All Outputs (45)

First-trimester maternal folate and vitamin B12 concentrations and their associations with first-trimester placental growth: the Rotterdam Periconception Cohort (2025)
Journal Article
van Vliet, M. M., Schoenmakers, S., Willemsen, S. P., Sinclair, K. D., & Steegers-Theunissen, R. P. M. (2025). First-trimester maternal folate and vitamin B12 concentrations and their associations with first-trimester placental growth: the Rotterdam Periconception Cohort. Human Reproduction, https://doi.org/10.1093/humrep/deaf095

Study question: Are maternal folate and vitamin B12 concentrations associated with first-trimester placental growth?

Summary answer: Maternal folate concentrations and commencement of folic acid supplements prior to conception, as compared to foll... Read More about First-trimester maternal folate and vitamin B12 concentrations and their associations with first-trimester placental growth: the Rotterdam Periconception Cohort.

Developmental programming: Sex-specific effects of prenatal exposure to a real-life mixture of environmental chemicals on liver function and transcriptome in sheep (2025)
Journal Article
Thangaraj, S. V., Bellingham, M., Lea, R., Evans, N., Sinclair, K., & Padmanabhan, V. (2025). Developmental programming: Sex-specific effects of prenatal exposure to a real-life mixture of environmental chemicals on liver function and transcriptome in sheep. Environmental Pollution, 367, Article 125630. https://doi.org/10.1016/j.envpol.2025.125630

Humans are chronically exposed to a mixture of environmental chemicals (ECs), many with metabolic and endocrine disrupting potential, contributing to non-communicable disease burden. Understanding the effects of chronic exposure to low-level mixtures... Read More about Developmental programming: Sex-specific effects of prenatal exposure to a real-life mixture of environmental chemicals on liver function and transcriptome in sheep.

Developmental epigenetics: Understanding genetic and sexually dimorphic responses to parental diet and outcomes following assisted reproduction (2024)
Journal Article
Sinclair, K. D. (2024). Developmental epigenetics: Understanding genetic and sexually dimorphic responses to parental diet and outcomes following assisted reproduction. Journal of Dairy Science, https://doi.org/10.3168/jds.2024-25811

The developmental integrity and wellbeing of offspring are influenced by events that occur in utero, particularly around the time of conception. While extraneous factors such as environmental temperature and exposure to environmental chemicals can ea... Read More about Developmental epigenetics: Understanding genetic and sexually dimorphic responses to parental diet and outcomes following assisted reproduction.

Smarter Pregnancy Coaching and Maternal Blood Pressure (2024)
Journal Article
Hojeij, B., Schoenmakers, S., Van Mullem, I., Willemsen, S. P., Dinnyes, A., Sinclair, K. D., Rousian, M., & Steegers-Theunissen, R. P. (2025). Smarter Pregnancy Coaching and Maternal Blood Pressure. American Journal of Preventive Medicine, 68(3), 455-464. https://doi.org/10.1016/j.amepre.2024.11.007

Introduction
Maternal lifestyle behaviors can affect blood pressure with consequences for maternal and offspring health. The aim of this study was to investigate the effectiveness of the Smarter Pregnancy digital lifestyle coaching program on matern... Read More about Smarter Pregnancy Coaching and Maternal Blood Pressure.

Ovarian follicular flushing as a means of increasing oocyte yield and in vitro embryo production in cattle (2024)
Journal Article
Simmons, R. J., Tutt, D. A., Kwong, W. Y., Baroni, J. I., Lim, L. N., Cimpeanu, R., Castrejon-Pita, A. A., Vatish, M., Svensson, P., Piegsa, R., Hagby, U., Sinclair, K. D., Sinclair, K. D., & Georgiou, E. X. (2024). Ovarian follicular flushing as a means of increasing oocyte yield and in vitro embryo production in cattle. Reproduction, Fertility and Development, 36(17), Article RD24125. https://doi.org/10.1071/rd24125

Context The number of developmentally competent cumulus-oocyte complexes (COCs) retrieved during Ovum Pick-Up (OPU) determines success in both bovine and human assisted reproduction. Follicular flushing for COC retrieval is practicsed widely in human... Read More about Ovarian follicular flushing as a means of increasing oocyte yield and in vitro embryo production in cattle.

