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Microfluidic-based Growth and Imaging of Bacterial Biofilms

Brown, James; Blunk, Birte; Williams, Paul; Hardie, Kim

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Authors

James Brown

Birte Blunk

PAUL WILLIAMS PAUL.WILLIAMS@NOTTINGHAM.AC.UK
Professor of Molecular Microbiology

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KIM HARDIE KIM.HARDIE@NOTTINGHAM.AC.UK
Professor of Bacterial Pathogenesis



Abstract

Biofilms are a ubiquitous form of growth for bacteria on surfaces in most environments, natural or manmade. Here we present a protocol using the Bioflux microfluidic system to investigate the organized structure and development of these multicellular communities. Microfluidic systems present an opportunity to grow biofilms in a stable, physiologically maintained environment that is readily observable via time-lapse microscopy.

Citation

Brown, J., Blunk, B., Williams, P., & Hardie, K. (2019). Microfluidic-based Growth and Imaging of Bacterial Biofilms. Bio-protocol, 9(24), https://doi.org/10.21769/BioProtoc.3460

Journal Article Type Article
Acceptance Date Nov 14, 2019
Online Publication Date Dec 20, 2019
Publication Date Dec 20, 2019
Deposit Date Jan 30, 2020
Publicly Available Date Mar 29, 2024
Journal Bio-protocol
Publisher Bio-Protocol
Peer Reviewed Peer Reviewed
Volume 9
Issue 24
Article Number e3460
DOI https://doi.org/10.21769/BioProtoc.3460
Public URL https://nottingham-repository.worktribe.com/output/3735185
Publisher URL https://bio-protocol.org/bio101/e3460
Additional Information Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC