All Outputs (22)

Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies (2023)
Journal Article

Cerebrospinal fluid (CSF) is a clear, transparent fluid derived from blood plasma that protects the brain and spinal cord against mechanical shock, provides buoyancy, clears metabolic waste and transports extracellular components to remote sites in t... Read More about Recommendations for reproducibility of cerebrospinal fluid extracellular vesicle studies.

Extracellular Vesicles Potentiate Medulloblastoma Metastasis in an EMMPRIN and MMP-2 Dependent Manner (2023)
Journal Article

Extracellular vesicles (EVs) have emerged as pivotal mediators of communication in the tumour microenvironment. More specifically, nanosized extracellular vesicles termed exosomes have been shown to contribute to the establishment of a premetastatic... Read More about Extracellular Vesicles Potentiate Medulloblastoma Metastasis in an EMMPRIN and MMP-2 Dependent Manner.

Drug Resistance in Medulloblastoma Is Driven by YB-1, ABCB1 and a Seven-Gene Drug Signature (2023)
Journal Article

Therapy resistance represents an unmet challenge in the treatment of medulloblastoma. Accordingly, the identification of targets that mark drug-resistant cell populations, or drive the proliferation of resistant cells, may improve treatment strategie... Read More about Drug Resistance in Medulloblastoma Is Driven by YB-1, ABCB1 and a Seven-Gene Drug Signature.

Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging (2023)
Journal Article

The most common malignant brain tumour in children, medulloblastoma (MB), is subdivided into four clinically relevant molecular subgroups, although targeted therapy options informed by understanding of different cellular features are lacking. Here, b... Read More about Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging.

Establishing an In Vitro 3D Spheroid Model to Study Medulloblastoma Drug Response and Tumor Dissemination (2022)
Journal Article

Medulloblastoma is the most common malignant pediatric brain tumor. Current treatment involves surgery, chemotherapy, and craniospinal radiotherapy, and these are associated with a significant reduction in quality of life. Metastatic dissemination at... Read More about Establishing an In Vitro 3D Spheroid Model to Study Medulloblastoma Drug Response and Tumor Dissemination.

Chemosensitization of temozolomide-resistant pediatric diffuse midline glioma using potent nanoencapsulated forms of a N(3)-propargyl analogue (2021)
Journal Article

The lack of clinical response to the alkylating agent temozolomide (TMZ) in pediatric diffuse midline/intrinsic pontine glioma (DIPG) has been associated with O6-methylguanine-DNA-methyltransferase (MGMT) expression and mismatch repair deficiency. He... Read More about Chemosensitization of temozolomide-resistant pediatric diffuse midline glioma using potent nanoencapsulated forms of a N(3)-propargyl analogue.

3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination (2021)
Journal Article

© 2021, The Author(s). Studying medulloblastoma, the most common malignant paediatric brain tumour, requires simple yet realistic in vitro models. In this study, we optimised a robust, reliable, three-dimensional (3D) culture method for medulloblasto... Read More about 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination.

ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma (2021)
Journal Article

Background: Therapeutic intervention in metastatic medulloblastoma is dependent upon elucidating the underlying metastatic mechanism. We investigated whether an epithelialmesenchymal transition (EMT)-like pathway could drive medulloblastoma metastasi... Read More about ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma.

Y-box binding protein-1: A neglected target in pediatric brain tumors? (2020)
Journal Article

© 2020 American Association for Cancer Research. Brain and central nervous system tumors represent the most common childhood solid tumors. Comprising 21% of all pediatric cancers, they remain the leading cause of cancer-related mortality and morbidit... Read More about Y-box binding protein-1: A neglected target in pediatric brain tumors?.

3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome (2020)
Journal Article

© 2020 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland. Medulloblastoma (MB) is the most common malignant brain tumour in children and is subdivided into... Read More about 3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome.

Leptomeningeal malignancy of childhood: sharing learning between childhood leukaemia and brain tumour trials (2020)
Journal Article

Leptomeningeal malignancy complicates childhood cancers, including leukaemias, brain tumours, and solid tumours. In leukaemia, such malignancy is thought to invade leptomeninges via the vascular route. In brain tumours, dissemination from the primary... Read More about Leptomeningeal malignancy of childhood: sharing learning between childhood leukaemia and brain tumour trials.

A role for ABCB1 in prognosis, invasion and drug resistance in ependymoma (2019)
Journal Article

© 2019, The Author(s). Three of the hallmarks of poor prognosis in paediatric ependymoma are drug resistance, local invasion and recurrence. We hypothesised that these hallmarks were due to the presence of a sub-population of cancer stem cells expres... Read More about A role for ABCB1 in prognosis, invasion and drug resistance in ependymoma.

A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells (2018)
Journal Article
Ahmed, E. M., Bandopadhyay, G., Coyle, B., & Grabowska, A. M. (2018). A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells. Cellular Oncology, 41(3), https://doi.org/10.1007/s13402-018-0374-8

Purpose Glioblastoma Multiforme (GBM) is the commonest brain tumour in adults. A population of cells, known as cancer stem cells (CSCs), is thought to mediate chemo/radiotherapy resistance. CD133 is a cell surface marker to identify and isolate... Read More about A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells.

