Screening Mutants by Single Fly Genomic PCR

Nagarajan, Usha; Georgiou, Marios

Abstract

Imprecise P-element excision or FRT-mediated recombination is routinely performed to mutagenize a gene of interest. It is, however, tedious to maintain all independent and individual excised mutant fly lines before the presence of a mutation is confirmed. Here, we provide a method to detect and confirm the presence of a mutation, as and when mutant flies are generated. By allowing for the maintenance and expansion of only the confirmed mutant lines, this protocol will help to save time, money, and space.

Citation

Nagarajan, U., & Georgiou, M. (2022). Screening Mutants by Single Fly Genomic PCR. In Notch Signaling Research (15-25). Humana Press. https://doi.org/10.1007/978-1-0716-2201-8_2

• Online Publication Date: Jun 9, 2022
• Publication Date: 2022
• Deposit Date: Jun 17, 2022
• Publicly Available Date: Jun 10, 2023
• Publisher: Humana Press
• Pages: 15-25
• Series Title: Methods in Molecular Biology
• Series Number: 2472
• Series ISSN: 1064-3745
• Book Title: Notch Signaling Research
• ISBN: 9781071622001
• DOI: https://doi.org/10.1007/978-1-0716-2201-8_2
• Public URL: https://nottingham-repository.worktribe.com/output/8500703
• Publisher URL: https://link.springer.com/protocol/10.1007/978-1-0716-2201-8_2