A fungal family of lytic polysaccharide monooxygenase-like copper proteins

Labourel, Aurore; Frandsen, Kristian E. H.; Zhang, Feng; Brouilly, Nicolas; Grisel, Sacha; Haon, Mireille; Ciano, Luisa; Ropartz, David; Fanuel, Mathieu; Martin, Francis; Navarro, David; Rosso, Marie-No�lle; Tandrup, Tobias; Bissaro, Bastien; Johansen, Katja S.; Zerva, Anastasia; Walton, Paul H.; Henrissat, Bernard; Leggio, Leila Lo; Berrin, Jean-Guy

Abstract

Lytic polysaccharide monooxygenases (LPMOs) are copper-containing enzymes that play a key role in the oxidative degradation of various biopolymers such as cellulose and chitin.
While hunting for new LPMOs, we identified in various fungal lineages a new family of proteins, defined herein as X325. The X325 three-dimensional structure revealed an overall LPMO fold and a histidine-brace with an additional aspartate ligand to Cu(II). Although LPMO-type activity of X325 members was initially expected, we demonstrated that X325 members do not perform oxidative cleavage of polysaccharides, establishing that X325s are not LPMOs. Investigations of the biological role of X325 in the ectomycorrhizal fungus Laccaria bicolor revealed exposure of the X325 protein at the interface between fungal hyphae and tree rootlet cells. Our results provide insights into a family of copper-containing proteins widespread in the fungal kingdom, which is evolutionarily related to LPMOs but has diverged to biological functions other than polysaccharide degradation.

Citation

Labourel, A., Frandsen, K. E. H., Zhang, F., Brouilly, N., Grisel, S., Haon, M., Ciano, L., Ropartz, D., Fanuel, M., Martin, F., Navarro, D., Rosso, M.-N., Tandrup, T., Bissaro, B., Johansen, K. S., Zerva, A., Walton, P. H., Henrissat, B., Leggio, L. L., & Berrin, J.-G. (2020). A fungal family of lytic polysaccharide monooxygenase-like copper proteins. Nature Chemical Biology, 16(3), 345–350. https://doi.org/10.1038/s41589-019-0438-8

• Journal Article Type: Article
• Acceptance Date: Nov 22, 2019
• Online Publication Date: Jan 13, 2020
• Publication Date: Jan 13, 2020
• Deposit Date: Jan 21, 2020
• Publicly Available Date: Jul 14, 2020
• Journal: Nature Chemical Biology
• Print ISSN: 1552-4450
• Electronic ISSN: 1552-4469
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 3
• Pages: 345–350
• DOI: https://doi.org/10.1038/s41589-019-0438-8
• Keywords: Cell biology; Molecular biology
• Public URL: https://nottingham-repository.worktribe.com/output/3774476
• Publisher URL: https://www.nature.com/articles/s41589-019-0438-8
• Additional Information: Received: 9 January 2019; Accepted: 22 November 2019; First Online: 13 January 2020; : The authors declare no competing interests.