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Biased Gs versus Gq proteins and β-arrestin signaling in the NK1 receptor determined by interactions in the water hydrogen bond network (2015)
Journal Article
Valentin-Hansen, L., Frimurer, T. M., Mokrosinski, J., Holliday, N. D., & Schwartz, T. W. (2015). Biased Gs versus Gq proteins and β-arrestin signaling in the NK1 receptor determined by interactions in the water hydrogen bond network. Journal of Biological Chemistry, 290(40), 24495-24508. https://doi.org/10.1074/jbc.m115.641944

X-ray structures, molecular dynamics simulations, and mutational analysis have previously indicated that an extended water hydrogen bond network between trans-membranes I-III, VI, and VII constitutes an allosteric interface essential for stabilizing... Read More about Biased Gs versus Gq proteins and β-arrestin signaling in the NK1 receptor determined by interactions in the water hydrogen bond network.

Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor (2015)
Journal Article
Strillinger, E., Grötzinger, S. W., Allers, T., Eppinger, J., & Weuster-Botz, D. (2016). Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor. Applied Microbiology and Biotechnology, 100(3), 1183-1195. https://doi.org/10.1007/s00253-015-7007-1

The success of biotechnological processes is based on the availability of efficient and highly specific biocatalysts, which can satisfy industrial demands. Extreme and remote environments like the deep brine pools of the Red Sea represent highly inte... Read More about Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor.

A structure-activity relationship study of the positive allosteric modulator LY2033298 at the M4 muscarinic acetylcholine receptor (2015)
Journal Article
Szabo, M., Huynh, T., Valant, C., Lane, J. R., Sexton, P. M., Christopoulos, A., & Capuano, B. (2015). A structure-activity relationship study of the positive allosteric modulator LY2033298 at the M4 muscarinic acetylcholine receptor. MedChemComm, 6(11), 1998-2003. https://doi.org/10.1039/C5MD00334B

Positive allosteric modulators (PAMs) targeting the M4 muscarinic acetylcholine receptor (mAChR) offer greater sub-type selectivity and unique potential as central nervous system agents through their novel mode of action to traditional orthosteric li... Read More about A structure-activity relationship study of the positive allosteric modulator LY2033298 at the M4 muscarinic acetylcholine receptor.

Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium (2015)
Journal Article
Freschi, L., Jeukens, J., Kukavica-Ibrulj, I., Boyle, B., Dupont, M.-J., Laroche, J., …Levesque, R. C. (in press). Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium. Frontiers in Microbiology, 6(1036), https://doi.org/10.3389/fmicb.2015.01036

The International Pseudomonas aeruginosa Consortium is sequencing over 1000 genomes and building an analysis pipeline for the study of Pseudomonas genome evolution, antibiotic resistance and virulence genes. Metadata, including genomic and phenotypic... Read More about Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium.

Understanding how porosity gradients can make a better filter using homogenization theory (2015)
Journal Article
Dalwadi, M. P., Griffiths, I. M., & Bruna, M. (2015). Understanding how porosity gradients can make a better filter using homogenization theory. Proceedings of the Royal Society A: Mathematical, Physical and Engineering Sciences, 471(2182), https://doi.org/10.1098/rspa.2015.0464

Filters whose porosity decreases with depth are often more efficient at removing solute from a fluid than filters with a uniform porosity. We investigate this phenomenon via an extension of homogenization theory that accounts for a macroscale variati... Read More about Understanding how porosity gradients can make a better filter using homogenization theory.

Impact of p38 mitogen-activated protein kinase inhibition on immunostimulatory properties of human 6-sulfo LacNAc dendritic cells (2015)
Journal Article
Langosch, S., Wehner, R., Malecka, A., Franks, H. A., Schäkel, K., Bachmann, M., …Schmitz, M. (2016). Impact of p38 mitogen-activated protein kinase inhibition on immunostimulatory properties of human 6-sulfo LacNAc dendritic cells. Immunobiology, 221(2), 166-174. https://doi.org/10.1016/j.imbio.2015.09.012

p38 Mitogen-activated protein kinase (MAPK) plays a crucial role in the induction and regulation of innate and adaptive immunity. Furthermore, p38 MAPK can promote tumor invasion, metastasis, and angiogenesis. Based on these properties, p38 MAPK inhi... Read More about Impact of p38 mitogen-activated protein kinase inhibition on immunostimulatory properties of human 6-sulfo LacNAc dendritic cells.

Conflict of interest and signal interference lead to the breakdown of honest signalling (2015)
Journal Article
Popat, R., Pollitt, E. J., Harrison, F., Naghra, H., Hong, K. W., Chan, K. G., …Diggle, S. P. (2015). Conflict of interest and signal interference lead to the breakdown of honest signalling. Evolution, 69(9), https://doi.org/10.1111/evo.12751

Animals use signals to coordinate a wide range of behaviours, from feeding offspring to predator avoidance. This poses an evolutionary problem, because individuals could potentially signal dishonestly to coerce others into behaving in ways that benef... Read More about Conflict of interest and signal interference lead to the breakdown of honest signalling.

Effect of extended morning fasting upon ad libitum lunch intake and associated metabolic and hormonal responses in obese adults (2015)
Journal Article
Chowdhury, E., Richardson, J., Tsintzas, K., Thompson, D., & Betts, J. (in press). Effect of extended morning fasting upon ad libitum lunch intake and associated metabolic and hormonal responses in obese adults. International Journal of Obesity, 40(2), https://doi.org/10.1038/ijo.2015.154

Background/Objectives: Breakfast omission is positively associated with obesity and increased risk of disease. However, little is known about the acute effects of extended morning fasting upon subsequent energy intake and associated metabolic/reg... Read More about Effect of extended morning fasting upon ad libitum lunch intake and associated metabolic and hormonal responses in obese adults.

Obesity appears to be associated with altered muscle protein synthetic and breakdown responses to increased nutrient delivery in older men, but not reduced muscle mass or contractile function. (2015)
Journal Article
Murton, A. J., Marimuthu, K., Mallinson, J. E., Selby, A. L., Smith, K., Rennie, M. J., & Greenhaff, P. L. (2015). Obesity appears to be associated with altered muscle protein synthetic and breakdown responses to increased nutrient delivery in older men, but not reduced muscle mass or contractile function. Diabetes, 64(9), https://doi.org/10.2337/db15-0021

Obesity is increasing, yet despite the necessity to maintain muscle mass and function with age, the effect of obesity on muscle protein turnover in older adults remains unknown. Eleven obese (BMI 31.9 ±1.1) and 15 healthy weight (HW; BMI 23.4 ±0.3) o... Read More about Obesity appears to be associated with altered muscle protein synthetic and breakdown responses to increased nutrient delivery in older men, but not reduced muscle mass or contractile function..

Pregnancy-related changes in the maternal gut microbiota are dependent upon the mother's periconceptional diet (2015)
Journal Article
Gohir, W., Whelan, F. J., Surette, M. G., Moore, C., Schertzer, J. D., & Sloboda, D. M. (2015). Pregnancy-related changes in the maternal gut microbiota are dependent upon the mother's periconceptional diet. Gut Microbes, 6(5), 310-320. https://doi.org/10.1080/19490976.2015.1086056

Shifts in the maternal gut microbiome have been implicated in metabolic adaptations to pregnancy. We investigated how pregnancy and diet interact to influence the composition of the maternal gut microbiota. Female C57BL/6 mice were fed either a contr... Read More about Pregnancy-related changes in the maternal gut microbiota are dependent upon the mother's periconceptional diet.

Discovery of a Novel Class of Negative Allosteric Modulator of the Dopamine D2 Receptor Through Fragmentation of a Bitopic Ligand (2015)
Journal Article
Mistry, S. N., Shonberg, J., Draper-Joyce, C. J., Klein Herenbrink, C., Michino, M., Shi, L., …Lane, J. R. (2015). Discovery of a Novel Class of Negative Allosteric Modulator of the Dopamine D2 Receptor Through Fragmentation of a Bitopic Ligand. Journal of Medicinal Chemistry, 58(17), 6819-6843. https://doi.org/10.1021/acs.jmedchem.5b00585

We recently demonstrated that SB269652 (1) engages one protomer of a dopamine D2 receptor (D2R) dimer in a bitopic mode to allosterically inhibit the binding of dopamine at the other protomer. Herein, we investigate structural deter- minants for allo... Read More about Discovery of a Novel Class of Negative Allosteric Modulator of the Dopamine D2 Receptor Through Fragmentation of a Bitopic Ligand.

Targeting a host-cell entry factor barricades antiviral-resistant HCV variants from on-therapy breakthrough in human-liver mice (2015)
Journal Article
Vercauteren, K., Brown, R. J., Mesalam, A. A., Doerrbecker, J., Bhuju, S., Geffers, R., …Meuleman, P. (2016). Targeting a host-cell entry factor barricades antiviral-resistant HCV variants from on-therapy breakthrough in human-liver mice. Gut, 65(12), https://doi.org/10.1136/gutjnl-2014-309045

Objective: Direct-acting antivirals (DAAs) inhibit hepatitis C virus (HCV) infection by targeting viral proteins that play essential roles in the replication process. However, selection of resistance-associated variants (RAVs) during DAA therapy has... Read More about Targeting a host-cell entry factor barricades antiviral-resistant HCV variants from on-therapy breakthrough in human-liver mice.

Identification of residues in ABCG2 affecting protein trafficking and drug transport, using co-evolutionary analysis of ABCG sequences (2015)
Journal Article

ABCG2 is an ABC (ATP-binding cassette) transporter with a physiological role in urate transport in the kidney and is also implicated in multi-drug efflux from a number of organs in the body. The trafficking of the protein and the mechanism by which i... Read More about Identification of residues in ABCG2 affecting protein trafficking and drug transport, using co-evolutionary analysis of ABCG sequences.

The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP (2015)
Journal Article
Lewis, J. E., Brameld, J. M., Hill, P., Barrett, P., Ebling, F. J., & Jethwa, P. (in press). The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP. Journal of Neuroscience Methods, 256, https://doi.org/10.1016/j.jneumeth.2015.08.013

Introduction: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over-expression of two genes and in combination with recombinant adeno-associated viruses (rAAV) has bee... Read More about The use of viral 2A sequence for the simultaneous over-expression of both the vgf gene and enhanced green fluorescent protein eGFP.

Dual effects of fibroblast growth factor 21 on hepatic energy metabolism (2015)
Journal Article
Samms, R. J., Murphy, M., Fowler, M. J., Cooper, S., Emmerson, P., Coskun, T., …Tsintzas, K. (2015). Dual effects of fibroblast growth factor 21 on hepatic energy metabolism. Journal of Endocrinology, 227(1), https://doi.org/10.1530/JOE-15-0334

The aim of this study was to investigate the mechanisms by which FGF21 affects hepatic integration of carbohydrate and fat metabolism in Siberian hamsters, a natural model of adiposity. Twelve aged matched adult male Siberian hamsters maintained in t... Read More about Dual effects of fibroblast growth factor 21 on hepatic energy metabolism.

Endosymbiotic origin and differential loss of eukaryotic genes (2015)
Journal Article
Ku, C., Nelson-Sathi, S., Roettger, M., Sousa, F. L., Lockhart, P. J., Bryant, D., …Martin, W. F. (2015). Endosymbiotic origin and differential loss of eukaryotic genes. Nature, 524(7566), 427–432. https://doi.org/10.1038/nature14963

Chloroplasts arose from cyanobacteria, mitochondria arose from proteobacteria. Both organelles have conserved their prokaryotic biochemistry, but their genomes are reduced, and most organelle proteins are encoded in the nucleus. Endosymbiotic theory... Read More about Endosymbiotic origin and differential loss of eukaryotic genes.

Primordial germ cells: the first cell lineage or the last cells standing? (2015)
Journal Article
Johnson, A. D., & Alberio, R. (in press). Primordial germ cells: the first cell lineage or the last cells standing?. Development, 142, https://doi.org/10.1242/dev.113993

Embryos of many animal models express germ line determinants that suppress transcription and mediate early germ line commitment, which occurs before the somatic cell lineages are established. However, not all animals segregate their germ line in this... Read More about Primordial germ cells: the first cell lineage or the last cells standing?.