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Cooperation, quorum sensing, and evolution of virulence in Staphylococcus aureus

Pollitt, Eric J.G.; West, Stuart A.; Crusz, Shanika A.; Burton-Chellew, Maxwell N.; Diggle, Stephen P.

Authors

Eric J.G. Pollitt

Stuart A. West

Shanika A. Crusz

Maxwell N. Burton-Chellew

Stephen P. Diggle steve.diggle@nottingham.ac.uk



Abstract

The virulence and fitness in vivo of the major human pathogen Staphylococcus aureus are associated with a cell-to-cell signaling mechanism known as quorum sensing (QS). QS coordinates the production of virulence factors via the production and sensing of autoinducing peptide (AIP) signal molecules by the agr locus. Here we show, in a wax moth larva virulence model, that (i) QS in S. aureus is a cooperative social trait that provides a benefit to the local population of cells, (ii) agr mutants, which do not pro- duce or respond to QS signal, are able to exploit the benefits provided by the QS of others (“cheat”), allowing them to increase in frequency when in mixed populations with cooperators, (iii) these social interactions between cells determine virulence, with the host mortality rate being negatively correlated to the percentage of agr mutants (“cheats”) in a population, and (iv) a higher within-host relatedness (lower strain diversity) selects for QS and hence higher virulence. Our results provide an explanation for why agr mutants show reduced virulence in animal models but can be isolated from infections of humans. More generally, by providing the first evidence that QS is a cooperative social behavior in a Gram-positive bacterium, our results suggest conver- gent, and potentially widespread, evolution for signaling to coordinate cooperation in bacteria.

Citation

Pollitt, E. J., West, S. A., Crusz, S. A., Burton-Chellew, M. N., & Diggle, S. P. (2014). Cooperation, quorum sensing, and evolution of virulence in Staphylococcus aureus. Infection and Immunity, 82(3), doi:10.1128/IAI.01216-13

Journal Article Type Article
Publication Date Jan 1, 2014
Deposit Date Apr 23, 2014
Publicly Available Date Apr 23, 2014
Journal Infection and Immunity
Print ISSN 0019-9567
Electronic ISSN 1098-5522
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 82
Issue 3
DOI https://doi.org/10.1128/IAI.01216-13
Public URL http://eprints.nottingham.ac.uk/id/eprint/2991
Publisher URL http://iai.asm.org/content/82/3/1045
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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