Developmental programming: preconceptional and gestational exposure of sheep to biosolids on offspring ovarian dynamics (2024)
Journal Article
Halloran, K. M., Zhou, Y., Bellingham, M., Lea, R. G., Evans, N. P., Sinclair, K. D., Smith, P., & Padmanabhan, V. (2025). Developmental programming: preconceptional and gestational exposure of sheep to biosolids on offspring ovarian dynamics. Biology of Reproduction, 112(2), 331-345. https://doi.org/10.1093/biolre/ioae166

Developmental exposure to environmental chemicals perturbs establishment and maintenance of the ovarian reserve across the reproductive lifetime, leading to premature follicle depletion and ovarian aging. Considering humans are exposed to a complex m... Read More about Developmental programming: preconceptional and gestational exposure of sheep to biosolids on offspring ovarian dynamics.

Reproductive and Metabolic Health Following Exposure to Environmental Chemicals: Mechanistic Insights from Mammalian Models (2024)
Journal Article
Bellingham, M., Evans, N. P., Lea, R. G., Padmanabhan, V., & Sinclair, K. D. (2025). Reproductive and Metabolic Health Following Exposure to Environmental Chemicals: Mechanistic Insights from Mammalian Models. Annual Review of Animal Biosciences, 13(1), 411-440. https://doi.org/10.1146/annurev-animal-111523-102259

The decline in human reproductive and metabolic health over the past 50 years is associated with exposure to complex mixtures of anthropogenic environmental chemicals (ECs). Real-life EC exposure has varied over time and differs across geographical l... Read More about Reproductive and Metabolic Health Following Exposure to Environmental Chemicals: Mechanistic Insights from Mammalian Models.

In-utero exposure to real-life environmental chemicals disrupts gene expression within the hypothalamo-pituitary-gonadal axis of prepubertal and adult rams (2024)
Journal Article
Ghasemzadeh Hasankolaei, M., Evans, N. P., Elcombe, C. S., Lea, R. G., Sinclair, K. D., Padmanabhan, V., & Bellingham, M. (2025). In-utero exposure to real-life environmental chemicals disrupts gene expression within the hypothalamo-pituitary-gonadal axis of prepubertal and adult rams. Environmental Research, 264, Article 120303. https://doi.org/10.1016/j.envres.2024.120303

Environmental chemicals (ECs) have been associated with a broad range of disorders and diseases. Daily exposure to various ECs in the environment, or real-life exposure, has raised significant public health concerns. Utilizing the biosolids-treated p... Read More about In-utero exposure to real-life environmental chemicals disrupts gene expression within the hypothalamo-pituitary-gonadal axis of prepubertal and adult rams.

Investigative power of Genomic Informational Field Theory (GIFT) relative to GWAS for genotype-phenotype mapping (2024)
Journal Article
Kyratzi, P., Matika, O., Brassington, A. H., Clare, C. E., Xu, J., Barrett, D. A., Emes, R. D., Archibald, A. L., Paldi, A., Sinclair, K. D., Wattis, J., & Rauch, C. (2024). Investigative power of Genomic Informational Field Theory (GIFT) relative to GWAS for genotype-phenotype mapping. Physiological Genomics, 56(11), 791-806. https://doi.org/10.1152/physiolgenomics.00049.2024

Identifying associations between phenotype and genotype is the fundamental basis of genetic analyses. Inspired by frequentist probability and the work of R.A. Fisher, genome-wide association studies (GWAS) extract information using averages and varia... Read More about Investigative power of Genomic Informational Field Theory (GIFT) relative to GWAS for genotype-phenotype mapping.

Investigative power of Genomic Informational Field Theory (GIFT) relative to GWAS for genotype-phenotype mapping (2024)
Preprint / Working Paper
Kyratzi, P., Matika, O., Brassington, A. H., Connie, C. E., Xu, J., Barrett, D. A., Emes, R. D., Archibald, A. L., Paldi, A., Sinclair, K. D., Wattis, J., & Rauch, C. Investigative power of Genomic Informational Field Theory (GIFT) relative to GWAS for genotype-phenotype mapping

Identifying associations between phenotype and genotype is the fundamental basis of genetic analyses. Inspired by frequentist probability and the work of R.A. Fisher, genome-wide association studies (GWAS) extract information using averages and varia... Read More about Investigative power of Genomic Informational Field Theory (GIFT) relative to GWAS for genotype-phenotype mapping.

Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes (2023)
Journal Article
Tutt, D. A. R., Guven-Ates, G., Kwong, W. Y., Simmons, R., Sang, F., Silvestri, G., Canedo-Ribeiro, C., Handyside, A. H., Labrecque, R., Sirard, M. A., Emes, R. D., Griffin, D. K., & Sinclair, K. D. (2023). Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes. Frontiers in Endocrinology, 14, Article 1280847. https://doi.org/10.3389/fendo.2023.1280847

Background: In vitro maturation (IVM) of germinal vesicle intact oocytes prior to in vitro fertilization (IVF) is practiced widely in animals. In human assisted reproduction it is generally reserved for fertility preservation or where ovarian stimula... Read More about Developmental, cytogenetic and epigenetic consequences of removing complex proteins and adding melatonin during in vitro maturation of bovine oocytes.

Sexually dimorphic impact of preconceptional and gestational exposure to a real-life environmental chemical mixture (biosolids) on offspring growth dynamics and puberty in sheep (2023)
Journal Article
Evans, N. P., Bellingham, M., Elcombe, C. S., Ghasemzadeh-Hasankolaei, M., Lea, R. G., Sinclair, K. D., & Padmanabhan, V. (2023). Sexually dimorphic impact of preconceptional and gestational exposure to a real-life environmental chemical mixture (biosolids) on offspring growth dynamics and puberty in sheep. Environmental Toxicology and Pharmacology, 102, Article 104257. https://doi.org/10.1016/j.etap.2023.104257

Humans are ubiquitously exposed to complex mixtures of environmental chemicals (ECs). This study characterised changes in post-natal and peripubertal growth, and the activation of the reproductive axis, in male and female offspring of sheep exposed t... Read More about Sexually dimorphic impact of preconceptional and gestational exposure to a real-life environmental chemical mixture (biosolids) on offspring growth dynamics and puberty in sheep.

Developmental exposure to a real-life environmental chemical mixture alters testicular transcription factor expression in neonatal and pre-pubertal rams, with morphological changes persisting into adulthood (2023)
Journal Article
Elcombe, C. S., Monteiro, A., Ghasemzadeh-Hasankolaei, M., Padmanabhan, V., Lea, R., Sinclair, K. D., Evans, N. P., & Bellingham, M. (2023). Developmental exposure to a real-life environmental chemical mixture alters testicular transcription factor expression in neonatal and pre-pubertal rams, with morphological changes persisting into adulthood. Environmental Toxicology and Pharmacology, 100, Article 104152. https://doi.org/10.1016/j.etap.2023.104152

Environmental chemical (EC) exposure may be impacting male reproductive health. The translationally relevant biosolids treated pasture (BTP) sheep model was used to investigate gestational low-level EC mixture exposure on the testes of F1 male offspr... Read More about Developmental exposure to a real-life environmental chemical mixture alters testicular transcription factor expression in neonatal and pre-pubertal rams, with morphological changes persisting into adulthood.

Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production (2023)
Journal Article
Simmons, R., Tutt, D. A., Tutt, D. A., Guven-Ates, G., Kwong, W. Y., Labrecque, R., Randi, F., & Sinclair, K. D. (2023). Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production. Theriogenology, 199, 77-85. https://doi.org/10.1016/j.theriogenology.2023.01.003

The in vitro production (IVP) of cattle embryos requires that germinal-vesicle stage oocytes undergo a period of maturation in vitro prior to fertilization and culture to the blastocyst stage. Success of IVP in taurine cattle is enhanced following ov... Read More about Enhanced progesterone support during stimulated cycles of transvaginal follicular aspiration improves bovine in vitro embryo production.

Developmental programming: Preconceptional and gestational exposure of sheep to a real-life environmental chemical mixture alters maternal metabolome in a fetal sex-specific manner (2022)
Journal Article
Thangaraj, S. V., Kachman, M., Halloran, K. M., Sinclair, K. D., Lea, R., Bellingham, M., Evans, N. P., & Padmanabhan, V. (2023). Developmental programming: Preconceptional and gestational exposure of sheep to a real-life environmental chemical mixture alters maternal metabolome in a fetal sex-specific manner. Science of the Total Environment, 864, Article 161054. https://doi.org/10.1016/j.scitotenv.2022.161054

Background
Everyday, humans are exposed to a mixture of environmental chemicals some of which have endocrine and/or metabolism disrupting actions which may contribute to non-communicable diseases. The adverse health impacts of real-world chemical ex... Read More about Developmental programming: Preconceptional and gestational exposure of sheep to a real-life environmental chemical mixture alters maternal metabolome in a fetal sex-specific manner.

