A DEMETER-like DNA demethylase protein governs tomato fruit ripening

Liu, Rui E.; How-Kit, Alexandre; Stammitti, Linda; Teyssier, Emeline; Rolin, Dominique; Bertrand, Anne; Halle, Stefanie; Liu, Mingchun; Kong, Junhua; Wu, Chaoqun; Degraeve-Guibault, Charlotte; Chapman, Natalie; Maucourt, Mickael; Hodgman, Charlie; Tost, J�rg; Bouzayen, Mondher; Hong, Yiguo; Seymour, Graham B.; Giovannoni, James; Gallusci, Philippe

Abstract

In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DML). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripening; an important developmental process unique to plants. RNAi SlDML2 knock-down results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato

Citation

Liu, R. E., How-Kit, A., Stammitti, L., Teyssier, E., Rolin, D., Bertrand, A., Halle, S., Liu, M., Kong, J., Wu, C., Degraeve-Guibault, C., Chapman, N., Maucourt, M., Hodgman, C., Tost, J., Bouzayen, M., Hong, Y., Seymour, G. B., Giovannoni, J., & Gallusci, P. (2015). A DEMETER-like DNA demethylase protein governs tomato fruit ripening. Proceedings of the National Academy of Sciences, 112(34), https://doi.org/10.1073/pnas.1503362112

• Journal Article Type: Article
• Acceptance Date: Jul 8, 2015
• Publication Date: Aug 1, 2015
• Deposit Date: Sep 10, 2015
• Publicly Available Date: Sep 10, 2015
• Journal: Proceedings of the National Academy of Sciences
• Print ISSN: 0027-8424
• Electronic ISSN: 1091-6490
• Publisher: National Academy of Sciences
• Peer Reviewed: Peer Reviewed
• Volume: 112
• Issue: 34
• DOI: https://doi.org/10.1073/pnas.1503362112
• Keywords: DNA methylation, fruit ripening, tomato, Demeter like
• Public URL: https://nottingham-repository.worktribe.com/output/982781
• Related Public URLs: http://www.pnas.org/content/112/34/10804.long
• Contract Date: Sep 10, 2015