Skip to main content

Research Repository

Advanced Search

All Outputs (626)

Use of the Affymetrix human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes (2011)
Journal Article
Graham, N. S., May, S. T., Daniel, Z. C., Emmerson, Z. F., Brameld, J. M., & Parr, T. (2011). Use of the Affymetrix human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes. Animal, 5(6), 861-866. https://doi.org/10.1017/S1751731110002533

Affymetrix GeneChip microarrays are a powerful tool to study global gene expression profiles and have been used on many species. However, no sheep-specific Affymetrix GeneChip is currently available and the bovine array is fairly limited in its cover... Read More about Use of the Affymetrix human GeneChip array and genomic DNA hybridisation probe selection to study ovine transcriptomes.

Stochastic and deterministic multiscale models for systems biology: An auxin-transport case study (2010)
Journal Article
Twycross, J., Band, L. R., Bennett, M. J., King, J. R., & Krasnogor, N. (2010). Stochastic and deterministic multiscale models for systems biology: An auxin-transport case study. BMC Systems Biology, 4, https://doi.org/10.1186/1752-0509-4-34

Background: Stochastic and asymptotic methods are powerful tools in developing multiscale systems biology models; however, little has been done in this context to compare the efficacy of these methods. The majority of current systems biology modellin... Read More about Stochastic and deterministic multiscale models for systems biology: An auxin-transport case study.

Differential Yeast Gene Transcription During Brewery Propagation (2010)
Journal Article
Gibson, B. R., Graham, N. S., Boulton, C. A., Box, W. G., Lawrence, S. J., Linforth, R. S. T., May, S. T., & Smart, K. A. (2010). Differential Yeast Gene Transcription During Brewery Propagation. Journal of The American Society of Brewing Chemists, 68(1), 21-29. https://doi.org/10.1094/ASBCJ-2009-1123-01

The aim of this study was to determine the transcriptomic response of Saccharomyces pastorianus to environmental change during brewery propagation. Utilization of amino acids and fermentable carbohydrates during fullscale brewery propagation was comp... Read More about Differential Yeast Gene Transcription During Brewery Propagation.

Evaluation of pathogenicity and aggressiveness of F. langsethiae on oat and wheat seedlings relative to known seedling blight pathogens (2009)
Journal Article
Imathiu, S. M., Hare, M. C., Ray, R. V., Back, M., & Edwards, S. G. (2010). Evaluation of pathogenicity and aggressiveness of F. langsethiae on oat and wheat seedlings relative to known seedling blight pathogens. European Journal of Plant Pathology, 126(2), 203-216. https://doi.org/10.1007/s10658-009-9533-0

Fusarium and Microdochium species are causal agents of seedling blight of small-grain cereal crops where they may contribute to a significant reduction in crop establishment and final yield. Two experiments were carried out to investigate the potenti... Read More about Evaluation of pathogenicity and aggressiveness of F. langsethiae on oat and wheat seedlings relative to known seedling blight pathogens.

Amino Acid Uptake and Yeast Gene Transcription During Industrial Brewery Fermentation (2009)
Journal Article
Gibson, B. R., Boulton, C. A., Box, W. G., Graham, N. S., Lawrence, S. J., Linforth, R. S., & Smart, K. A. (2009). Amino Acid Uptake and Yeast Gene Transcription During Industrial Brewery Fermentation. Journal- American Society of Brewing Chemists, 67(3), 157-165. https://doi.org/10.1094/ASBCJ-2009-0720-01

Amino acid utilization by yeast during brewer's wort fermentation influences both the fermentation performance and flavor profile of the finished product. To better understand the relationship between the yeast cell and wort amino acid composition, o... Read More about Amino Acid Uptake and Yeast Gene Transcription During Industrial Brewery Fermentation.

Modular assembly of cell systems biology models using P systems (2009)
Presentation / Conference Contribution
Romero-Campero, F. J., Twycross, J., Camara, M., Bennett, M., Gheorghe, M., & Krasnogor, N. (2008, June). Modular assembly of cell systems biology models using P systems. Presented at Prague International Workshop on Membrane Computing, Prague, Czech Republic

In this paper we propose an extension of a systems/synthetic biology modelling framework based on P systems that explicitly includes modularity. Modularisation in cellular systems can be produced by chemical specificity, spatial localisation and/or t... Read More about Modular assembly of cell systems biology models using P systems.

Fusarium langsethiae pathogenicity and aggressiveness towards oats and wheat in wounded and unwounded in vitro detached leaf assays (2008)
Journal Article
Imathiu, S. M., Ray, R. V., Back, M., Hare, M. C., & Edwards, S. G. (2009). Fusarium langsethiae pathogenicity and aggressiveness towards oats and wheat in wounded and unwounded in vitro detached leaf assays. European Journal of Plant Pathology, 124(1), 117-126. https://doi.org/10.1007/s10658-008-9398-7

In vitro detached leaf assays involving artificial inoculation of wounded and unwounded oat and wheat leaves were used to investigate the potential pathogenicity and aggressiveness of F. langsethiae, which was linked recently to the production of typ... Read More about Fusarium langsethiae pathogenicity and aggressiveness towards oats and wheat in wounded and unwounded in vitro detached leaf assays.

