Skip to main content

Research Repository

Advanced Search

All Outputs (84)

Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers (2016)
Journal Article
Kakde, D., Powell, L. G., Bansal, K. K., Howdle, S., Irvine, D., Mantovani, G., …Alexander, C. (2016). Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers. Journal of Controlled Release, 244(B), https://doi.org/10.1016/j.jconrel.2016.08.003

Polyesters are extensively used in drug delivery because of their controllable biodegradation properties and perceived favorable cytocompatibility. However, new ester-based materials are continually being sought which can be produced from readily acc... Read More about Synthesis, characterization and evaluation of in vitro toxicity in hepatocytes of linear polyesters with varied aromatic and aliphatic co-monomers.

Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles (2016)
Journal Article
Taresco, V., Suksiriworapong, J., Creasey, R., Burley, J. C., Mantovani, G., Alexander, C., & Garnett, M. (2016). Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles. Journal of Polymer Science Part A: Polymer Chemistry, 54(20), 3267-3278. https://doi.org/10.1002/pola.28215

There is an increasing need to develop bio-compatible polymers with an increased range of different physicochemical properties. Poly(glycerol-adipate) (PGA) is a biocompatible, biodegradable amphiphilic polyester routinely produced from divinyl adipa... Read More about Properties of acyl modified poly(glycerol-adipate) comb-like polymers and their self-assembly into nanoparticles.

Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability (2016)
Journal Article
Leire, E., Amaral, S. P., Louzao, I., Winzer, K., Alexander, C., Fernandez-Megia, E., & Fernandez-Trillo, F. (2016). Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability. Biomaterials Science, 4(6), https://doi.org/10.1039/C6BM00079G

Here, we evaluate how cationic gallic acid-triethylene glycol (GATG) dendrimers interact with bacteria and their potential to develop new antimicrobials. We demonstrate that GATG dendrimers functionalised with primary amines in their periphery can in... Read More about Dendrimer mediated clustering of bacteria: improved aggregation and evaluation of bacterial response and viability.

FDCCS16 molecular simulation of the thermophysical properties and phase behaviour of impure CO2 relevant to CCS (2016)
Journal Article
Cresswell, A. J., Wheatley, R. J., Wilkinson, R. D., & Graham, R. S. (in press). FDCCS16 molecular simulation of the thermophysical properties and phase behaviour of impure CO2 relevant to CCS. Faraday Discussions, 192, https://doi.org/10.1039/C6FD00026F

Impurities from the CCS chain can greatly influence the physical properties of CO2. This has important design, safety and cost implications for the compression, transport and storage of CO2. There is an urgent need to understand and predict the prope... Read More about FDCCS16 molecular simulation of the thermophysical properties and phase behaviour of impure CO2 relevant to CCS.

Influence of polymer size on uptake and cytotoxicity of doxorubicin-loaded DNA–PEG conjugates (2016)
Journal Article
Purdie, L., Alexander, C., Spain, S. G., & Magnusson, J. P. (2016). Influence of polymer size on uptake and cytotoxicity of doxorubicin-loaded DNA–PEG conjugates. Bioconjugate Chemistry, 27(5), https://doi.org/10.1021/acs.bioconjchem.6b00085

Intercalation of drugs into assembled DNA systems offers versatile new mechanisms for controlled drug delivery. However, current systems are becoming increasingly complex, reducing the practicality of large scale production. Here, we demonstrate a mo... Read More about Influence of polymer size on uptake and cytotoxicity of doxorubicin-loaded DNA–PEG conjugates.

Variation in structure and properties of poly(glycerol adipate) via control of chain branching during enzymatic synthesis (2016)
Journal Article
Taresco, V., Creasey, R., Kennon, J., Mantovani, G., Alexander, C., Burley, J. C., & Garnett, M. C. (2016). Variation in structure and properties of poly(glycerol adipate) via control of chain branching during enzymatic synthesis. Polymer, 89, https://doi.org/10.1016/j.polymer.2016.02.036

Poly (glycerol adipate) (PGA) can be produced from divinyl adipate and unprotected glycerol by an enzymatic route to generate a polymer with relatively low molar mass (12 kDa). PGA bears a pendant hydroxyl group which imparts a hydrophilic character... Read More about Variation in structure and properties of poly(glycerol adipate) via control of chain branching during enzymatic synthesis.

