe a simple and tunable 3D hydrogel, suitable for culturing cells and tissue in a setting that matches their native environment. This is particularly important for researchers investigating the initiation, growth, and treatment of cancer where the interaction between cells and their local extracellular matrix is a fundamental part of the model.

Citation

Ashworth, J. C., Morgan, R. L., Lis-Slimak, K., Jones, S., Spence, K., Slater, C. E., …Merry, C. L. R. (2020). Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease. Journal of Visualized Experiments, 166, Article e61710. https://doi.org/10.3791/61710

Journal Article Type Article
Acceptance Date Sep 2, 2020
Online Publication Date Dec 3, 2020
Publication Date Dec 3, 2020
Deposit Date Sep 5, 2020
Publicly Available Date Jun 4, 2021
Journal Journal of Visualized Experiments
Electronic ISSN 1940-087X
Publisher Journal of Visualized Experiments
Peer Reviewed Peer Reviewed
Volume 166
Article Number e61710
DOI https://doi.org/10.3791/61710
Public URL https://nottingham-repository.worktribe.com/output/4886223
Publisher URL https://www.jove.com/t/61710/preparation-user-defined-peptide-gel-for-controlled-3d-culture-models

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