All Outputs (2303)

Metabolomics as an Important Tool for Determining the Mechanisms of Human Skeletal Muscle Deconditioning (2021)
Journal Article
Alldritt, I., Greenhaff, P. L., & Wilkinson, D. J. (2021). Metabolomics as an Important Tool for Determining the Mechanisms of Human Skeletal Muscle Deconditioning. International Journal of Molecular Sciences, 22(24), Article 13575. https://doi.org/10.3390/ijms222413575

Muscle deconditioning impairs both locomotor function and metabolic health, and is associated with reduced quality life and increased mortality rates. Despite an appreciation of the existence of phenomena such as muscle anabolic resistance, mitophagy... Read More about Metabolomics as an Important Tool for Determining the Mechanisms of Human Skeletal Muscle Deconditioning.

Application of microfluidic systems in modelling impacts of environmental structure on stress-sensing by individual microbial cells (2021)
Journal Article
Harvey, H. J., Chubynsky, M. V., Sprittles, J. E., Shor, L. M., Mooney, S. J., Wildman, R. D., & Avery, S. V. (2022). Application of microfluidic systems in modelling impacts of environmental structure on stress-sensing by individual microbial cells. Computational and Structural Biotechnology Journal, 20, 128-138. https://doi.org/10.1016/j.csbj.2021.11.039

Environmental structure describes physical structure that can determine heterogenous spatial distribution of biotic and abiotic (nutrients, stressors etc.) components of a microorganism's microenvironment. This study investigated the impact of microm... Read More about Application of microfluidic systems in modelling impacts of environmental structure on stress-sensing by individual microbial cells.

Adaptive Evolution in TRIF Leads to Discordance between Human and Mouse Innate Immune Signaling (2021)
Journal Article
Hyland, E. M., Webb, A. E., Kennedy, K. F., Gerek Ince, Z. N., Loscher, C. E., & O'Connell, M. J. (2021). Adaptive Evolution in TRIF Leads to Discordance between Human and Mouse Innate Immune Signaling. Genome Biology and Evolution, 13(12), Article evab268. https://doi.org/10.1093/gbe/evab268

The TIR domain-containing adapter inducing IFN-β (TRIF) protein is an innate immune system protein that mediates the MyD88-independent toll-like receptor response pathway in mice and humans. Previously, we identified positive selection at seven disti... Read More about Adaptive Evolution in TRIF Leads to Discordance between Human and Mouse Innate Immune Signaling.

Evolutionary footprints of a cold relic in a rapidly warming world (2021)
Journal Article
Wolf, E., Gaquerel, E., Scharmann, M., Yant, L., & Koch, M. A. (2021). Evolutionary footprints of a cold relic in a rapidly warming world. eLife, 10, Article e71572. https://doi.org/10.7554/elife.71572

With accelerating global warming, understanding the evolutionary dynamics of plant adaptation to environmental change is increasingly urgent. Here we reveal the enigmatic history of the genus Cochlearia (Brassicaceae), a Pleistocene relic that origin... Read More about Evolutionary footprints of a cold relic in a rapidly warming world.

Mimicking native display of cd0873 on liposomes augments its potency as an oral vaccine against clostridioides difficile (2021)
Journal Article
Karyal, C., Palazi, P., Hughes, J., Griffiths, R. C., Persaud, R. R., Tighe, P. J., …Griffin, R. (2021). Mimicking native display of cd0873 on liposomes augments its potency as an oral vaccine against clostridioides difficile. Vaccines, 9(12), Article 1453. https://doi.org/10.3390/vaccines9121453

Mucosal vaccination aims to prevent infection mainly by inducing secretory IgA (sIgA) antibody, which neutralises pathogens and enterotoxins by blocking their attachment to epithelial cells. We previously demonstrated that encapsulated protein antige... Read More about Mimicking native display of cd0873 on liposomes augments its potency as an oral vaccine against clostridioides difficile.

Solving sampling bias problems in presence–absence or presence-only species data using zero-inflated models (2021)
Journal Article
Nolan, V., Gilbert, F., & Reader, T. (2022). Solving sampling bias problems in presence–absence or presence-only species data using zero-inflated models. Journal of Biogeography, 49(1), 215-232. https://doi.org/10.1111/jbi.14268

Aim: Large databases of species records such as those generated through citizen science projects, archives or museum collections are being used with increasing frequency in species distribution modelling (SDM) for conservation and land management. De... Read More about Solving sampling bias problems in presence–absence or presence-only species data using zero-inflated models.

Versatile selective evolutionary pressure using synthetic defect in universal metabolism (2021)
Journal Article
Sellés Vidal, L., Murray, J. W., & Heap, J. T. (2021). Versatile selective evolutionary pressure using synthetic defect in universal metabolism. Nature Communications, 12(1), Article 6859. https://doi.org/10.1038/s41467-021-27266-9

The non-natural needs of industrial applications often require new or improved enzymes. The structures and properties of enzymes are difficult to predict or design de novo. Instead, semi-rational approaches mimicking evolution entail diversification... Read More about Versatile selective evolutionary pressure using synthetic defect in universal metabolism.

