All Outputs (35)

Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA (2018)
Journal Article
Capel, V., Vllasaliu, D., Watts, P., Clarke, P. A., Luxton, D., Grabowska, A. M., …Stolnik, S. (in press). Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA. Drug Delivery, 25(1), https://doi.org/10.1080/10717544.2018.1440668

Despite research efforts full potential of siRNA-based therapeutics has not yet been fully realized due to a need for suitable, effective delivery formulations. Here, we examine a potential of a new class of water-soluble chitosans as siRNA platform... Read More about Water-soluble substituted chitosan derivatives as technology platform for inhalation delivery of siRNA.

A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells (2018)
Journal Article
Ahmed, E. M., Bandopadhyay, G., Coyle, B., & Grabowska, A. M. (2018). A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells. Cellular Oncology, 41(3), https://doi.org/10.1007/s13402-018-0374-8

Purpose Glioblastoma Multiforme (GBM) is the commonest brain tumour in adults. A population of cells, known as cancer stem cells (CSCs), is thought to mediate chemo/radiotherapy resistance. CD133 is a cell surface marker to identify and isolate... Read More about A HIF-independent, CD133-mediated mechanism of cisplatin resistance in glioblastoma cells.

Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells (2018)
Journal Article
Alshehri, A. A., Grabowska, A. M., & Stolnik, S. (in press). Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-22166-3

Design of an efficient delivery system is a generally recognised bottleneck in translation of siRNA technology into clinic. Despite research efforts, cellular processes that determine efficiency of siRNA silencing achieved by different delivery formu... Read More about Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells.

In vitro tissue microarrays for quick and efficient spheroid characterisation (2017)
Journal Article
Ivanov, D. P., & Grabowska, A. M. (2018). In vitro tissue microarrays for quick and efficient spheroid characterisation. Slas Discovery, 23(2), https://doi.org/10.1177/2472555217740576

Three-dimensional in vitro microphysiological cultures, such as spheroids and organoids, promise increased patient relevance and therapeutic predictivity compared to reductionist cell monolayers. However, high-throughput characterisation techniques f... Read More about In vitro tissue microarrays for quick and efficient spheroid characterisation.

Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching (2017)
Journal Article
Austin, M., Elliott, L., Nicolaou, N., Grabowska, A. M., & Hulse, R. P. (2017). Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching. Oncotarget, 8(44), https://doi.org/10.18632/oncotarget.20609

The tumour and neuron interaction has a significant impact upon disease progression and the patients quality of life. In breast cancer patients, it is known that there is an interaction between the tumour microenvironment and the sensory neurons to i... Read More about Breast cancer induced nociceptor aberrant growth and collateral sensory axonal branching.

HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells (2017)
Journal Article
Shah, M., Cardenas, R., Wang, B., Persson, J., Mongan, N. P., Grabowska, A. M., & Allegrucci, C. (2017). HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells. Molecular Cancer, 16(38), https://doi.org/10.1186/s12943-017-0605-z

Background: Homeobox genes are master regulators of cell fate during embryonic development and their expression is altered in cancer. By regulating the balance between cell proliferation and differentiation, they maintain homeostasis of normal tissue... Read More about HOXC8 regulates self-renewal, differentiation and transformation of breast cancer stem cells.

Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D (2017)
Journal Article
Ivanov, D. P., & Grabowska, A. M. (2017). Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D. Scientific Reports, 7(1), Article 41160. https://doi.org/10.1038/srep41160

We describe and share a device, methodology and image analysis algorithms, which allow up to 66 spheroids to be arranged into a gel-based array directly from a culture plate for downstream processing and analysis. Compared to processing individual sa... Read More about Spheroid arrays for high-throughput single-cell analysis of spatial patterns and biomarker expression in 3D.

Individual patient oesophageal cancer 3D models for tailored treatment (2016)
Journal Article
Saunders, J. H., Onion, D., Collier, P., Dorrington, M. S., Argent, R. H., Clarke, P. A., …Grabowska, A. M. (2017). Individual patient oesophageal cancer 3D models for tailored treatment. Oncotarget, 8(15), 24224-24236. https://doi.org/10.18632/oncotarget.12500

Background: A model to predict chemotherapy response would provide a marked clinical benefit, enabling tailored treatment of oesophageal cancer, where less than half of patients respond to the routinely administered chemotherapy. Methods: Cancer cel... Read More about Individual patient oesophageal cancer 3D models for tailored treatment.

