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All Outputs (11)

Characterisation of Listeria monocytogenes isolates from cattle using a bovine caruncular epithelial cell model (2020)
Journal Article
Blanchard, A. M., Billenness, R., Warren, J., Glanvill, A., Roden, W., Drinkall, E., …Tötemeyer, S. (2020). Characterisation of Listeria monocytogenes isolates from cattle using a bovine caruncular epithelial cell model. Heliyon, 6(7), Article e04476. https://doi.org/10.1016/j.heliyon.2020.e04476

© 2020 Listeria monocytogenes is an important foodborne pathogen in human and veterinary health, causing significant morbidity and mortality including abortion. It has a particular tropism for the gravid uterus, however, the route of infection in rep... Read More about Characterisation of Listeria monocytogenes isolates from cattle using a bovine caruncular epithelial cell model.

The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals (2019)
Journal Article
Swift, B. M., Meade, N., Barron, E. S., Bennett, M., Perehenic, T., Hughes, V., …Rees, C. E. (2020). The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals. Microbial Biotechnology, 13(3), 738-746. https://doi.org/10.1111/1751-7915.13518

© 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. Here, we describe the development of a method that exploits bacteriophage D29 as a lysis agent for efficient DNA extraction from lo... Read More about The development and use of Actiphage® to detect viable mycobacteria from bovine tuberculosis and Johne’s disease-infected animals.

A novel high sensitivity bacteriophage-based assay identifies low level Mycobacterium tuberculosis bacteraemia in immunocompetent patients with active and incipient tuberculosis (2019)
Journal Article
Verma, R., Swift, B. M., Handley-Hartill, W., Lee, J. K., Woltmann, G., Rees, C. E., & Haldar, P. (2020). A novel high sensitivity bacteriophage-based assay identifies low level Mycobacterium tuberculosis bacteraemia in immunocompetent patients with active and incipient tuberculosis. Clinical Infectious Diseases, 70(5), 933-936. https://doi.org/10.1093/cid/ciz548

Haematogenous dissemination of M. tuberculosis (Mtb) is critical to pathogenesis of progressive tuberculous infection in animal models. Using a novel phage-based blood assay, we report the first concordant evidence in well-characterised immunocompete... Read More about A novel high sensitivity bacteriophage-based assay identifies low level Mycobacterium tuberculosis bacteraemia in immunocompetent patients with active and incipient tuberculosis.

Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk (2018)
Journal Article
Gerrard, Z. E., Swift, B. M., Botsaris, G., Davidson, R. S., Hutchings, M. R., Huxley, J. N., & Rees, C. E. (2018). Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk. Food Microbiology, 74, 57-63. https://doi.org/10.1016/j.fm.2018.03.004

A survey of retail purchased semi-skimmed pasteurised milk (n = 368) for Mycobacterium avium subspecies paratuberculosis (MAP) was conducted between May 2014 and June 2015 across the midlands of England using the Phage-PCR assay. Overall, 10.3% of th... Read More about Survival of Mycobacterium avium subspecies paratuberculosis in retail pasteurised milk.

Evidence that Listeria innocua modulates its membrane’s stored curvature elastic stress, but not fluidity, through the cell cycle (2017)
Journal Article
Furse, S., Jakubec, M., Rise, F., Williams, H. E., Rees, C., & Halskau, Ø. (2017). Evidence that Listeria innocua modulates its membrane’s stored curvature elastic stress, but not fluidity, through the cell cycle. Scientific Reports, 7, Article 8012. https://doi.org/10.1038/s41598-017-06855-z

This paper reports that the abundances of endogenous cardiolipin and phosphatidylethanolamine halve during elongation of the Gram-positive bacterium Listeria innocua. The lyotropic phase behaviour of model lipid systems that describe these modulation... Read More about Evidence that Listeria innocua modulates its membrane’s stored curvature elastic stress, but not fluidity, through the cell cycle.

Draft genome sequences of Listeria monocytogenes, isolated from fresh leaf vegetables in Owerri City, Nigeria (2017)
Journal Article
Nwaiwu, O., Moura, A., Thouvenot, P., Rees, C., Leclercq, A., & Lecuit, M. (2017). Draft genome sequences of Listeria monocytogenes, isolated from fresh leaf vegetables in Owerri City, Nigeria. Genome Announcements, 5(22), Article e00354. https://doi.org/10.1128/genomea.00354-17

Here, we report the draft genome sequences of three Listeria monocytogenes isolates from fresh leaves collected in Nigeria, belonging to sequence types ST5 and ST155 (sublineages SL5 and SL155, respectively).

Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle (2016)
Journal Article
Swift, B. M., Huxley, J., Plain, K. M., Begg, D. J., de Silva, K., Purdie, A. C., …Rees, C. (2016). Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle. BMC Veterinary Research, 12, Article 115. https://doi.org/10.1186/s12917-016-0728-2

Background

Disseminated infection and bacteraemia is an underreported and under-researched aspect of Johne’s disease. This is mainly due to the time it takes for Mycobacterium avium subsp. paratuberculosis (MAP) to grow and lack of sensitivity of... Read More about Evaluation of the limitations and methods to improve rapid phage-based detection of viable Mycobacterium avium subsp. paratuberculosis in the blood of experimentally infected cattle.

Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA (2016)
Journal Article
Swift, B., Convery, T., & Rees, C. (2016). Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA. Virulence, 7(7), 779-788. https://doi.org/10.1080/21505594.2016.1191729

Bovine tuberculosis is a zoonotic infectious disease caused by Mycobacterium bovis 19 that affects cattle and can cause tuberculosis in a range of wildlife animals. A 20 bacteriophage-based method combined with PCR (phage-PCR) has been recently used... Read More about Evidence of Mycobacterium tuberculosis Complex bacteraemia in intradermal skin test positive cattle detected using phage-RPA.

Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria (2014)
Journal Article
Swift, B., Gerrard, Z., Huxley, J., & Rees, C. (2014). Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria. PLoS ONE, 9(9), Article e106690. https://doi.org/10.1371/journal.pone.0106690

Bacteriophages D29 and TM4 are able to infect a wide range of mycobacteria, including pathogenic and non pathogenic species. Successful phage infection of both fast- and slow-growing mycobacteria can be rapidly detected using the phage amplification... Read More about Factors affecting phage D29 infection: a tool to investigate different growth states of mycobacteria.