All Outputs (10)

Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases (2024)
Journal Article
Zheng, X., Chacón, M., Allers, T., Dixon, N., & Winterburn, J. (2024). Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases. Biochemical Engineering Journal, 212, Article 109498. https://doi.org/10.1016/j.bej.2024.109498

The polyhydroxyalkanoate terpolymer, P[(3HB)-co-(3HV)-co-(4HB)], is a promising plastic alternative for specialized applications, notably in medical and pharmaceutical sectors. Haloferax mediterranei (Hfx), an extreme halophile arch... Read More about Enhanced 4-hydroxybutyrate incorporation into the PHA terpolymer of Haloferax mediterranei by heterologous expression of 4-hydroxybutyrate-CoA transferases/synthetases.

A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification (2024)
Journal Article
Karan, R., Renn, D., Allers, T., & Rueping, M. (2024). A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification. Frontiers in Microbiology, 15, Article 1403623. https://doi.org/10.3389/fmicb.2024.1403623

Extremophilic proteins are valuable in various fields, but their expression can be challenging in traditional hosts like Escherichia coli due to misfolding and aggregation. Haloferax volcanii (H. volcanii), a halophilic expression system, offers a so... Read More about A systematic analysis of affinity tags in the haloarchaeal expression system, Haloferax volcanii for protein purification.

Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing (2023)
Journal Article
Lever, R., Simmons, E., Gamble-Milner, R., Buckley, R., Harrison, C., Parkes, A., …Allers, T. (2023). Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing. Nucleic Acids Research, 51(16), 8563-8574. https://doi.org/10.1093/nar/gkad572

Hel308 helicases promote genome stability in archaea and are conserved in metazoans, where they are known as HELQ. Their helicase mechanism is well characterised, but it is unclear how they specifically contribute to genome stability in archaea. We s... Read More about Archaeal Hel308 suppresses recombination through a catalytic switch that controls DNA annealing.

DNA repair in the archaea: an emerging picture (2018)
Journal Article
White, M. F., & Allers, T. (2018). DNA repair in the archaea: an emerging picture. FEMS Microbiology Reviews, 42(4), 514-526. https://doi.org/10.1093/femsre/fuy020

There has long been a fascination in the DNA Repair pathways of archaea, for two main reasons. Firstly, many archaea inhabit extreme environments where the rate of physical damage to DNA is accelerated. These archaea might reasonably be expected to h... Read More about DNA repair in the archaea: an emerging picture.

Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii (2018)
Journal Article
Ausiannikava, D., Mitchell, L., Marriott, H., Smith, V., Hawkins, M., Makarova, K. S., Koonin, E. V., Nieduszynski, C. A., & Allers, T. (2018). Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii. Molecular Biology and Evolution, 35(8), 1855-1868. https://doi.org/10.1093/molbev/msy075

The common ancestry of archaea and eukaryotes is evident in their genome architecture. All eukaryotic and several archaeal genomes consist of multiple chromosomes, each replicated from multiple origins. Three scenarios have been proposed for the evol... Read More about Evolution of genome architecture in archaea: spontaneous generation of a new chromosome in Haloferax volcanii.

RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator (2017)
Journal Article
Wardell, K., Haldenby, S., Jones, N., Liddell, S., Ngo, G. H., & Allers, T. (2017). RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator. DNA Repair, 55, https://doi.org/10.1016/j.dnarep.2017.04.005

Homologous recombination plays a central role in the repair of double-strand DNA breaks, the restart of stalled replication forks and the generation of genetic diversity. Regulation of recombination is essential since defects can lead to genome insta... Read More about RadB acts in homologous recombination in the archaeon Haloferax volcanii, consistent with a role as recombination mediator.

High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon (2017)
Journal Article
Stachler, A.-E., Turgeman-Grott, I., Shtifman-Segal, E., Allers, T., Marchfelder, A., & Gophna, U. (2017). High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon. Nucleic Acids Research, 45(9), 5208-5216. https://doi.org/10.1093/nar/gkx150

CRISPR-Cas systems allow bacteria and archaea to acquire sequence-specific immunity against selfish genetic elements such as viruses and plasmids, by specific degradation of invader DNA or RNA. However, this involves the risk of autoimmunity if immun... Read More about High tolerance to self-targeting of the genome by the endogenous CRISPR-Cas system in an archaeon.

Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor (2015)
Journal Article
Strillinger, E., Grötzinger, S. W., Allers, T., Eppinger, J., & Weuster-Botz, D. (2016). Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor. Applied Microbiology and Biotechnology, 100(3), 1183-1195. https://doi.org/10.1007/s00253-015-7007-1

The success of biotechnological processes is based on the availability of efficient and highly specific biocatalysts, which can satisfy industrial demands. Extreme and remote environments like the deep brine pools of the Red Sea represent highly inte... Read More about Production of halophilic proteins using Haloferax volcanii H1895 in a stirred-tank bioreactor.

DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii (2013)
Journal Article
Delmas, S., Duggin, I. J., & Allers, T. (2013). DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii. Molecular Microbiology, 87(1), https://doi.org/10.1111/mmi.12091

In prokaryotes the genome is organized in a dynamic
structure called the nucleoid, which is embedded in
the cytoplasm. We show here that in the archaeon
Haloferax volcanii, compaction and reorganization of
the nucleoid is induced by stresses that... Read More about DNA damage induces nucleoid compaction via the Mre11-Rad50 complex in the archaeon Haloferax volcanii.

Archaeal genetics – the third way (2005)
Journal Article
Allers, T., & Mevarech, M. (2005). Archaeal genetics – the third way. Nature Genetics, 6, https://doi.org/10.1038/nrg1504

For decades, archaea were misclassified as bacteria on account of their prokaryotic morphology. Molecular phylogeny eventually revealed that archaea, like bacteria and eukaryotes, are a fundamentally distinct domain of life. Genome analyses have conf... Read More about Archaeal genetics – the third way.