Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization

Hasterok, Robert; Marasek, Agnieszka; Donnison, Iain S.; Armstead, Ian; Thomas, Ann; King, Ian P.; Wolny, Elzbieta; Idziak, Dominika; Draper, John; Jenkins, Glyn

Abstract

As part of an initiative to develop Brachypodium distachyon as a genomic “bridge” species between rice and the temperate cereals and grasses, a BAC library has been constructed for the two diploid (2n = 2x = 10) genotypes, ABR1 and ABR5. The library consists of 9100 clones, with an approximate average insert size of 88 kb, representing 2.22 genome equivalents. To validate the usefulness of this species for comparative genomics and gene discovery in its larger genome relatives, the library was screened by PCR using primers designed on previously mapped rice and Poaceae sequences. Screening indicated a degree of synteny between these species and B. distachyon, which was confirmed by fluorescent in situ hybridization of the marker-selected BACs (BAC landing) to the 10 chromosome arms of the karyotype, with most of the BACs hybridizing as single loci on known chromosomes. Contiguous BACs colocalized on individual chromosomes, thereby confirming the conservation of genome synteny and proving that B. distachyon has utility as a temperate grass model species alternative to rice.

Citation

Hasterok, R., Marasek, A., Donnison, I. S., Armstead, I., Thomas, A., King, I. P., …Jenkins, G. (2006). Alignment of the Genomes of Brachypodium distachyon and Temperate Cereals and Grasses Using Bacterial Artificial Chromosome Landing With Fluorescence in Situ Hybridization. Genetics, 173(1), 349-362. https://doi.org/10.1534/genetics.105.049726

• Journal Article Type: Article
• Acceptance Date: Feb 14, 2006
• Online Publication Date: Mar 17, 2006
• Publication Date: May 1, 2006
• Deposit Date: Dec 6, 2018
• Journal: Genetics
• Print ISSN: 0016-6731
• Publisher: Genetics Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 173
• Issue: 1
• Pages: 349-362
• DOI: https://doi.org/10.1534/genetics.105.049726
• Public URL: https://nottingham-repository.worktribe.com/output/1378788
• Publisher URL: https://www.genetics.org/content/173/1/349