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Interactions between FGF18 and retinoic acid regulate differentiation of chick embryo limb myoblasts

Mok, Gi Fay; Cardenas, Ryan; Anderton, Helen; Campbell, Keith H.S.; Sweetman, Dylan

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Authors

Gi Fay Mok

Ryan Cardenas

Helen Anderton

Keith H.S. Campbell

Dylan Sweetman



Abstract

During limb development Pax3 positive myoblasts delaminate from the hypaxial dermomyotome of limb level somites and migrate into the limb bud where they form the dorsal and ventral muscle masses. Only then do they begin to differentiate and express markers of myogenic commitment and determination such as Myf5 and MyoD. However the signals regulating this process remain poorly characterised. We show that FGF18, which is expressed in the distal mesenchyme of the limb bud, induces premature expression of both Myf5 and MyoD and that blocking FGF signalling also inhibits endogenous MyoD expression. This expression is mediated by ERK MAP kinase but not PI3K signalling. We also show that retinoic acid (RA) can inhibit the myogenic activity of FGF18 and that blocking RA signalling allows premature induction of MyoD by FGF18 at HH19. We propose a model where interactions between FGF18 in the distal limb and retinoic acid in the proximal limb regulate the timing of myogenic gene expression during limb bud development.

Citation

Mok, G. F., Cardenas, R., Anderton, H., Campbell, K. H., & Sweetman, D. (2014). Interactions between FGF18 and retinoic acid regulate differentiation of chick embryo limb myoblasts. Developmental Biology, 396(2), https://doi.org/10.1016/j.ydbio.2014.10.004

Journal Article Type Article
Publication Date Dec 1, 2014
Deposit Date Jul 3, 2015
Publicly Available Date Jul 3, 2015
Journal Developmental Biology
Print ISSN 0012-1606
Electronic ISSN 0012-1606
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 396
Issue 2
DOI https://doi.org/10.1016/j.ydbio.2014.10.004
Public URL https://nottingham-repository.worktribe.com/output/993669
Publisher URL http://www.sciencedirect.com/science/article/pii/S001216061400520X

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