All Outputs (8)

Triblock copolymer micelles enhance solubility, permeability and activity of a quorum sensing inhibitor against Pseudomonas aeruginosa biofilms (2024)
Journal Article
Kasza, K., Soukarieh, F., Romero, M., Hardie, K. R., Gurnani, P., Cámara, M., & Alexander, C. (2024). Triblock copolymer micelles enhance solubility, permeability and activity of a quorum sensing inhibitor against Pseudomonas aeruginosa biofilms. RSC Applied Polymers, 2(3), 444-455. https://doi.org/10.1039/D3LP00208J

Antimicrobial resistance is a threat to public health for which new treatments are urgently required. The capability of bacteria to form biofilms is of particular concern as it enables high bacterial tolerance to conventional therapies by reducing dr... Read More about Triblock copolymer micelles enhance solubility, permeability and activity of a quorum sensing inhibitor against Pseudomonas aeruginosa biofilms.

The biocide triclosan induces (p)ppGpp dependent antibiotic tolerance and alters SarA dependent biofilm structures in Staphylococcus aureus (2023)
Preprint / Working Paper
Walsh, D., Salzer, A., Wolz, C., Aylott, J., & Hardie, K. R. The biocide triclosan induces (p)ppGpp dependent antibiotic tolerance and alters SarA dependent biofilm structures in Staphylococcus aureus

The biocide triclosan is used extensively in both household and hospital settings. The chronic exposure to the biocide occurring in individuals that use triclosan-containing products results in low levels of triclosan present in the human body that h... Read More about The biocide triclosan induces (p)ppGpp dependent antibiotic tolerance and alters SarA dependent biofilm structures in Staphylococcus aureus.

JMM profile: rifampicin: a broad-spectrum antibiotic (2022)
Journal Article
Hardie, K. R., & Fenn, S. J. (2022). JMM profile: rifampicin: a broad-spectrum antibiotic. Journal of Medical Microbiology, 71(8), https://doi.org/10.1099/jmm.0.001566

Rifampicin (also known as rifampin) inhibits RNA synthesis, and is used to treat tuberculosis, leprosy, staphylococcal infections and legionnaires' disease. It can also protect at-risk populations from Haemophilus influenzae type b and Neisseria meni... Read More about JMM profile: rifampicin: a broad-spectrum antibiotic.

Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1sub-unit (2021)
Journal Article
Soh, E. Y.-C., Smith, F., Gimenez, M. R., Yang, L., Vejborg, R. M., Fletcher, M., …Williams, P. (2021). Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1sub-unit. PLoS Pathogens, 17(8), Article e1009425. https://doi.org/10.1371/journal.ppat.1009425

Extracellular DNA (eDNA) is a major constituent of the extracellular matrix of Pseudomonas aeruginosa biofilms and its release is regulated via pseudomonas quinolone signal (PQS) dependent quorum sensing (QS). By screening a P. aeruginosa transposon... Read More about Disruption of the Pseudomonas aeruginosa Tat system perturbs PQS-dependent quorum sensing and biofilm maturation through lack of the Rieske cytochrome bc1sub-unit.

Microfluidic-based Growth and Imaging of Bacterial Biofilms (2019)
Journal Article
Brown, J., Blunk, B., Williams, P., & Hardie, K. (2019). Microfluidic-based Growth and Imaging of Bacterial Biofilms. Bio-protocol, 9(24), Article e3460. https://doi.org/10.21769/BioProtoc.3460

Biofilms are a ubiquitous form of growth for bacteria on surfaces in most environments, natural or manmade. Here we present a protocol using the Bioflux microfluidic system to investigate the organized structure and development of these multicellular... Read More about Microfluidic-based Growth and Imaging of Bacterial Biofilms.

Improving children’s and their visitors’ hand hygiene compliance (2019)
Journal Article
Lary, D., Calvert, A., Nerlich, B., Segal, J., Vaughan, N., Randle, J., & Hardie, K. (2020). Improving children’s and their visitors’ hand hygiene compliance. Journal of Infection Prevention, 21(2), 60-67. https://doi.org/10.1177/1757177419892065

Background: Numerous interventions have tried to improve healthcare workers' hand hygiene compliance, however little attention has been paid to children's and their visitors’ compliance. Aim: To increase children’s and visitors’ compliance using in... Read More about Improving children’s and their visitors’ hand hygiene compliance.

Where bacterial metabolism and virulence intersect (2019)
Journal Article
Hardie, K. (2019). Where bacterial metabolism and virulence intersect. Microbiology Today, 46(2), 58-61

‘We are what we eat’, and so are the bacteria that live on us. If bacteria do not have the building blocks or metabolic pathways and capacity to create their healthy cell, they will be disadvantaged when it comes to establishing an infection.

The role of flagella in Clostridium difficile pathogenesis:comparison between a non-epidemic and an epidemicstrain (2013)
Journal Article
Baban, S. T., Kuehne, S. A., Barketi-Klai, A., Cartman, S. T., Kelly, M. L., Hardie, K. R., …Minton, N. P. (2013). The role of flagella in Clostridium difficile pathogenesis:comparison between a non-epidemic and an epidemicstrain. PLoS ONE, 8(9), Article e73026. https://doi.org/10.1371/journal.pone.0073026

Clostridium difficile is a major cause of healthcare-associated infection and inflicts a considerable financial burden on healthcare systems worldwide. Disease symptoms range from self-limiting diarrhoea to fatal pseudomembranous colitis. Whilst C. d... Read More about The role of flagella in Clostridium difficile pathogenesis:comparison between a non-epidemic and an epidemicstrain.