Separating chemotherapy-related developmental neurotoxicity from cytotoxicity in monolayer and neurosphere cultures of human fetal brain cells (2016)
Journal Article
Ivanov, D. P., Al-Rubai, A.-J., Grabowska, A. M., & Pratten, M. K. (2016). Separating chemotherapy-related developmental neurotoxicity from cytotoxicity in monolayer and neurosphere cultures of human fetal brain cells. Toxicology in Vitro, 37, 88-96. https://doi.org/10.1016/j.tiv.2016.09.007

Chemotherapy-induced neurotoxicity can reduce the quality of life of patients by affecting their intelligence, senses and mobility. Ten percent of safety-related late-stage clinical failures are due to neurological side effects. Animal models are poo... Read More about Separating chemotherapy-related developmental neurotoxicity from cytotoxicity in monolayer and neurosphere cultures of human fetal brain cells.

Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance (2016)
Journal Article
Secrier, M., Li, X., de Silva, N., Eldridge, M. D., Contino, G., Bornschein, J., …Grimmond, S. M. (2016). Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance. Nature Genetics, 48(10), https://doi.org/10.1038/ng.3659

Esophageal adenocarcinoma (EAC) has a poor outcome, and targeted therapy trials have thus far been disappointing owing to a lack of robust stratification methods. Whole-genome sequencing (WGS) analysis of 129 cases demonstrated that this is a heterog... Read More about Mutational signatures in esophageal adenocarcinoma define etiologically distinct subgroups with therapeutic relevance.

Systemic in vivo delivery of siRNA to tumours using combination of polyethyleneimine and transferrin–polyethyleneimine conjugates (2015)
Journal Article
Grabowska, A. M., Kircheis, R., Kumari, R., Clarke, P., McKenzie, A., Hughes, J., …Alexander, C. (2015). Systemic in vivo delivery of siRNA to tumours using combination of polyethyleneimine and transferrin–polyethyleneimine conjugates. Biomaterials Science, 3(11), https://doi.org/10.1039/C5BM00101C

Materials for delivery of oligonucleotides need to be simple to produce yet effective in vivo to be considered for clinical applications. Formulations of biomaterials based on combinations of existing demonstrated polymeric gene carriers with targete... Read More about Systemic in vivo delivery of siRNA to tumours using combination of polyethyleneimine and transferrin–polyethyleneimine conjugates.

MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers (2014)
Journal Article
Jackson, B. L., Grabowska, A., & Ratan, H. L. (2014). MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers. BMC Cancer, 14(1), 1-10. https://doi.org/10.1186/1471-2407-14-930

Background This non-systematic review article aims to summarise the progress made in understanding the functional consequences of microRNA (miRNA) dysregulation in prostate cancer development, and the identification of potential miRNA targets as ser... Read More about MicroRNA in prostate cancer: functional importance and potential as circulating biomarkers.

Camptothecin prodrug block copolymer micelles with high drug loading and target specificity (2014)
Journal Article
Khan, A. R., Magnusson, J. P., Watson, S., Grabowska, A. M., Wilkinson, R. W., Alexander, C., & Pritchard, D. (2014). Camptothecin prodrug block copolymer micelles with high drug loading and target specificity. Polymer Chemistry, 5(18), 5320-5329. https://doi.org/10.1039/c4py00369a

The clinical efficacy of cytotoxic drugs in the treatment of cancer is often hampered by poor pharmacodynamics and systemic toxicity. Here, we describe the design and synthesis of a new PEG-based system for the delivery of the cytotoxic camptothecin... Read More about Camptothecin prodrug block copolymer micelles with high drug loading and target specificity.

Targeting human apurinic/apyrimidinic endonuclease 1 (APE1) in phosphatase and tensin homolog (PTEN) deficient melanoma cells for personalized therapy (2014)
Journal Article

Phosphatase and tensin homolog (PTEN) loss is associated with genomic instability. APE1 is a key player in DNA base excision repair (BER) and an emerging drug target in cancer. We have developed small molecule inhibitors against APE1 repair nuclease... Read More about Targeting human apurinic/apyrimidinic endonuclease 1 (APE1) in phosphatase and tensin homolog (PTEN) deficient melanoma cells for personalized therapy.

Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries (2005)
Journal Article
Tarr, A. W., Boneham, S. P., Grabowska, A. M., & Ball, J. K. (2005). Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries. Analytical Biochemistry, 339(1), 61-68. https://doi.org/10.1016/j.ab.2004.12.020

Affinity selection of phage display peptide libraries is routinely used for isolating peptides capable of binding a range of molecules, including antibodies and receptors. This process is most successful when the selecting molecule is relatively pure... Read More about Tagged polymerase chain reaction subtractive hybridization for the enrichment of phage display random peptide libraries.