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Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases (2022)
Journal Article
Franco Cairo, J. P., Mandelli, F., Tramontina, R., Cannella, D., Paradisi, A., Ciano, L., Ferreira, M., Liberato, M. V., Brenelli, L., Gonçalves, T., Rodrigues, G. N., Alvarez, T. M., Mofatto, L. S., Carazzolle, M. F., Pradella, J. G., Leme, A. P., Costa-Leonardo, A. M., Oliveira Neto, M., Damasio, A., Davies, G., …Squina, F. (2022). Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases. Green Chemistry, 24(12), 4845-4858. https://doi.org/10.1039/d1gc04519a

Wood-feeding termites effectively degrade plant biomass through enzymatic degradation. Despite their high efficiencies, however, individual glycoside hydrolases isolated from termites and their symbionts exhibit anomalously low effectiveness in ligno... Read More about Oxidative cleavage of polysaccharides by a termite-derived superoxide dismutase boosts the degradation of biomass by glycoside hydrolases.

Activity and substrate specificity of lytic polysaccharide monooxygenases: An ATR FTIR-based sensitive assay tested on a novel species from Pseudomonas putida (2021)
Journal Article
Serra, I., Piccinini, D., Paradisi, A., Ciano, L., Bellei, M., Bortolotti, C. A., Battistuzzi, G., Sola, M., Walton, P. H., & Di Rocco, G. (2022). Activity and substrate specificity of lytic polysaccharide monooxygenases: An ATR FTIR-based sensitive assay tested on a novel species from Pseudomonas putida. Protein Science, 31(3), 591-601. https://doi.org/10.1002/pro.4255

Pseudomonas putida W619 is a soil Gram-negative bacterium commonly used in environmental studies thanks to its ability in degrading many aromatic compounds. Its genome contains several putative carbohydrate-active enzymes such as glycoside hydrolases... Read More about Activity and substrate specificity of lytic polysaccharide monooxygenases: An ATR FTIR-based sensitive assay tested on a novel species from Pseudomonas putida.

Mechanistic basis of substrate–O2 coupling within a chitin-active lytic polysaccharide monooxygenase: An integrated NMR/EPR study (2020)
Journal Article
Courtade, G., Ciano, L., Paradisi, A., Lindley, P. J., Forsberg, Z., Sørlie, M., Wimmer, R., Davies, G. J., Eijsink, V. G. H., Walton, P. H., & Aachmann, F. L. (2020). Mechanistic basis of substrate–O2 coupling within a chitin-active lytic polysaccharide monooxygenase: An integrated NMR/EPR study. Proceedings of the National Academy of Sciences, 117(32), Article 202004277. https://doi.org/10.1073/pnas.2004277117

Lytic polysaccharide monooxygenases (LPMOs) have a unique ability to activate molecular oxygen for subsequent oxidative cleavage of glycosidic bonds. To provide insight into the mode of action of these industrially important enzymes, we have performe... Read More about Mechanistic basis of substrate–O2 coupling within a chitin-active lytic polysaccharide monooxygenase: An integrated NMR/EPR study.

Insights from semi-oriented EPR spectroscopy studies into the interaction of lytic polysaccharide monooxygenases with cellulose (2020)
Journal Article
Ciano, L., Paradisi, A., Hemsworth, G. R., Tovborg, M., Davies, G. J., & Walton, P. H. (2020). Insights from semi-oriented EPR spectroscopy studies into the interaction of lytic polysaccharide monooxygenases with cellulose. Dalton Transactions, 49(11), 3413-3422. https://doi.org/10.1039/c9dt04065j

Probing the detailed interaction between lytic polysaccharide monooxygenases (LPMOs) and their polysaccharide substrates is key to revealing further insights into the mechanism of action of this class of enzymes on recalcitrant biomass. This investig... Read More about Insights from semi-oriented EPR spectroscopy studies into the interaction of lytic polysaccharide monooxygenases with cellulose.

