Cross-Species Identification of Mendel's I Locus

Armstead, Ian; Donnison, Iain; Aubry, Sylvain; Harper, John; Hortensteiner, Stefan; James, Caron; Mani, Jan; Moffet, Matt; Ougham, Helen; Roberts, Luned; Thomas, Ann; Weeden, Norman; Thomas, Howard; King, Ian

Abstract

A key gene involved in plant senescence, mutations of which partially disable chlorophyll catabolism and confer stay-green leaf and cotyledon phenotypes, has been identified in Pisum sativum, Arabidopsis thaliana, and Festuca pratensis by using classical and molecular genetics and comparative genomics. A stay-green locus in F. pratensis is syntenically equivalent to a similar stay-green locus on rice chromosome 9. Functional testing in Arabidopsis of a homolog of the rice candidate gene revealed (i) senescence-associated gene expression and (ii) a stay-green phenotype after RNA interference silencing. Genetic mapping in pea demonstrated cosegregation with the yellow/green cotyledon polymorphism (I/i) first reported by Gregor Mendel in 1866.

Citation

Armstead, I., Donnison, I., Aubry, S., Harper, J., Hortensteiner, S., James, C., …King, I. (2007). Cross-Species Identification of Mendel's I Locus. Science, 315(5808), 73-73. https://doi.org/10.1126/science.1132912

• Journal Article Type: Article
• Acceptance Date: Oct 16, 2006
• Publication Date: Jan 5, 2007
• Deposit Date: Dec 6, 2018
• Journal: Science
• Print ISSN: 0036-8075
• Electronic ISSN: 1095-9203
• Publisher: American Association for the Advancement of Science
• Peer Reviewed: Peer Reviewed
• Volume: 315
• Issue: 5808
• Pages: 73-73
• DOI: https://doi.org/10.1126/science.1132912
• Public URL: https://nottingham-repository.worktribe.com/output/1378667
• Publisher URL: http://science.sciencemag.org/content/315/5808/73