The control of chlorophyll catabolism and the status of yellowing as a biomarker of leaf senescence (2008)
Journal Article
Ougham, H., Hörtensteiner, S., Armstead, I., Donnison, I., King, I., Thomas, H., & Mur, L. (2008). The control of chlorophyll catabolism and the status of yellowing as a biomarker of leaf senescence. Plant Biology, 10, 4-14. https://doi.org/10.1111/j.1438-8677.2008.00081.x

The pathway of chlorophyll catabolism during leaf senescence is known in a fair amount of biochemical and cell biological detail. In the last few years, genes encoding a number of the catabolic enzymes have been characterized, including the key ring‐... Read More about The control of chlorophyll catabolism and the status of yellowing as a biomarker of leaf senescence.

Effect of timing of fungicide application on the development of fusarium head blight and the accumulation of deoxynivalenol (DON) in winter wheat grain (2008)
Journal Article
Pirgozliev, S. R., Ray, R. V., Edwards, S. G., Hare, M. C., & Jenkinson, P. (2008). Effect of timing of fungicide application on the development of fusarium head blight and the accumulation of deoxynivalenol (DON) in winter wheat grain. Cereal Research Communications, 36(2), 289-299. https://doi.org/10.1556/CRC.36.2008.2.9

The effect of different timings of fungicide applications on Fusarium head blight severity and mycotoxin accumulation in wheat grain was investigated in two field experiments. The fungicides metconazole, tebuconazole, azoxystrobin and mixtures of met... Read More about Effect of timing of fungicide application on the development of fusarium head blight and the accumulation of deoxynivalenol (DON) in winter wheat grain.

Development of a Genomic Microsatellite Library in Perennial Ryegrass (Lolium perenne) and its Use in Trait Mapping (2008)
Journal Article
King, J., Thorogood, D., Edwards, K. J., Armstead, I. P., Roberts, L., Skot, K., …King, I. P. (in press). Development of a Genomic Microsatellite Library in Perennial Ryegrass (Lolium perenne) and its Use in Trait Mapping. Annals of Botany, 101(6), 845-853. https://doi.org/10.1093/aob/mcn016

Background and Aims
Perennial ryegrass (Lolium perenne) is one of the key forage and amenity grasses throughout the world. In the UK it accounts for 70 % of all agricultural land use with an estimated farm gate value of £6 billion per annum. However... Read More about Development of a Genomic Microsatellite Library in Perennial Ryegrass (Lolium perenne) and its Use in Trait Mapping.

Identifying genetic components controlling fertility in the outcrossing grass species perennial ryegrass (Lolium perenne) by quantitative trait loci analysis and comparative genetics (2008)
Journal Article
Armstead, I. P., Turner, L. B., Marshall, A. H., Humphreys, M. O., King, I. P., & Thorogood, D. (2008). Identifying genetic components controlling fertility in the outcrossing grass species perennial ryegrass (Lolium perenne) by quantitative trait loci analysis and comparative genetics. New Phytologist, 178(3), 559-571. https://doi.org/10.1111/j.1469-8137.2008.02413.x

Mutational load and resource allocation factors and their effects on limiting seed set were investigated in ryegrass by comparative mapping genomics and quantitative trait loci (QTL) analysis in two perennial ryegrass (Lolium perenne) mapping familie... Read More about Identifying genetic components controlling fertility in the outcrossing grass species perennial ryegrass (Lolium perenne) by quantitative trait loci analysis and comparative genetics.

Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation (2007)
Journal Article
Armstead, I., Huang, L., King, J., Ougham, H., Thomas, H., & King, I. (2007). Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation. BMC Genomics, 8(1), Article 283. https://doi.org/10.1186/1471-2164-8-283

Background
Various methods have been developed to explore inter-genomic relationships among plant species. Here, we present a sequence similarity analysis based upon comparison of transcript-assembly and methylation-filtered databases from five plan... Read More about Rice pseudomolecule-anchored cross-species DNA sequence alignments indicate regional genomic variation in expressed sequence conservation.

From phenotype to genotype: whole tissue profiling for plant breeding (2007)
Journal Article
Goodacre, R., Roberts, L., Ellis, D. I., Thorogood, D., Reader, S. M., Ougham, H., & King, I. (2007). From phenotype to genotype: whole tissue profiling for plant breeding. Metabolomics, 3(4), 489-501. doi:10.1007/s11306-007-0062-6

Fourier transform infrared spectroscopy (FT-IR) was used to obtain ‘holistic’ metabolic fingerprints from a wide range of plants to differentiate species, population, single plant genotype, and chromosomal constitution differences. Sample preparation... Read More about From phenotype to genotype: whole tissue profiling for plant breeding.

