Skip to main content

Research Repository

Advanced Search

Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species

Giles, Timothy; Yon, Lisa; Hannant, Duncan; Barrow, Paul; Abu-Median, Abu-Bakr

Authors

Timothy Giles

LISA YON lisa.yon@nottingham.ac.uk
Associate Professor

Duncan Hannant

Paul Barrow

Abu-Bakr Abu-Median



Abstract

The demand for diagnostic tools that allow simultaneous screening of samples for multiple pathogens is increasing because they overcome the limitations of other methods, which can only screen for a single or a few pathogens at a time. Microarrays offer the advantages of being capable to test a large number of samples simultaneously, screening for multiple pathogen types per sample and having comparable sensitivity to existing methods such as PCR. Array design is often considered the most important process in any microarray experiment and can be the deciding factor in the success of a study. There are currently no microarrays for simultaneous detection of rodent-borne pathogens. The aim of this report is to explicate the design, development and evaluation of a microarray platform for use as a screening tool that combines ease of use and rapid identification of a number of rodent-borne pathogens of zoonotic importance. Nucleic acid was amplified by multiplex biotinylation PCR prior to hybridisation onto microarrays. The array sensitivity was comparable to standard PCR, though less sensitive than real-time PCR. The array presented here is a prototype microarray identification system for zoonotic pathogens that can infect rodent species.

Citation

Giles, T., Yon, L., Hannant, D., Barrow, P., & Abu-Median, A. (2015). Development of a DNA-based microarray for the detection of zoonotic pathogens in rodent species. Molecular and Cellular Probes, 29(6), https://doi.org/10.1016/j.mcp.2015.07.005

Journal Article Type Article
Acceptance Date Jul 14, 2015
Online Publication Date Jul 15, 2015
Publication Date Dec 1, 2015
Deposit Date Nov 2, 2016
Publicly Available Date Nov 2, 2016
Journal Molecular and Cellular Probes
Print ISSN 0890-8508
Electronic ISSN 1096-1194
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 29
Issue 6
DOI https://doi.org/10.1016/j.mcp.2015.07.005
Keywords Microarray; Development; Rodent; Zoonoses; ArrayTube; ArrayStrip
Public URL http://eprints.nottingham.ac.uk/id/eprint/38439
Publisher URL http://www.sciencedirect.com/science/article/pii/S0890850815300232
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

Files

Microarray development manuscript text.pdf (264 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





You might also like



Downloadable Citations