Time-Profiling Fluorescent Reporters in the Arabidopsis Root

Larrieu, Antoine P.; French, Andrew P.; Pridmore, Tony P.; Bennett, Malcolm J.; Wells, Darren M.

Abstract

Confocal laser scanning microscopy is a useful nondestructive approach for the visualization of fluorescent reporters in planta. Samples are usually placed between a slide and a cover slip which, although suited to single time-point imaging, does not allow long-term observation. Here, we describe a technique to monitor changes in fluorescence in the Arabidopsis root over a long period of time. Treatment can easily be performed, and this approach is suitable for use in low-throughput chemical screens. We also present a rapid method to analyze fluorescence intensity profiles generated using this protocol. © Springer Science+Business Media New York 2014.

Citation

Larrieu, A. P., French, A. P., Pridmore, T. P., Bennett, M. J., & Wells, D. M. (2014). Time-Profiling Fluorescent Reporters in the Arabidopsis Root. In Plant chemical genomics : methods and protocols (11-17). Springer. https://doi.org/10.1007/978-1-62703-592-7_2

• Online Publication Date: Nov 26, 2013
• Publication Date: 2014
• Deposit Date: Jun 23, 2024
• Publisher: Springer
• Peer Reviewed: Peer Reviewed
• Pages: 11-17
• Series Title: Methods in Molecular Biology
• Series Number: 1056
• Series ISSN: 1940-6029
• Book Title: Plant chemical genomics : methods and protocols
• Chapter Number: 2
• ISBN: 9781627035910
• DOI: https://doi.org/10.1007/978-1-62703-592-7_2
• Public URL: https://nottingham-repository.worktribe.com/output/3092512
• Additional Information: First Online: 26 November 2013