Periconceptional biomarkers for maternal obesity: a systematic review (2022)
Journal Article
Hojeij, B., Rousian, M., Sinclair, K. D., Dinnyes, A., Steegers-Theunissen, R. P., & Schoenmakers, S. (2023). Periconceptional biomarkers for maternal obesity: a systematic review. Reviews in Endocrine and Metabolic Disorders, 24(2), 139-175. https://doi.org/10.1007/s11154-022-09762-5

Periconceptional maternal obesity is linked to adverse maternal and neonatal outcomes. Identifying periconceptional biomarkers of pathways affected by maternal obesity can unravel pathophysiologic mechanisms and identify individuals at risk of advers... Read More about Periconceptional biomarkers for maternal obesity: a systematic review.

Developmental exposure to real-life environmental chemical mixture programs a testicular dysgenesis syndrome-like phenotype in prepubertal lambs (2022)
Journal Article
Elcombe, C. S., Monteiro, A., Elcombe, M. R., Ghasemzadeh-Hasankolaei, M., Sinclair, K. D., Lea, R., Padmanabhan, V., Evans, N. P., & Bellingham, M. (2022). Developmental exposure to real-life environmental chemical mixture programs a testicular dysgenesis syndrome-like phenotype in prepubertal lambs. Environmental Toxicology and Pharmacology, 94, Article 103913. https://doi.org/10.1016/j.etap.2022.103913

Current declines in male reproductive health may, in part, be driven by anthropogenic environmental chemical (EC) exposure. Using a biosolids treated pasture (BTP) sheep model, this study examined the effects of gestational exposure to a translationa... Read More about Developmental exposure to real-life environmental chemical mixture programs a testicular dysgenesis syndrome-like phenotype in prepubertal lambs.

Effects of single or serial embryo splitting on the development and morphokinetics of in vitro produced bovine embryos (2022)
Journal Article
Silvestri, G., Turner, K. J., Silcock, J. L., Sinclair, K. D., & Griffin, D. K. (2022). Effects of single or serial embryo splitting on the development and morphokinetics of in vitro produced bovine embryos. The European Zoological Journal, 89(1), 680-689. https://doi.org/10.1080/24750263.2022.2077994

Embryo splitting can be used in cattle in vitro production (IVP) to improve embryo availability and to increase selection intensity. Despite this widespread utility, a comparative investigation of the viability of IVP embryos split at Day 2 (2-cell s... Read More about Effects of single or serial embryo splitting on the development and morphokinetics of in vitro produced bovine embryos.

Maternal obesity during pregnancy leads to derangements in one-carbon metabolism and the gut microbiota: implications for fetal development and offspring wellbeing (2022)
Journal Article
Rubini, E., Schenkelaars, N., Rousian, M., Sinclair, K. D., Wekema, L., Faas, M. M., Steegers-Theunissen, R. P., & Schoenmakers, S. (2022). Maternal obesity during pregnancy leads to derangements in one-carbon metabolism and the gut microbiota: implications for fetal development and offspring wellbeing. American Journal of Obstetrics and Gynecology, 227(3), 392-400. https://doi.org/10.1016/j.ajog.2022.04.013

A healthy diet prior to and during pregnancy is beneficial in acquiring essential B vitamins involved in one-carbon metabolism, and to maintain a healthy gut microbiota. Each play important roles in fetal development, immune-system remodeling and pre... Read More about Maternal obesity during pregnancy leads to derangements in one-carbon metabolism and the gut microbiota: implications for fetal development and offspring wellbeing.

First Trimester Maternal Homocysteine and Embryonic and Fetal Growth: The Rotterdam Periconception Cohort (2022)
Journal Article
Rubini, E., Snoek, K. M., Schoenmakers, S., Willemsen, S. P., Sinclair, K. D., Rousian, M., & Steegers-Theunissen, R. P. (2022). First Trimester Maternal Homocysteine and Embryonic and Fetal Growth: The Rotterdam Periconception Cohort. Nutrients, 14(6), Article 1129. https://doi.org/10.3390/nu14061129

Homocysteine is a marker for derangements in one-carbon metabolism. Elevated homocysteine may represent a causal link between poor maternal nutrition and impaired embryonic and fetal development. We sought to investigate associations between referenc... Read More about First Trimester Maternal Homocysteine and Embryonic and Fetal Growth: The Rotterdam Periconception Cohort.