Receptor crosslinking: a general method to trigger internalization and lysosomal targeting of therapeutic receptor: ligand complexes (2015)
Journal Article

A major unmet clinical need is a universal method for subcellular targeting of bioactive molecules to lysosomes. Delivery to this organelle enables either degradation of oncogenic receptors that are overexpressed in cancers, or release of prodrugs fr... Read More about Receptor crosslinking: a general method to trigger internalization and lysosomal targeting of therapeutic receptor: ligand complexes.

Systemic in vivo delivery of siRNA to tumours using combination of polyethyleneimine and transferrin–polyethyleneimine conjugates (2015)
Journal Article
Grabowska, A. M., Kircheis, R., Kumari, R., Clarke, P., McKenzie, A., Hughes, J., …Alexander, C. (2015). Systemic in vivo delivery of siRNA to tumours using combination of polyethyleneimine and transferrin–polyethyleneimine conjugates. Biomaterials Science, 3(11), https://doi.org/10.1039/C5BM00101C

Materials for delivery of oligonucleotides need to be simple to produce yet effective in vivo to be considered for clinical applications. Formulations of biomaterials based on combinations of existing demonstrated polymeric gene carriers with targete... Read More about Systemic in vivo delivery of siRNA to tumours using combination of polyethyleneimine and transferrin–polyethyleneimine conjugates.

Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides (2015)
Journal Article
Malakooti, N., Alexander, C., & Alvarez-Lorenzo, C. (2015). Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides. Journal of Pharmaceutical Sciences, 104(10), https://doi.org/10.1002/jps.24537

The aim of this work was to develop drug-soft contact lens combination products suitable for controlled release of antimicrobial peptides on the ocular surface. Incorporation of functional monomers and the application of molecular imprinting techniqu... Read More about Imprinted contact lenses for sustained release of polymyxin B and related antimicrobial peptides.

Cationic polymer mediated bacterial clustering: cell-adhesive properties of homo- and copolymers (2015)
Journal Article
Louzao, I., Sui, C., Winzer, K., Fernandez-Trillo, F., & Alexander, C. (2015). Cationic polymer mediated bacterial clustering: cell-adhesive properties of homo- and copolymers. European Journal of Pharmaceutics and Biopharmaceutics, 95, 47-62. https://doi.org/10.1016/j.ejpb.2015.05.026

New anti-infective materials are needed urgently as alternatives to conventional biocides. It has recently been established that polymer materials designed to bind to the surface of bacteria can induce the formation of cell clusters which enhance the... Read More about Cationic polymer mediated bacterial clustering: cell-adhesive properties of homo- and copolymers.

Multiscale modelling of drug-polymer nanoparticle assembly identifies parameters influencing drug encapsulation efficiency (2015)
Journal Article
Mackenzie, R., Booth, J., Alexander, C., Garnett, M., & Laughton, C. A. (2015). Multiscale modelling of drug-polymer nanoparticle assembly identifies parameters influencing drug encapsulation efficiency. Journal of Chemical Theory and Computation, 11(6), https://doi.org/10.1021/ct501152a

Using a multiscale (dual resolution) approach combining an atomistic (GROMOS96) and coarse-grain (MARTINI) force field, we have been able to simulate the process of drug-polymer nanoparticle assembly by nanoprecipitation from mixed solvents. Here we... Read More about Multiscale modelling of drug-polymer nanoparticle assembly identifies parameters influencing drug encapsulation efficiency.

