All Outputs (115)

An investigation into IgE-facilitated allergen recognition and presentation by human dendritic cells (2013)
Journal Article

Background: Allergen recognition by dendritic cells (DCs) is a key event in the allergic cascade leading to production of IgE antibodies. C-type lectins, such as the mannose receptor and DC-SIGN, were recently shown to play an important role in the u... Read More about An investigation into IgE-facilitated allergen recognition and presentation by human dendritic cells.

Post-processing of polymer foam tissue scaffolds with high power ultrasound: A route to increased pore interconnectivity, pore size and fluid transport (2013)
Journal Article

The aimof this work is to demonstrate that the structural and fluidic properties of polymer foamtissue scaffolds, post-fabrication but prior to the introduction of cells, can be engineered via exposure to high power ultrasound. Our analysis is suppor... Read More about Post-processing of polymer foam tissue scaffolds with high power ultrasound: A route to increased pore interconnectivity, pore size and fluid transport.

Self-reporting Scaffolds for 3-Dimensional Cell Culture (2013)
Journal Article

Culturing cells in 3D on appropriate scaffolds is thought to better mimic the in vivo microenvironment and increase cell-cell interactions. The resulting 3D cellular construct can often be more relevant to studying the molecular events and cell-cell... Read More about Self-reporting Scaffolds for 3-Dimensional Cell Culture.

Allergen recognition by innate immune cells: critical role of dendritic and epithelial cells (2013)
Journal Article

Allergy is an exacerbated response of the immune system against non-self-proteins called allergens and is typically characterized by biased type-2 T helper cell and deleterious IgE mediated immune responses. The allergic cascade starts with the recog... Read More about Allergen recognition by innate immune cells: critical role of dendritic and epithelial cells.

Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution (2013)
Journal Article

This study aims to investigate interconnectivity and permeability of scCO2-foamed scaffolds and the influence of structural scaffold properties on cell distribution. Supercritical fluid technology was utilized to fabricated scaffolds from 37 kDa, 53... Read More about Interconnectivity and permeability of supercritical fluid-foamed scaffolds and the effect of their structural properties on cell distribution.

Engineering functional epithelium for regenerative medicine and in vitro organ models: A review (2013)
Journal Article

Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing da... Read More about Engineering functional epithelium for regenerative medicine and in vitro organ models: A review.

The development of a 3D immunocompetent model of human skin (2013)
Journal Article

As the first line of defence, skin is regularly exposed to a variety of biological, physical and chemical insults. Therefore, determining the skin sensitization potential of new chemicals is of paramount importance from the safety assessment and regu... Read More about The development of a 3D immunocompetent model of human skin.

The role of lectins in allergic sensitization and allergic disease (2013)
Journal Article

Allergic diseases are a global public health issue affecting millions of persons around the world. However, full understanding of the molecular basis of this group of chronic inflammatory disorders remains rather elusive. Recently, the role of carboh... Read More about The role of lectins in allergic sensitization and allergic disease.

Electrospun PLGA fibre sheets incorporating fluorescent nanosensors: Self-reporting scaffolds for application in tissue engineering (2012)
Journal Article

Ratiometric analyte responsive nanosensors have been incorporated into electrospun poly(lactic-co-glycolic) acid (PLGA) fibres to create self-reporting scaffolds. It has been demonstrated that the self-reporting scaffolds could be utilised to monitor... Read More about Electrospun PLGA fibre sheets incorporating fluorescent nanosensors: Self-reporting scaffolds for application in tissue engineering.

Mammalian innate resistance to highly pathogenic avian influenza H5N1 virus infection is mediated through reduced proinflammation and infectious virus release (2012)
Journal Article

Respiratory epithelial cells and macrophages are the key innate immune cells that play an important role in the pathogenesis of influenza A virus infection. We found that these two cell types from both human and pig showed comparable susceptibilities... Read More about Mammalian innate resistance to highly pathogenic avian influenza H5N1 virus infection is mediated through reduced proinflammation and infectious virus release.

Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications (2012)
Journal Article

The amniotic membrane (AM) is considered as a natural cell culture substrate and has occasionally been exploited in regenerative medicine especially for ocular surface reconstruction and dermal wound healing applications. However, its use is limited... Read More about Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications.

Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease (2012)
Journal Article

Dendritic cells (DCs) are part of the innate immune system with a key role in initiating and modulating T cell mediated immune responses. Coeliac disease is caused by inappropriate activation of such a response leading to small intestinal inflammatio... Read More about Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease.

The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent (2012)
Journal Article

Allergens are initiators of both innate and adaptive immune responses. They are recognised at the site of entry by epithelial and dendritic cells (DCs), both of which activate innate inflammatory circuits that can collectively induce Th2 immune respo... Read More about The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent.

Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite (2012)
Journal Article

Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differenti... Read More about Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.

Biomimetic tissues on a chip for drug discovery (2011)
Journal Article

Developing biologically relevant models of human tissues and organs is an important enabling step for disease modeling and drug discovery. Recent advances in tissue engineering, biomaterials and microfluidics have led to the development of microscale... Read More about Biomimetic tissues on a chip for drug discovery.

Development of a tissue engineered 3D immunocompetent model of the human upper respiratory tract (2011)
Journal Article

Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry (2011)
Journal Article

Uniform cellular distribution is a prerequisite to forming tissue within porous scaffolds, but the seeding process often results in preferential adhesion of cells at the periphery. We develop a vapour phase coating strategy which is readily applicabl... Read More about Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry.

Recognition of the major cat allergen Fel d 1 through the cysteine-rich domain of the mannose receptor determines its allergenicity (2011)
Journal Article

Dendritic cells are professional antigen-presenting cells that are specialized in antigen uptake and presentation. Allergy to cat has increased substantially in recent years and has been shown to be positively associated with asthma.Wehave recently s... Read More about Recognition of the major cat allergen Fel d 1 through the cysteine-rich domain of the mannose receptor determines its allergenicity.

Glycosylation of surface Ig creates a functional bridge between human follicular lymphoma and microenvironmental lectins (2010)
Journal Article

Surface Ig (sIg) of follicular lymphoma (FL) is vital for tumor cell survival. We found previously that the Ig in FL is unusual, because the variable region genes carry sequence motifs for N-glycan addition. These are introduced by somatic mutation a... Read More about Glycosylation of surface Ig creates a functional bridge between human follicular lymphoma and microenvironmental lectins.

Label-free molecular imaging of immunological synapses between dendritic and T cells by Raman micro-spectroscopy (2010)
Journal Article

Confocal Raman micro-spectroscopy (CRMS) was used to measure spectral images of immunological synapse formation between dendritic and T cells without using molecular labels or other invasive procedures. The purpose-built inverted CRMS instrument inte... Read More about Label-free molecular imaging of immunological synapses between dendritic and T cells by Raman micro-spectroscopy.