The maintenance of standing genetic variation: Gene flow vs. selective neutrality in Atlantic stickleback fish (2021)
Journal Article
Haenel, Q., Guerard, L., MacColl, A. D. C., & Berner, D. (2022). The maintenance of standing genetic variation: Gene flow vs. selective neutrality in Atlantic stickleback fish. Molecular Ecology, 31(3), 811-821. https://doi.org/10.1111/mec.16269

Adaptation to derived habitats often occurs from standing genetic variation. The maintenance within ancestral populations of genetic variants favourable in derived habitats is commonly ascribed to long-term antagonism between purifying selection and... Read More about The maintenance of standing genetic variation: Gene flow vs. selective neutrality in Atlantic stickleback fish.

Structures of the Human SPAK and OSR1 Conserved C-Terminal (CCT) Domains (2021)
Journal Article
Elvers, K. T., Lipka-Lloyd, M., Trueman, R. C., Bax, B. D., & Mehellou, Y. (2022). Structures of the Human SPAK and OSR1 Conserved C-Terminal (CCT) Domains. ChemBioChem, 23(1), Article e202100441. https://doi.org/10.1002/cbic.202100441

STE20/SPS1-related proline/alanine-rich kinase (SPAK) and oxidative stress responsive 1 (OSR1) kinase are two serine/threonine protein kinases that regulate the function of ion co-transporters through phosphorylation. The highly conserved C-terminal... Read More about Structures of the Human SPAK and OSR1 Conserved C-Terminal (CCT) Domains.

Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo (2021)
Journal Article
James, V., Nizamudeen, Z. A., Lea, D., Dottorini, T., Holmes, T. L., Johnson, B. B., …Smith, J. G. (2021). Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo. Stem Cells and Development, 30(24), 1215-1227. https://doi.org/10.1089/scd.2021.0202

Hypertrophic cardiomyopathy (HCM) is characterised by increased left ventricular wall thickness that can lead to devastating conditions such as heart failure and sudden cardiac death. Despite extensive study, the mechanisms mediating many of the asso... Read More about Transcriptomic Analysis of Cardiomyocyte Extracellular Vesicles in Hypertrophic Cardiomyopathy Reveals Differential snoRNA Cargo.

Poroelastic osmoregulation of living cell volume (2021)
Journal Article
Esteki, M. H., Malandrino, A., Alemrajabi, A. A., Sheridan, G. K., Charras, G., & Moeendarbary, E. (2021). Poroelastic osmoregulation of living cell volume. iScience, 24(12), Article 103482. https://doi.org/10.1016/j.isci.2021.103482

Cells maintain their volume through fine intracellular osmolarity regulation. Osmotic challenges drive fluid into or out of cells causing swelling or shrinkage, respectively. The dynamics of cell volume changes depending on the rheology of the cellul... Read More about Poroelastic osmoregulation of living cell volume.

Target-group backgrounds prove effective at correcting sampling bias in Maxent models (2021)
Journal Article
Barber, R. A., Ball, S. G., Morris, R. K., & Gilbert, F. (2022). Target-group backgrounds prove effective at correcting sampling bias in Maxent models. Diversity and Distributions, 28(1), 128-141. https://doi.org/10.1111/ddi.13442

Aim: Accounting for sampling bias is the greatest challenge facing presence-only and presence-background species distribution models; no matter what type of model is chosen, using biased data will mask the true relationship between occurrences and en... Read More about Target-group backgrounds prove effective at correcting sampling bias in Maxent models.

Quantitative assessment of sitting time in ambulant adults with Muscular Dystrophy (2021)
Journal Article
Jacques, M. F., Onambele-Pearson, G. L., Edwards, B., De Goede, C. G., & Morse, C. I. (2021). Quantitative assessment of sitting time in ambulant adults with Muscular Dystrophy. PLoS ONE, 16(11), Article e0260491. https://doi.org/10.1371/journal.pone.0260491

Background Current investigations into physical behaviour in Muscular Dystrophy (MD) have focussed largely on physical activity (PA). Negative health behaviours such as sedentary behaviour (Physical Behaviour) and sitting time (Posture Classificatio... Read More about Quantitative assessment of sitting time in ambulant adults with Muscular Dystrophy.

Feasibility RCT of neuromuscular electrical stimulation; an Intervention to Maintain and improve neuroMuscular function during periods of Immobility (IMMI): Protocol (2021)
Preprint / Working Paper
Gladman, J., Aloraibi, S., Greenhaff, P., Piasecki, M., Phillips, B., Atherton, P., …Gordon, A. Feasibility RCT of neuromuscular electrical stimulation; an Intervention to Maintain and improve neuroMuscular function during periods of Immobility (IMMI): Protocol

East Midlands Research into Ageing Network (EMRAN) is a research collaboration across the East Midlands to facilitate collaborative applied clinical research into ageing and the care of older people. EMRAN was set up by NIHR CLAHRC East Midlands and... Read More about Feasibility RCT of neuromuscular electrical stimulation; an Intervention to Maintain and improve neuroMuscular function during periods of Immobility (IMMI): Protocol.