Discovery of a fungal copper radical oxidase with high catalytic efficiency towards 5-hydroxymethylfurfural and benzyl alcohols for bioprocessing (2020)
Journal Article
Mathieu, Y., Offen, W. A., Forget, S. M., Ciano, L., Viborg, A. H., Blagova, E., Henrissat, B., Walton, P. H., Davies, G. J., & Brumer, H. (2020). Discovery of a fungal copper radical oxidase with high catalytic efficiency towards 5-hydroxymethylfurfural and benzyl alcohols for bioprocessing. ACS Catalysis, 10(5), 3042-3058. https://doi.org/10.1021/acscatal.9b04727

Copyright © 2020 American Chemical Society. Alternatives to petroleum-based chemicals are highly sought-after for ongoing efforts to reduce the damaging effects of human activity on the environment. Copper radical oxidases from Auxiliary Activity Fam... Read More about Discovery of a fungal copper radical oxidase with high catalytic efficiency towards 5-hydroxymethylfurfural and benzyl alcohols for bioprocessing.

A fungal family of lytic polysaccharide monooxygenase-like copper proteins (2020)
Journal Article
Labourel, A., Frandsen, K. E. H., Zhang, F., Brouilly, N., Grisel, S., Haon, M., Ciano, L., Ropartz, D., Fanuel, M., Martin, F., Navarro, D., Rosso, M.-N., Tandrup, T., Bissaro, B., Johansen, K. S., Zerva, A., Walton, P. H., Henrissat, B., Leggio, L. L., & Berrin, J.-G. (2020). A fungal family of lytic polysaccharide monooxygenase-like copper proteins. Nature Chemical Biology, 16(3), 345–350. https://doi.org/10.1038/s41589-019-0438-8

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that play a key role in the oxidative degradation of various biopolymers such as cellulose and chitin.
While hunting for new LPMOs, we identified in various fungal lineages... Read More about A fungal family of lytic polysaccharide monooxygenase-like copper proteins.

Formation of a Copper(II)–Tyrosyl Complex at the Active Site of Lytic Polysaccharide Monooxygenases Following Oxidation by H2O2 (2019)
Journal Article
Paradisi, A., Johnston, E. M., Tovborg, M., Nicoll, C. R., Ciano, L., Dowle, A., McMaster, J., Hancock, Y., Davies, G. J., & Walton, P. H. (2019). Formation of a Copper(II)–Tyrosyl Complex at the Active Site of Lytic Polysaccharide Monooxygenases Following Oxidation by H2O2. Journal of the American Chemical Society, 141(46), 18585-18599. https://doi.org/10.1021/jacs.9b09833

Hydrogen peroxide is a cosubstrate for the oxidative cleavage of saccharidic substrates by copper-containing lytic polysaccharide monooxygenases (LPMOs). The rate of reaction of LPMOs with hydrogen peroxide is high, but it is accompanied by rapid in... Read More about Formation of a Copper(II)–Tyrosyl Complex at the Active Site of Lytic Polysaccharide Monooxygenases Following Oxidation by H2O2.

Discovery, activity and characterisation of an AA10 lytic polysaccharide oxygenase from the shipworm symbiont Teredinibacter turnerae (2019)
Journal Article
Fowler, C. A., Sabbadin, F., Ciano, L., Hemsworth, G. R., Elias, L., Bruce, N., McQueen-Mason, S., Davies, G. J., & Walton, P. H. (2019). Discovery, activity and characterisation of an AA10 lytic polysaccharide oxygenase from the shipworm symbiont Teredinibacter turnerae. Biotechnology for Biofuels, 12(1), Article 232. https://doi.org/10.1186/s13068-019-1573-x

An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion (2018)
Journal Article
Sabbadin, F., Hemsworth, G. R., Ciano, L., Henrissat, B., Dupree, P., Tryfona, T., Marques, R. D. S., Sweeney, S. T., Besser, K., Elias, L., Pesante, G., Li, Y., Dowle, A. A., Bates, R., Gomez, L. D., Simister, R., Davies, G. J., Walton, P. H., Bruce, N. C., & McQueen-Mason, S. J. (2018). An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion. Nature Communications, 9, Article 756. https://doi.org/10.1038/s41467-018-03142-x

Thermobia domestica belongs to an ancient group of insects and has a remarkable ability to digest crystalline cellulose without microbial assistance. By investigating the digestive proteome of Thermobia, we have identified over 20 members of an uncha... Read More about An ancient family of lytic polysaccharide monooxygenases with roles in arthropod development and biomass digestion.

