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A DEMETER-like DNA demethylase protein governs tomato fruit ripening

Liu, Rui E.; How-Kit, Alexandre; Stammitti, Linda; Teyssier, Emeline; Rolin, Dominique; Bertrand, Anne; Halle, Stefanie; Liu, Mingchun; Kong, Junhua; Wu, Chaoqun; Degraeve-Guibault, Charlotte; Chapman, Natalie; Maucourt, Mickael; Hodgman, Charlie; Tost, Jörg; Bouzayen, Mondher; Hong, Yiguo; Seymour, Graham B.; Giovannoni, James; Gallusci, Philippe

Authors

Rui E. Liu

Alexandre How-Kit

Linda Stammitti

Emeline Teyssier

Dominique Rolin

Anne Bertrand

Stefanie Halle

Mingchun Liu

Junhua Kong

Chaoqun Wu

Charlotte Degraeve-Guibault

Natalie Chapman

Mickael Maucourt

Charlie Hodgman

Jörg Tost

Mondher Bouzayen

Yiguo Hong

James Giovannoni

Philippe Gallusci



Abstract

In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DML). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening; an important developmental process unique to plants. RNAi SlDML2 knock-down results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato

Citation

Liu, R. E., How-Kit, A., Stammitti, L., Teyssier, E., Rolin, D., Bertrand, A., …Gallusci, P. (2015). A DEMETER-like DNA demethylase protein governs tomato fruit ripening. Proceedings of the National Academy of Sciences, 112(34), doi:10.1073/pnas.1503362112

Journal Article Type Article
Acceptance Date Jul 8, 2015
Publication Date Aug 1, 2015
Deposit Date Sep 10, 2015
Publicly Available Date Sep 10, 2015
Journal Proceedings of the National Academy of Sciences
Electronic ISSN 1091-6490
Publisher National Academy of Sciences
Peer Reviewed Peer Reviewed
Volume 112
Issue 34
DOI https://doi.org/10.1073/pnas.1503362112
Keywords DNA methylation, fruit ripening, tomato, Demeter like
Public URL http://eprints.nottingham.ac.uk/id/eprint/29891
Related Public URLs http://www.pnas.org/content/112/34/10804.long
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf


PNAS Liu et al 2015 Supplemental dataset 2.pdf (3 Mb)
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Copyright Statement
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PNAS.Liu et al 2015 Supplemental dataset 1.pdf (115 Kb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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