Skip to main content

Research Repository

Advanced Search

All Outputs (108)

Bursting the bubble on bacterial biofilms: a flow cell methodology (2012)
Journal Article
Crusz, S. A., Popat, R., Rybtke, M. T., Cámara, M., Givskov, M., Tolker-Nielsen, T., Diggle, S. P., & Williams, P. (2012). Bursting the bubble on bacterial biofilms: a flow cell methodology. Biofouling, 28(8), https://doi.org/10.1080/08927014.2012.716044

The flow cell biofilm system is an important and widely used tool for the in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. This paper provides an introduction to the background and use of such systems,... Read More about Bursting the bubble on bacterial biofilms: a flow cell methodology.

A glycopeptide dendrimer inhibitor of the galactose specific lectin LecA & of Pseudomonas aeruginosa biofilms (2011)
Journal Article
Kadam, R. U., Bergmann, M., Hurley, M., Garg, D., Cacciarini, M., Swiderska, M. A., Nativi, C., Sattler, M., Smyth, A. R., Williams, P., Cámara, M., Stocker, A., Darbre, T., & Reymond, J.-L. (2011). A glycopeptide dendrimer inhibitor of the galactose specific lectin LecA & of Pseudomonas aeruginosa biofilms. Angewandte Chemie International Edition, 50(45), https://doi.org/10.1002/anie.201104342

Biofilm inhibition is achieved with a phenylgalactosyl peptide dendrimer (see picture) that binds to the galactose-specific lectin LecA of P. aeruginosa. The multivalency of the ligands is critical for biofilm inhibition, although the nature of the l... Read More about A glycopeptide dendrimer inhibitor of the galactose specific lectin LecA & of Pseudomonas aeruginosa biofilms.

Garlic as an inhibitor of pseudomonas aeruginosa quorum sensing in cystic fibrosis - A pilot randomized controlled trial (2010)
Journal Article
Smyth, A. R., Cifelli, P. M., Ortori, C. A., Righetti, K., Lewis, S., Erskine, P., Holland, E. D., Givskov, M., Williams, P., Cámara, M., Barrett, D. A., & Knox, A. (2010). Garlic as an inhibitor of pseudomonas aeruginosa quorum sensing in cystic fibrosis - A pilot randomized controlled trial. Pediatric Pulmonology, 45(4), 356-362. https://doi.org/10.1002/ppul.21193

Pseudomonas aeruginosa forms biofilms in the cystic fibrosis lung. Quorum sensing (QS) controls biofilm maturation, immune evasion, antibiotic tolerance and virulence factor production. Garlic shows QS inhibitory activity in vitro and in animal model... Read More about Garlic as an inhibitor of pseudomonas aeruginosa quorum sensing in cystic fibrosis - A pilot randomized controlled trial.

High throughput methods applied in biomaterial development and discovery (2010)
Journal Article
Hook, A. L., Anderson, D. G., Langer, R., Williams, P., Davies, M. C., & Alexander, M. R. (2010). High throughput methods applied in biomaterial development and discovery. Biomaterials, 31(2), https://doi.org/10.1016/j.biomaterials.2009.09.037

The high throughput discovery of new materials can be achieved by rapidly screening many different materials synthesised by a combinatorial approach to identify the optimal material that fulfils a particular biomedical application. Here we review the... Read More about High throughput methods applied in biomaterial development and discovery.

Differential Recognition of Staphylococcus aureus Quorum-Sensing Signals Depends on Both Extracellular Loops 1 and 2 of the Transmembrane Sensor AgrC (2008)
Journal Article
Jensen, R. O., Winzer, K., Clarke, S. R., Chan, W. C., & Williams, P. (2008). Differential Recognition of Staphylococcus aureus Quorum-Sensing Signals Depends on Both Extracellular Loops 1 and 2 of the Transmembrane Sensor AgrC. Journal of Molecular Biology, 381(2), 300-309. https://doi.org/10.1016/j.jmb.2008.06.018

Virulence in Staphylococcus aureus is regulated via agr-dependent quorum sensing in which an autoinducing peptide (AIP) activates AgrC, a histidine protein kinase. AIPs are usually thiolactones containing seven to nine amino acid residues in which th... Read More about Differential Recognition of Staphylococcus aureus Quorum-Sensing Signals Depends on Both Extracellular Loops 1 and 2 of the Transmembrane Sensor AgrC.

Look who's talking: Communication and quorum sensing in the bacterial world (2007)
Journal Article
Williams, P., Winzer, K., Chan, W. C., & Cámara, M. (2007). Look who's talking: Communication and quorum sensing in the bacterial world. Philosophical Transactions B: Biological Sciences, 362(1483), 1119-1134. https://doi.org/10.1098/rstb.2007.2039

For many years bacteria were considered primarily as autonomous unicellular organisms with little capacity for collective behaviour. However, we now appreciate that bacterial cells are in fact, highly communicative. The generic term 'quorum sensing'... Read More about Look who's talking: Communication and quorum sensing in the bacterial world.

Side-chain-to-tail thiolactone peptide inhibitors of the staphylococcal quorum-sensing system (2003)
Journal Article
Scott, R. J., Lian, L. Y., Muharram, S. H., Cockayne, A., Wood, S. J., Bycroft, B. W., Williams, P., & Chan, W. C. (2003). Side-chain-to-tail thiolactone peptide inhibitors of the staphylococcal quorum-sensing system. Bioorganic and Medicinal Chemistry Letters, 13(15), 2449-2453. https://doi.org/10.1016/S0960-894X%2803%2900497-9

The expression of many staphylococcal virulence factors are regulated by the agr locus via a two-component signal transduction system (TCSTS), which is activated in response to a secreted autoinducer peptide (AIP). By exploiting the unique chemical a... Read More about Side-chain-to-tail thiolactone peptide inhibitors of the staphylococcal quorum-sensing system.