Triblock copolymer nanovesicles for pH-responsive targeted delivery and controlled release of siRNA to cancer cells (2015)
Journal Article
Gallon, E., Matini, T., Sasso, L., Mantovani, G., Armiñan de Benito, A., Sanchis, J., …Salmaso, S. (2015). Triblock copolymer nanovesicles for pH-responsive targeted delivery and controlled release of siRNA to cancer cells. Biomacromolecules, 16(7), https://doi.org/10.1021/acs.biomac.5b00286

New pH-responsive polymersomes for active anticancer oligonucleotide delivery were prepared from triblock copolymers. The delivery systems were formed by two terminal hydrophilic blocks, PEG and polyglycerolmethacrylate (poly-GMA), and a central weak... Read More about Triblock copolymer nanovesicles for pH-responsive targeted delivery and controlled release of siRNA to cancer cells.

Multifunctional poly[N-(2-hydroxypropyl)methacrylamide] copolymers via postpolymerization modification and sequential thiol–ene chemistry (2015)
Journal Article
Francini, N., Purdie, L., Alexander, C., Mantovani, G., & Spain, S. G. (2015). Multifunctional poly[N-(2-hydroxypropyl)methacrylamide] copolymers via postpolymerization modification and sequential thiol–ene chemistry. Macromolecules, 48(9), https://doi.org/10.1021/acs.macromol.5b00447

Poly[N-(2-hydroxypropyl)methacrylamide] is a promising candidate material for biomedical applications. However, synthesis of functional pHPMA via compolymerization results can lead to variations in monomer composition, molar mass, and dispersity maki... Read More about Multifunctional poly[N-(2-hydroxypropyl)methacrylamide] copolymers via postpolymerization modification and sequential thiol–ene chemistry.

Camptothecin prodrug block copolymer micelles with high drug loading and target specificity (2014)
Journal Article
Khan, A. R., Magnusson, J. P., Watson, S., Grabowska, A. M., Wilkinson, R. W., Alexander, C., & Pritchard, D. (2014). Camptothecin prodrug block copolymer micelles with high drug loading and target specificity. Polymer Chemistry, 5(18), 5320-5329. https://doi.org/10.1039/c4py00369a

The clinical efficacy of cytotoxic drugs in the treatment of cancer is often hampered by poor pharmacodynamics and systemic toxicity. Here, we describe the design and synthesis of a new PEG-based system for the delivery of the cytotoxic camptothecin... Read More about Camptothecin prodrug block copolymer micelles with high drug loading and target specificity.

Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition (2014)
Journal Article
Magnusson, J. P., Alexander, C., Fernández-Trillo, F., Sicilia, G., & Spain, S. G. (2014). Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition. Nanoscale, 6(4), 2368-2374. https://doi.org/10.1039/c3nr04952c

Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA c... Read More about Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition.

Multimodal Polymer Nanoparticles with Combined 19F Magnetic Resonance and Optical Detection for Tunable, Targeted, Multimodal Imaging in Vivo (2014)
Journal Article
Rolfe, B. E., Blakey, I., Squires, O., Peng, H., Boase, N. R., Alexander, C., …Thurecht, K. J. (2014). Multimodal Polymer Nanoparticles with Combined 19F Magnetic Resonance and Optical Detection for Tunable, Targeted, Multimodal Imaging in Vivo. Journal of the American Chemical Society, 136(6), 2413-2419. https://doi.org/10.1021/ja410351h

Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres (2014)
Journal Article
Alexander, C., Ivanov, D. P., WALKER, D., Parker, T. L., GARNETT, M., Ashford, M. B., & Gellert, P. R. (2014). Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres. PLoS ONE, 9(8), Article e103817. https://doi.org/10.1371/journal.pone.0103817

Three-dimensional cell culture has many advantages over monolayer cultures, and spheroids have been hailed as the best current representation of small avascular tumours in vitro. However their adoption in regular screening programs has been hindered... Read More about Multiplexing Spheroid Volume, Resazurin and Acid Phosphatase Viability Assays for High-Throughput Screening of Tumour Spheroids and Stem Cell Neurospheres.