All Outputs (19)

Alkyl-quinolone-dependent quorum sensing controls prophage-mediated autolysis in Pseudomonas aeruginosa colony biofilms (2023)
Journal Article
Giallonardi, G., Letizia, M., Mellini, M., Frangipani, E., Halliday, N., Heeb, S., …Rampioni, G. (2023). Alkyl-quinolone-dependent quorum sensing controls prophage-mediated autolysis in Pseudomonas aeruginosa colony biofilms. Frontiers in Cellular and Infection Microbiology, 13, Article 1183681. https://doi.org/10.3389/fcimb.2023.1183681

Pseudomonas aeruginosa is a model quorum sensing (QS) pathogen with three interconnected QS circuits that control the production of virulence factors and antibiotic tolerant biofilms. The pqs QS system of P. aeruginosa is responsible for the biosynth... Read More about Alkyl-quinolone-dependent quorum sensing controls prophage-mediated autolysis in Pseudomonas aeruginosa colony biofilms.

Growth rate and nutrient limitation as key drivers of extracellular quorum sensing signal molecule accumulation in Pseudomonas aeruginosa (2023)
Journal Article
Dubern, J.-F., Halliday, N., Cámara, M., Winzer, K., Barrett, D. A., Hardie, K. R., & Williams, P. (2023). Growth rate and nutrient limitation as key drivers of extracellular quorum sensing signal molecule accumulation in Pseudomonas aeruginosa. Microbiology, 169(4), Article 001316. https://doi.org/10.1099/mic.0.001316

In Pseudomonas aeruginosa, quorum sensing (QS) depends on an interconnected regulatory hierarchy involving the Las, Rhl and Pqs systems, which are collectively responsible for the co-ordinated synthesis of a diverse repertoire of N-acylhomoserine lac... Read More about Growth rate and nutrient limitation as key drivers of extracellular quorum sensing signal molecule accumulation in Pseudomonas aeruginosa.

Symbiopectobacterium purcellii, gen. nov., sp. nov., isolated from the leafhopper Empoasca decipiens (2022)
Journal Article
Nadal-Jimenez, P., Siozios, S., Halliday, N., Cámara, M., & Hurst, G. D. D. (2022). Symbiopectobacterium purcellii, gen. nov., sp. nov., isolated from the leafhopper Empoasca decipiens. International Journal of Systematic and Evolutionary Microbiology, 72(6), Article 005440. https://doi.org/10.1099/ijsem.0.005440

Bacterial endosymbionts are found in multiple arthropod species, where they play crucial roles as nutritional symbionts, defensive symbionts or reproductive parasites. Recent work has highlighted a new clade of heritable microbes within the gammaprot... Read More about Symbiopectobacterium purcellii, gen. nov., sp. nov., isolated from the leafhopper Empoasca decipiens.

2-Alkyl-4-quinolone quorum sensing molecules are biomarkers for culture-independent Pseudomonas aeruginosa burden in adults with cystic fibrosis (2021)
Journal Article
Zain, N. M. M., Webb, K., Stewart, I., Halliday, N., Barrett, D. A., Nash, E. F., …Barr, H. L. (2021). 2-Alkyl-4-quinolone quorum sensing molecules are biomarkers for culture-independent Pseudomonas aeruginosa burden in adults with cystic fibrosis. Journal of Medical Microbiology, 70(10), https://doi.org/10.1099/jmm.0.001420

Introduction. Pseudomonas aeruginosa produces quorum sensing signalling molecules including 2-alkyl-4-quinolones (AQs), which regulate virulence factor production in the cystic fibrosis (CF) airways. Hypothesis/Gap statement. Culture can lead to cond... Read More about 2-Alkyl-4-quinolone quorum sensing molecules are biomarkers for culture-independent Pseudomonas aeruginosa burden in adults with cystic fibrosis.

Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis (2021)
Journal Article
Webb, K., Cámara, M., Zain, N. M. M., Halliday, N., Bruce, K. D., Nash, E. F., …Barr, H. L. (2022). Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis. Journal of Cystic Fibrosis, 21(4), 626-629. https://doi.org/10.1016/j.jcf.2021.08.030

Pseudomonas aeruginosa produces specific signalling molecules, 2-alkyl-4-quinolones (AQs) that are detectable in the sputum of adults with cystic fibrosis (CF) and who have pulmonary infection with this opportunistic pathogen. This study aimed to det... Read More about Novel detection of specific bacterial quorum sensing molecules in saliva: Potential non-invasive biomarkers for pulmonary Pseudomonas aeruginosa in cystic fibrosis.

Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1sub-unit (2021)
Journal Article
Soh, E. Y.-C., Smith, F., Gimenez, M. R., Yang, L., Vejborg, R. M., Fletcher, M., …Williams, P. (2021). Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1sub-unit. PLoS Pathogens, 17(8), Article e1009425. https://doi.org/10.1371/journal.ppat.1009425

Extracellular DNA (eDNA) is a major constituent of the extracellular matrix of Pseudomonas aeruginosa biofilms and its release is regulated via pseudomonas quinolone signal (PQS) dependent quorum sensing (QS). By screening a P. aeruginosa transposon... Read More about Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1sub-unit.

The impaired quorum sensing response of Pseudomonas aeruginosa MexAB-OprM efflux pump overexpressing mutants is not due to non-physiological efflux of 3-oxo-C12-HSL (2020)
Journal Article
Alcalde‐Rico, M., Olivares‐Pacheco, J., Halliday, N., Cámara, M., & Martínez, J. L. (2020). The impaired quorum sensing response of Pseudomonas aeruginosa MexAB-OprM efflux pump overexpressing mutants is not due to non-physiological efflux of 3-oxo-C12-HSL. Environmental Microbiology, 22(12), 5167-5188. https://doi.org/10.1111/1462-2920.15177

Multidrug (MDR) efflux pumps are ancient and conserved molecular machineries with relevant roles in different aspects of the bacterial physiology, besides antibiotic resistance. In the case of the environmental opportunistic pathogen Pseudomonas aeru... Read More about The impaired quorum sensing response of Pseudomonas aeruginosa MexAB-OprM efflux pump overexpressing mutants is not due to non-physiological efflux of 3-oxo-C12-HSL.

Cross-kingdom signalling regulates spore germination in the moss Physcomitrella patens (2020)
Journal Article
Vesty, E. F., Whitbread, A. L., Needs, S., Tanko, W., Jones, K., Halliday, N., …Coates, J. C. (2020). Cross-kingdom signalling regulates spore germination in the moss Physcomitrella patens. Scientific Reports, 10(1), Article 2614. https://doi.org/10.1038/s41598-020-59467-5

Plants live in close association with microorganisms that can have beneficial or detrimental effects. The activity of bacteria in association with flowering plants has been extensively analysed. Bacteria use quorum-sensing as a way of monitoring thei... Read More about Cross-kingdom signalling regulates spore germination in the moss Physcomitrella patens.

Azospirillum brasilense Az39, a model rhizobacterium with AHL quorum quenching capacity (2019)
Journal Article
Gualpa, J., Lopez, G., Nievas, S., Coniglio, A., Halliday, N., Cámara, M., & Cassán, F. (2019). Azospirillum brasilense Az39, a model rhizobacterium with AHL quorum quenching capacity. Journal of Applied Microbiology, 126(6), 1850-1860. https://doi.org/10.1111/jam.14269

Aims The aim of this research was to analyze the Quorum sensing (QS) and Quorum quenching (QQ) mechanisms based on N‐acyl‐L‐homoserine lactones (AHLs) in A. brasilense Az39, a strain with remarkable capacity to benefit a wide range of crops under... Read More about Azospirillum brasilense Az39, a model rhizobacterium with AHL quorum quenching capacity.

