Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease

Ashworth, J C; Morgan, R L; Lis-Slimak, K; Jones, S; Spence, K; Slater, C E; Thompson, J L; Grabowska, A M; Clarke, R B; Farnie, G; Merry, C L R

Abstract

There is a growing awareness that cells grown in 3D better model in vivo behavior than those grown in 2D. In this protocol, we describe a simple and tunable 3D hydrogel, suitable for culturing cells and tissue in a setting that matches their native environment. This is particularly important for researchers investigating the initiation, growth, and treatment of cancer where the interaction between cells and their local extracellular matrix is a fundamental part of the model.

Citation

Ashworth, J. C., Morgan, R. L., Lis-Slimak, K., Jones, S., Spence, K., Slater, C. E., Thompson, J. L., Grabowska, A. M., Clarke, R. B., Farnie, G., & Merry, C. L. R. (2020). Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease. Journal of Visualized Experiments, 166, Article e61710. https://doi.org/10.3791/61710

• Journal Article Type: Article
• Acceptance Date: Sep 2, 2020
• Online Publication Date: Dec 3, 2020
• Publication Date: Dec 3, 2020
• Deposit Date: Sep 5, 2020
• Publicly Available Date: Jun 4, 2021
• Journal: Journal of Visualized Experiments
• Electronic ISSN: 1940-087X
• Publisher: Journal of Visualized Experiments
• Peer Reviewed: Peer Reviewed
• Volume: 166
• Article Number: e61710
• DOI: https://doi.org/10.3791/61710
• Public URL: https://nottingham-repository.worktribe.com/output/4886223
• Publisher URL: https://www.jove.com/t/61710/preparation-user-defined-peptide-gel-for-controlled-3d-culture-models