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The effect of protein concentration on the viscosity of a recombinant albumin solution formulation

Gon�alves, Andrea D.; Alexander, Cameron; Roberts, Clive J.; Spain, Sebastian G.; Uddin, Shahid; Allen, Stephanie

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Authors

Andrea D. Gon�alves

Sebastian G. Spain

Shahid Uddin

Stephanie Allen



Abstract

The effect of protein concentration on solution viscosity in a commercially available biopharmaceutical formulation of recombinant albumin (rAlbumin) was studied. The level of protein aggregation with concentration and its impact on solution viscosity was investigated. Theoretical models predicting viscosity with concentration were applied to these data, and a model that accounts for multiple protein species in solution provided the best fit. The results highlight the need to account for heterogeneity in the level of aggregation when addressing the increase of viscosity observed at high concentrations of protein solutions, a significant issue for the manufacture and use of protein-based therapeutics.

Citation

Gonçalves, A. D., Alexander, C., Roberts, C. J., Spain, S. G., Uddin, S., & Allen, S. (2016). The effect of protein concentration on the viscosity of a recombinant albumin solution formulation. RSC Advances, 6(18), 15143-15154. https://doi.org/10.1039/c5ra21068b

Journal Article Type Article
Acceptance Date Jan 28, 2016
Publication Date Jan 29, 2016
Deposit Date Aug 31, 2018
Publicly Available Date Dec 12, 2018
Journal RSC Advances
Print ISSN 2046-2069
Electronic ISSN 2046-2069
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 6
Issue 18
Pages 15143-15154
DOI https://doi.org/10.1039/c5ra21068b
Public URL https://nottingham-repository.worktribe.com/output/1116650
Publisher URL https://pubs.rsc.org/en/Content/ArticleLanding/2016/RA/C5RA21068B#!divAbstract
PMID 00036965

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