Immunostaining for DNA modifications: computational analysis of confocal images (2017)
Journal Article
Ramsawhook, A., Lewis, L. C., Eleftheriou, M., Abakir, A., Durczak, P. M., Markus, R., …Ruzov, A. (2017). Immunostaining for DNA modifications: computational analysis of confocal images. Journal of Visualized Experiments, Article e56318. https://doi.org/10.3791/56318

For several decades, 5-methylcytosine (5mC) has been thought to be the only DNA modification with a functional significance in metazoans. The discovery of enzymatic oxidation of 5mC to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carb... Read More about Immunostaining for DNA modifications: computational analysis of confocal images.

In vitro models of medulloblastoma: choosing the right tool for the job (2016)
Journal Article
Ivanov, D. P., Coyle, B., Walker, D. A., & Grabowska, A. M. (2016). In vitro models of medulloblastoma: choosing the right tool for the job. Journal of Biotechnology, 236, 10-25. https://doi.org/10.1016/j.jbiotec.2016.07.028

The recently-defined four molecular subgroups of medulloblastoma have required updating of our understanding of in vitro models to include molecular classification and risk stratification features from clinical practice. This review seeks to build a... Read More about In vitro models of medulloblastoma: choosing the right tool for the job.

Long-term exposure to irinotecan reduces cell migration in glioma cells. (2016)
Journal Article
Al-Ghafari, A. B., Punjaruk, W., Storer, L., Carrier, D., Hussein, D., Coyle, B., & Kerr, I. D. (2016). Long-term exposure to irinotecan reduces cell migration in glioma cells. Journal of Neuro-Oncology, 127(3), 455-462. https://doi.org/10.1007/s11060-016-2058-4

In spite of considerable research into the therapies for glioblastoma multiforme this tumour type remains very difficult to treat. As well as having a tendency to be inherently resistant to chemotherapy, glioblastoma multiforme also displays local in... Read More about Long-term exposure to irinotecan reduces cell migration in glioma cells..

Overcoming multiple drug resistance mechanisms in medulloblastoma (2014)
Journal Article
Othman, R., Coyle, B., Kimishi, I., Bradshaw, T. D., Storer, L., Korshunov, A., …Kerr, I. D. (2014). Overcoming multiple drug resistance mechanisms in medulloblastoma. Acta Neuropathologica Communications, 2(1), Article 57. https://doi.org/10.1186/2051-5960-2-57

© 2014 Othman et al.; licensee BioMed Central Ltd. Introduction: Medulloblastoma (MB) is the most common malignant paediatric brain tumour. Recurrence and progression of disease occurs in 15-20% of standard risk and 30-40% of high risk patients. We a... Read More about Overcoming multiple drug resistance mechanisms in medulloblastoma.

CD105 (Endoglin) exerts prognostic effects via its role in the microvascular niche of paediatric high grade glioma (2012)
Journal Article
Smith, S. J., Tilly, H., Ward, J. H., Macarthur, D. C., Lowe, J., Coyle, B., & Grundy, R. G. (2012). CD105 (Endoglin) exerts prognostic effects via its role in the microvascular niche of paediatric high grade glioma. Acta Neuropathologica, 124(1), https://doi.org/10.1007/s00401-012-0952-1

Paediatric high grade glioma (pHGG) (World Health Organisation astrocytoma grades III and IV) remains poor prognosis tumours, with a median survival of only 15 months following diagnosis. Current investigation of anti-angiogenic strategies has focuse... Read More about CD105 (Endoglin) exerts prognostic effects via its role in the microvascular niche of paediatric high grade glioma.

Pediatric brain tumor cancer stem cells: cell cycle dynamics, DNA repair, and etoposide extrusion (2010)
Journal Article
Hussein, D., Punjaruk, W., Storer, L. C. D., Shaw, L., Othman, R. T., Peet, A., …Coyle, B. (2011). Pediatric brain tumor cancer stem cells: cell cycle dynamics, DNA repair, and etoposide extrusion. Neuro-Oncology, 13(1), 70-83. https://doi.org/10.1093/neuonc/noq144

Reliable model systems are needed to elucidate the role cancer stem cells (CSCs) play in pediatric brain tumor drug resistance. The majority of studies to date have focused on clinically distinct adult tumors and restricted tumor types. Here, the CSC... Read More about Pediatric brain tumor cancer stem cells: cell cycle dynamics, DNA repair, and etoposide extrusion.

Histone Deacetylase Inhibition Attenuates Cell Growth with Associated Telomerase Inhibition in High-Grade Childhood Brain Tumor Cells (2010)
Journal Article
Rahman, R., Osteso-Ibanez, T., Hirst, R. A., Levesley, J., Kilday, J., Quinn, S., …Grundy, R. G. (2010). Histone Deacetylase Inhibition Attenuates Cell Growth with Associated Telomerase Inhibition in High-Grade Childhood Brain Tumor Cells. Molecular Cancer Therapeutics, 9(9), 2568-2581. https://doi.org/10.1158/1535-7163.mct-10-0272

Aberrant epigenetic regulation of gene expression contributes to tumor initiation and progression. Studies from a plethora of hematologic and solid tumors support the use of histone deacetylase inhibitors (HDACi) as potent anticancer agents. However,... Read More about Histone Deacetylase Inhibition Attenuates Cell Growth with Associated Telomerase Inhibition in High-Grade Childhood Brain Tumor Cells.