Skip to main content

Research Repository

Advanced Search

Quadruple labelled dual oxygen and pH-sensitive ratiometric nanosensors

Chauhan, Veeren M.; Giuntini, Francesca; Aylott, Jonathan W.

Authors

Francesca Giuntini



Abstract

Nanosensors capable of simultaneously measuring dissolved oxygen concentrations from 0 to 100% saturation and pH over the full physiological range, from pH 3.5 to 7.5, that advance the methods towards understanding of key biological gradients, were synthesised. A library of water soluble oxygen-sensitive porphyrins, with three substituted charged functional groups and a chemically flexible carboxylate functional group were spectroscopically analysed to assess their sensitivity to changes in dissolved oxygen concentrations as free species in solution and in suspension as nanoparticle conjugates. A platinum cationic porphyrin was taken forward to fabricate ratiometric oxygen-sensitive nanosensors, using 5-(and-6)-carboxytetramethylrhodamine (TAMRA) as internal standard. In addition, quadruple labelled dual oxygen and pH-sensitive nanosensors were synthesised using the cationic Pt porphyrin, pH-sensitive fluorescein dyes, carboxyfluorescein (FAM) and Oregon Green (OG), in a 1:1 ratio, and TAMRA. We envisage the dual oxygen and pH nanosensors will find broad utility in the characterisation of diverse microenvironments, where there are complex interactions between molecular oxygen and pH.

Citation

Chauhan, V. M., Giuntini, F., & Aylott, J. W. (2016). