All Outputs (3)

Screening Mutants by Single Fly Genomic PCR (2022)
Book Chapter
Nagarajan, U., & Georgiou, M. (2022). Screening Mutants by Single Fly Genomic PCR. In Notch Signaling Research (15-25). Humana Press. https://doi.org/10.1007/978-1-0716-2201-8_2

Imprecise P-element excision or FRT-mediated recombination is routinely performed to mutagenize a gene of interest. It is, however, tedious to maintain all independent and individual excised mutant fly lines before the presence of a mutation is confi... Read More about Screening Mutants by Single Fly Genomic PCR.

A Workflow to Track and Analyze Endothelial Migration During Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy (2022)
Book Chapter
Wilkinson, R. N. (2022). A Workflow to Track and Analyze Endothelial Migration During Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy. . Springer US. https://doi.org/10.1007/978-1-0716-2059-5_2

Zebrafish allow unrivalled in vivo imaging of vascular development due to their optical translucency and the availability of transgenic lines which fluorescently label cells and tissues of interest. Advances in light sheet fluorescence microscopy all... Read More about A Workflow to Track and Analyze Endothelial Migration During Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy.

In Vivo cAMP Dynamics in Drosophila Larval Neurons (2022)
Book Chapter
Maiellaro, I. (2022). In Vivo cAMP Dynamics in Drosophila Larval Neurons. In M. Zaccolo (Ed.), cAMP Signaling: Methods and Protocols (181-194). New York: Humana Press. https://doi.org/10.1007/978-1-0716-2245-2_11

Cyclic adenosine monophosphate (cAMP) is a universal second messenger that mediates a myriad of cell functions across all kingdoms of life. The ability to monitor intracellular changes of cAMP concentration in living cells using FRET-based biosensors... Read More about In Vivo cAMP Dynamics in Drosophila Larval Neurons.