Time-Profiling Fluorescent Reporters in the Arabidopsis Root

Larrieu, Antoine P.; French, Andrew P.; Pridmore, Tony P.; Bennett, Malcolm J.; Wells, Darren M.

doi:10.1007/978-1-62703-592-7_2

All Outputs (4)

Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements During Root Development and Gravitropism (2015)
Book Chapter
Jacques, E., Wells, D. M., Bennett, M. J., & Vissenberg, K. (2015). Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements During Root Development and Gravitropism. In E. B. Blancaflor (Ed.), Plant Gravitropism; Methods and Protocols (81-89). Springer New York. https://doi.org/10.1007/978-1-4939-2697-8_8

High-resolution imaging of cytoskeletal structures paves the way for standardized methods to quantify cytoskeletal organization. Here we provide a detailed description of the analysis performed to determine the microtubule patterns in gravistimulated... Read More about Imaging and Quantitative Methods for Studying Cytoskeletal Rearrangements During Root Development and Gravitropism.

Quantification of fluorescent reporters in plant cells (2014)
Book Chapter
Pound, M., French, A. P., & Wells, D. M. (2014). Quantification of fluorescent reporters in plant cells. In Plant Cell Expansion: Methods and Protocols (123--131). Springer. https://doi.org/10.1007/978-1-4939-1902-4

Quantification of Fluorescent Reporters in Plant Cells (2014)
Book Chapter
Pound, M., French, A. P., & Wells, D. M. (2015). Quantification of Fluorescent Reporters in Plant Cells. In J. M. Estevez (Ed.), Plant Cell Expansion: Methods and Protocols (123-131). Springer. https://doi.org/10.1007/978-1-4939-1902-4_11

© Springer Science+Business Media New York 2015. Fluorescent reporters are powerful tools for plant research. Many studies require accurate determination of fluorescence intensity and localization. Here, we describe protocols for the quantification o... Read More about Quantification of Fluorescent Reporters in Plant Cells.

Time-Profiling Fluorescent Reporters in the Arabidopsis Root (2013)
Book Chapter
Larrieu, A. P., French, A. P., Pridmore, T. P., Bennett, M. J., & Wells, D. M. (2014). Time-Profiling Fluorescent Reporters in the Arabidopsis Root. In Plant chemical genomics : methods and protocols (11-17). Springer. https://doi.org/10.1007/978-1-62703-592-7_2

Confocal laser scanning microscopy is a useful nondestructive approach for the visualization of fluorescent reporters in planta. Samples are usually placed between a slide and a cover slip which, although suited to single time-point imaging, does not... Read More about Time-Profiling Fluorescent Reporters in the Arabidopsis Root.