Introgression mapping in the grasses (2007)
Journal Article
King, J., Armstead, I. P., Donnison, I. S., Harper, J. A., Roberts, L. A., Thomas, H., …King, I. P. (2007). Introgression mapping in the grasses. Chromosome Research, 15(1), 105-113. https://doi.org/10.1007/s10577-006-1103-0

The unique properties of Lolium/Festuca hybrids and their derivatives provide an ideal system for intergeneric introgression. At IGER a focus on the Lolium perenne/Festuca pratensis system is being exploited to elucidate genome organization in the gr... Read More about Introgression mapping in the grasses.

Cross-Species Identification of Mendel's I Locus (2007)
Journal Article
Armstead, I., Donnison, I., Aubry, S., Harper, J., Hortensteiner, S., James, C., …King, I. (2007). Cross-Species Identification of Mendel's I Locus. Science, 315(5808), 73-73. https://doi.org/10.1126/science.1132912

A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using class... Read More about Cross-Species Identification of Mendel's I Locus.

Quantitative Fusarium spp. and Microdochium spp. PCR assays to evaluate seed treatments for the control of Fusarium seedling blight of wheat (2006)
Journal Article
Glynn, N. C., Ray, R., Edwards, S. G., Hare, M. C., Parry, D. W., Barnett, C. J., & Beck, J. J. (2007). Quantitative Fusarium spp. and Microdochium spp. PCR assays to evaluate seed treatments for the control of Fusarium seedling blight of wheat. Journal of Applied Microbiology, 102(6), 1645-1653. https://doi.org/10.1111/j.1365-2672.2006.03190.x

Aims: To develop sensitive quantitative PCR assays for the two groups of pathogens responsible for Fusarium seedling blight in wheat: Fusarium group (Fusarium culmorum and Fusarium graminearum) and Microdochium group (Microdochium nivale and Microdoc... Read More about Quantitative Fusarium spp. and Microdochium spp. PCR assays to evaluate seed treatments for the control of Fusarium seedling blight of wheat.

Developing digital records: Early experiences of record and replay (2006)
Journal Article
Crabtree, A., French, A., Greenhalgh, C., Benford, S., Cheverst, K., Fitton, D., …Graham, C. (2006). Developing digital records: Early experiences of record and replay. Computer Supported Cooperative Work, 15(4), 281-319. https://doi.org/10.1007/s10606-006-9026-z

In this paper we consider the development of 'digital records' to support ethnographic study of interaction and collaboration in ubiquitous computing environments and articulate the core concept of 'record and replay' through two case studies. One fo... Read More about Developing digital records: Early experiences of record and replay.

Effect of eyespot caused by Oculimacula yallundae and O. acuformis, assessed visually and by competitive PCR, on stem strength associated with lodging resistance and yield of winter wheat (2006)
Journal Article
Ray, R. V., Crook, M., Jenkinson, P., & Edwards, S. (2006). Effect of eyespot caused by Oculimacula yallundae and O. acuformis, assessed visually and by competitive PCR, on stem strength associated with lodging resistance and yield of winter wheat. Journal of Experimental Botany, 57(10), 2249-2257. https://doi.org/10.1093/jxb/erj192

Winter wheat, (cv. Consort) was inoculated with three isolates of either Oculimacula yallundae or O. acuformis to determine the effect of eyespot caused by each species on yield and lodging resistance of winter wheat. Plants were visually assessed fo... Read More about Effect of eyespot caused by Oculimacula yallundae and O. acuformis, assessed visually and by competitive PCR, on stem strength associated with lodging resistance and yield of winter wheat.

Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization (2006)
Journal Article
Hasterok, R., Marasek, A., Donnison, I. S., Armstead, I., Thomas, A., King, I. P., …Jenkins, G. (2006). Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization. Genetics, 173(1), 349-362. https://doi.org/10.1534/genetics.105.049726

As part of an initiative to develop Brachypodium distachyon as a genomic “bridge” species between rice and the temperate cereals and grasses, a BAC library has been constructed for the two diploid (2n = 2x = 10) genotypes, ABR1 and ABR5. The library... Read More about Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization.

Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis (2004)
Journal Article
Moore, B. J., Donnison, I. S., Harper, J. A., Armstead, I. P., King, J., Thomas, H., …King, I. P. (2005). Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis. New Phytologist, 165(3), 801-806. https://doi.org/10.1111/j.1469-8137.2004.01269.x

Intergeneric hybrids between Lolium multiflorum and Festuca pratensis (Lm/Fp) and their derivatives exhibit a unique combination of genetic and cytogenetic characteristics: chromosomes undergo a high frequency of homoeologous recombination at meiosis... Read More about Molecular tagging of a senescence gene by introgression mapping of a stay-green mutation from Festuca pratensis.