Andrea D. Gon�alves
The effect of protein concentration on the viscosity of a recombinant albumin solution formulation
Gon�alves, Andrea D.; Alexander, Cameron; Roberts, Clive J.; Spain, Sebastian G.; Uddin, Shahid; Allen, Stephanie
Authors
Professor CAMERON ALEXANDER CAMERON.ALEXANDER@NOTTINGHAM.AC.UK
PROFESSOR OF POLYMER THERAPEUTICS
Professor CLIVE ROBERTS CLIVE.ROBERTS@NOTTINGHAM.AC.UK
HEAD OF SCHOOL - LIFE SCIENCES
Sebastian G. Spain
Shahid Uddin
Stephanie Allen
Abstract
The effect of protein concentration on solution viscosity in a commercially available biopharmaceutical formulation of recombinant albumin (rAlbumin) was studied. The level of protein aggregation with concentration and its impact on solution viscosity was investigated. Theoretical models predicting viscosity with concentration were applied to these data, and a model that accounts for multiple protein species in solution provided the best fit. The results highlight the need to account for heterogeneity in the level of aggregation when addressing the increase of viscosity observed at high concentrations of protein solutions, a significant issue for the manufacture and use of protein-based therapeutics.
Citation
Gonçalves, A. D., Alexander, C., Roberts, C. J., Spain, S. G., Uddin, S., & Allen, S. (2016). The effect of protein concentration on the viscosity of a recombinant albumin solution formulation. RSC Advances, 6(18), 15143-15154. https://doi.org/10.1039/c5ra21068b
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 28, 2016 |
Publication Date | Jan 29, 2016 |
Deposit Date | Aug 31, 2018 |
Publicly Available Date | Dec 12, 2018 |
Journal | RSC Advances |
Print ISSN | 2046-2069 |
Electronic ISSN | 2046-2069 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 6 |
Issue | 18 |
Pages | 15143-15154 |
DOI | https://doi.org/10.1039/c5ra21068b |
Public URL | https://nottingham-repository.worktribe.com/output/1116650 |
Publisher URL | https://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C5RA21068B#!divAbstract |
PMID | 00036965 |
Files
The effect of protein concentration on the viscosity of a recombinant albumin solution formulation
(1.4 Mb)
PDF
Andrea Author Version 2016
(1.1 Mb)
PDF
You might also like
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search