The anti-inflammatory effect of bacterial short chain fatty acids is partially mediated by endocannabinoids (2021)
Journal Article
Vijay, A., Kouraki, A., Gohir, S., Turnbull, J., Kelly, A., Chapman, V., …Valdes, A. M. (2021). The anti-inflammatory effect of bacterial short chain fatty acids is partially mediated by endocannabinoids. Gut Microbes, 13(1), Article 1997559. https://doi.org/10.1080/19490976.2021.1997559

The endocannabinoid (EC) system has pleiotropic functions in the body. It plays a key role in energy homeostasis and the development of metabolic disorders being a mediator in the relationship between the gut microbiota and host metabolism. In the cu... Read More about The anti-inflammatory effect of bacterial short chain fatty acids is partially mediated by endocannabinoids.

The Impact of Real-Time Whole-Genome Sequencing in Controlling Healthcare-Associated SARS-CoV-2 Outbreaks (2021)
Journal Article
Francis, R. V., Billam, H., Clarke, M., Yates, C., Tsoleridis, T., Berry, L., …McClure, C. P. (2022). The Impact of Real-Time Whole-Genome Sequencing in Controlling Healthcare-Associated SARS-CoV-2 Outbreaks. Journal of Infectious Diseases, 225(1), 10-18. https://doi.org/10.1093/infdis/jiab483

Nosocomial severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections have severely affected bed capacity and patient flow. We utilized whole-genome sequencing (WGS) to identify outbreaks and focus infection control resources and interve... Read More about The Impact of Real-Time Whole-Genome Sequencing in Controlling Healthcare-Associated SARS-CoV-2 Outbreaks.

minoTour, real-time monitoring and analysis for nanopore sequencers (2021)
Journal Article
Munro, R., Santos, R., Payne, A., Forey, T., Osei, S., Holmes, N., & Loose, M. (2022). minoTour, real-time monitoring and analysis for nanopore sequencers. Bioinformatics, 38(4), 1133-1135. https://doi.org/10.1093/bioinformatics/btab780

Summary: MinoTour offers a LIMS system for Oxford Nanopore Technology sequencers, with real-time metrics and analysis available permanently for review. Integration of unique real-time automated analysis can reduce the time required to answer biologic... Read More about minoTour, real-time monitoring and analysis for nanopore sequencers.

Immunocompromised children and young people are at no increased risk of severe COVID-19 (2021)
Journal Article
Chappell, H., Patel, R., Driessens, C., Tarr, A. W., Irving, W. L., Tighe, P. J., …de Graaf, H. (2022). Immunocompromised children and young people are at no increased risk of severe COVID-19. Journal of Infection, 84(1), 31-39. https://doi.org/10.1016/j.jinf.2021.11.005

Objectives: We aimed to prospectively describe the incidence and clinical spectrum of SARS-CoV-2 infection in immunocompromised paediatric patients in the UK. Methods: From March 2020 to 2021 weekly questionnaires were sent to immunocompromised paedi... Read More about Immunocompromised children and young people are at no increased risk of severe COVID-19.

Biosynthesis of Poly(3HB- co-3HP) with Variable Monomer Composition in Recombinant Cupriavidus necator H16 (2021)
Journal Article
McGregor, C., Minton, N. P., & Kovács, K. (2021). Biosynthesis of Poly(3HB- co-3HP) with Variable Monomer Composition in Recombinant Cupriavidus necator H16. ACS Synthetic Biology, 10(12), 3343-3352. https://doi.org/10.1021/acssynbio.1c00283

Polyhydroxyalkanoates are attractive alternatives to traditional plastics. However, although polyhydroxybutyrate (PHB) is produced in large quantities by Cupriavidus necator H16, its properties are far from ideal for the manufacture of plastic produc... Read More about Biosynthesis of Poly(3HB- co-3HP) with Variable Monomer Composition in Recombinant Cupriavidus necator H16.

Positive allosteric modulation of endogenous delta opioid receptor signaling in the enteric nervous system is a potential treatment for gastrointestinal motility disorders (2021)
Journal Article
DiCello, J. J., Carbone, S. E., Saito, A., Pham, V., Szymaszkiewicz, A., Gondin, A. B., …Poole, D. P. (2022). Positive allosteric modulation of endogenous delta opioid receptor signaling in the enteric nervous system is a potential treatment for gastrointestinal motility disorders. AJP - Gastrointestinal and Liver Physiology, 322(1), G66-G78. https://doi.org/10.1152/AJPGI.00297.2021

Allosteric modulators (AMs) are molecules that can fine-tune signaling by G protein-coupled receptors (GPCRs). Although they are a promising therapeutic approach for treating a range of disorders, allosteric modulation of GPCRs in the context of the... Read More about Positive allosteric modulation of endogenous delta opioid receptor signaling in the enteric nervous system is a potential treatment for gastrointestinal motility disorders.