Contribution of the alkylquinolone quorum sensing system to the interaction of Pseudomonas aeruginosa with bronchial epithelial cells. (2018)
Journal Article
Liu, Y.-C., Hussain, F., Negm, O., Pavia, A., Halliday, N., Frédéric Dubern, J.-., …Martínez-Pomares, L. (2018). Contribution of the alkylquinolone quorum sensing system to the interaction of Pseudomonas aeruginosa with bronchial epithelial cells. Frontiers in Microbiology, 9, Article 3018. https://doi.org/10.3389/fmicb.2018.03018

Pseudomonas aeruginosa causes infections in patients with compromised epithelial 32 barrier function. Multiple virulence factors produced by P. aeruginosa are controlled 33 by quorum sensing (QS) via 2-alkyl-4(1H)-quinolone (AQ) signal molecules. Her... Read More about Contribution of the alkylquinolone quorum sensing system to the interaction of Pseudomonas aeruginosa with bronchial epithelial cells..

Model-based drug development in pulmonary delivery: pharmacokinetic analysis of novel drug candidates for treatment of Pseudomonas aeruginosa lung infection (2018)
Journal Article
Sou, T., Kukavica-Ibrulj, I., Soukarieh, F., Halliday, N., Levesque, R. C., Williams, P., …Bergström, C. A. (2019). Model-based drug development in pulmonary delivery: pharmacokinetic analysis of novel drug candidates for treatment of Pseudomonas aeruginosa lung infection. Journal of Pharmaceutical Sciences, 108(1), 630-640. https://doi.org/10.1016/j.xphs.2018.09.017

Antibiotic resistance is a major public health threat worldwide. In particular, about 80% of cystic fibrosis patients have chronic Pseudomonas aeruginosa (PA) lung infection resistant to many current antibiotics. We are therefore developing a novel c... Read More about Model-based drug development in pulmonary delivery: pharmacokinetic analysis of novel drug candidates for treatment of Pseudomonas aeruginosa lung infection.

Identification of FDA-Approved Drugs as Antivirulence Agents Targeting the pqs Quorum-Sensing System of Pseudomonas aeruginosa (2018)
Journal Article
D'Angelo, F., Baldelli, V., Halliday, N., Pantalone, P., Polticelli, F., Fiscarelli, E., …Rampioni, G. (2018). Identification of FDA-Approved Drugs as Antivirulence Agents Targeting the pqs Quorum-Sensing System of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 62(11), Article e01296-18. https://doi.org/10.1128/AAC.01296-18

Copyright © 2018 American Society for Microbiology. All Rights Reserved. The long-term use of antibiotics has led to the emergence of multidrug-resistant bacteria. A promising strategy to combat bacterial infections aims at hampering their adaptabili... Read More about Identification of FDA-Approved Drugs as Antivirulence Agents Targeting the pqs Quorum-Sensing System of Pseudomonas aeruginosa.

Quorum sensing in Pseudomonas savastanoi pv. savastanoi and Erwinia toletana: role in virulence and interspecies interactions in the olive knot (2018)
Journal Article
Caballo-Ponce, E., Meng, X., Uzelac, G., Halliday, N., Cámara, M., Licastro, D., …Venturi, V. (2018). Quorum sensing in Pseudomonas savastanoi pv. savastanoi and Erwinia toletana: role in virulence and interspecies interactions in the olive knot. Applied and Environmental Microbiology, 84(18), 1-18. https://doi.org/10.1128/AEM.00950-18

The olive-knot disease (Olea europea L.) is caused by the bacterium Pseudomonas savastanoi pv. savastanoi (PSV). PSV in the olive-knot undergoes interspecies interactions with the harmless endophyte Erwina toletana (ET); PSV and ET co-localize and fo... Read More about Quorum sensing in Pseudomonas savastanoi pv. savastanoi and Erwinia toletana: role in virulence and interspecies interactions in the olive knot.