Lytic xylan oxidases from wood-decay fungi unlock biomass degradation (2018)
Journal Article
Couturier, M., Ladevèze, S., Sulzenbacher, G., Ciano, L., Fanuel, M., Moreau, C., Villares, A., Cathala, B., Chaspoul, F., Frandsen, K. E., Labourel, A., Herpoël-Gimbert, I., Grisel, S., Haon, M., Lenfant, N., Rogniaux, H., Ropartz, D., Davies, G. J., Rosso, M.-N., Walton, P. H., …Berrin, J.-G. (2018). Lytic xylan oxidases from wood-decay fungi unlock biomass degradation. Nature Chemical Biology, 14(3), 306-310. https://doi.org/10.1038/nchembio.2558

Wood biomass is the most abundant feedstock envisioned for the development of modern biorefineries. However, the cost-effective conversion of this form of biomass into commodity products is limited by its resistance to enzymatic degradation. Here we... Read More about Lytic xylan oxidases from wood-decay fungi unlock biomass degradation.

Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates (2017)
Journal Article
Simmons, T. J., Frandsen, K. E. H., Ciano, L., Tryfona, T., Lenfant, N., Poulsen, J. C., Wilson, L. F. L., Tandrup, T., Tovborg, M., Schnorr, K., Johansen, K. S., Henrissat, B., Walton, P. H., Lo Leggio, L., & Dupree, P. (2017). Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates. Nature Communications, 8(1), Article 1064. https://doi.org/10.1038/s41467-017-01247-3

Lytic polysaccharide monooxygenases (LPMOs) are industrially important copper-dependent enzymes that oxidatively cleave polysaccharides. Here we present a functional and structural characterization of two closely related AA9-family LPMOs from Lentinu... Read More about Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates.

The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases (2016)
Journal Article
Frandsen, K. E. H., Simmons, T. J., Dupree, P., Poulsen, J.-C. N., Hemsworth, G. R., Ciano, L., Johnston, E. M., Tovborg, M., Johansen, K. S., von Freiesleben, P., Marmuse, L., Fort, S., Cottaz, S., Driguez, H., Henrissat, B., Lenfant, N., Tuna, F., Baldansuren, A., Davies, G. J., Lo Leggio, L., & Walton, P. H. (2016). The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases. Nature Chemical Biology, 12(4), 298-303. https://doi.org/10.1038/nchembio.2029

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that oxidatively break down recalcitrant polysaccharides such as cellulose and chitin. Since their discovery, LPMOs have become integral factors in the industrial utilization o... Read More about The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases.

Structure–function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family (2015)
Journal Article
Yin, D., Urresti, S., Lafond, M., Johnston, E. M., Derikvand, F., Ciano, L., Berrin, J.-G., Henrissat, B., Walton, P. H., Davies, G. J., & Brumer, H. (2015). Structure–function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family. Nature Communications, 6(1), Article 10197. https://doi.org/10.1038/ncomms10197

Alcohol oxidases, including carbohydrate oxidases, have a long history of research that has generated fundamental biological understanding and biotechnological applications. Despite a long history of study, the galactose 6-oxidase/glyoxal oxidase fam... Read More about Structure–function characterization reveals new catalytic diversity in the galactose oxidase and glyoxal oxidase family.

An investigation into the cytotoxicity of cis,cis-triaminocyclohexane derivatives (2014)
Thesis
Ciano, L. An investigation into the cytotoxicity of cis,cis-triaminocyclohexane derivatives. (Dissertation). University of York. https://nottingham-repository.worktribe.com/output/23549612

N,N’,N’’-Tris(2-pyridylmethyl)-cis,cis-1,3,5-triaminocyclohexane, tachpyr, has been studied for several years for its potential application in cancer therapy. Several studies have been carried out to understand the mechanism of action of this molecul... Read More about An investigation into the cytotoxicity of cis,cis-triaminocyclohexane derivatives.