In Silico and in Vitro-Guided Identification of Inhibitors of Alkylquinolone-Dependent Quorum Sensing in Pseudomonas aeruginosa (2018)
Journal Article
Soukarieh, F., Vico Oton, E., Dubern, J.-F., Gomes, J., Halliday, N., de Pilar Crespo, M., …Cámara, M. (2018). In Silico and in Vitro-Guided Identification of Inhibitors of Alkylquinolone-Dependent Quorum Sensing in Pseudomonas aeruginosa. Molecules, 23(2), 1-15. https://doi.org/10.3390/molecules23020257

Pseudomonas aeruginosa is a major opportunistic pathogen in cystic fibrosis, wound and nosocomial infections, posing a serious burden to public health, due to its antibiotic resistance. The P. aeruginosa Pseudomonas Quinolone System (pqs) quorum sens... Read More about In Silico and in Vitro-Guided Identification of Inhibitors of Alkylquinolone-Dependent Quorum Sensing in Pseudomonas aeruginosa.

Diagnostic and prognostic significance of systemic alkyl quinolones for P. aeruginosa in cystic fibrosis: a longitudinal study (2016)
Journal Article
Barr, H. L., Halliday, N., Barrett, D. A., Williams, P., Forrester, D. L., Peckham, D., …Fogarty, A. W. (2017). Diagnostic and prognostic significance of systemic alkyl quinolones for P. aeruginosa in cystic fibrosis: a longitudinal study. Journal of Cystic Fibrosis, 16(2), 230-238. https://doi.org/10.1016/j.jcf.2016.10.005

Background Pulmonary P. aeruginosa infection is associated with poor outcomes in cystic fibrosis (CF) and early diagnosis is challenging, particularly in those who are unable to expectorate sputum. Specific P. aeruginosa 2-alkyl-4-quinolones are det... Read More about Diagnostic and prognostic significance of systemic alkyl quinolones for P. aeruginosa in cystic fibrosis: a longitudinal study.

RpoS differentially affects the general stress response and biofilm formation in the endophytic Serratia plymuthica G3 (2015)
Journal Article

The ?S subunit RpoS of RNA polymerase functions as a master regulator of the general stress response in Escherichia coli and related bacteria. RpoS has been reported to modulate biocontrol properties in the rhizobacterium Serratia plymuthica IC1270.... Read More about RpoS differentially affects the general stress response and biofilm formation in the endophytic Serratia plymuthica G3.

Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule (2015)
Journal Article
Soh, E. Y.-C., Chhabra, S. R., Halliday, N., Heeb, S., Müller, C., Birmes, F. S., …Williams, P. (2015). Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule. Environmental Microbiology, 17(11), https://doi.org/10.1111/1462-2920.12857

In Pseudomonas aeruginosa, quorum sensing (QS) regulates the production of secondary metabolites, many of which are antimicrobials that impact on polymicrobial community composition. Consequently, quenching QS modulates the environmental impact of P.... Read More about Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule.

Pseudomonas aeruginosa quorum sensing molecules correlate with clinical status in cystic fibrosis (2015)
Journal Article
Barr, H. L., Halliday, N., Cámara, M., Barrett, D. A., Williams, P., Forrester, D. L., …Fogarty, A. W. (2015). Pseudomonas aeruginosa quorum sensing molecules correlate with clinical status in cystic fibrosis. European Respiratory Journal, 46(4), 1046-1054. https://doi.org/10.1183/09031936.00225214

Copyright © ERS 2015. Pseudomonas aeruginosa produces quorum sensing signal molecules that are potential biomarkers for infection. A prospective study of 60 cystic fibrosis patients with chronic P. aeruginosa, who required intravenous antibiotics for... Read More about Pseudomonas aeruginosa quorum sensing molecules correlate with clinical status in cystic fibrosis.

Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes (2013)
Journal Article
Lui, L. T., Xue, X., Sui, C., Brown, A., Pritchard, D. I., Halliday, N., …Alexander, C. (2013). Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes. Nature Chemistry, 5(12), https://doi.org/10.1038/nchem.1793

Bacteria deploy a range of chemistries to regulate their behaviour and respond to their environment. Quorum sensing is one mean by which bacteria use chemical reactions to modulate pre-infection behaviour such as surface attachment. Polymers that can... Read More about Bacteria clustering by polymers induces the expression of quorum sense controlled phenotypes.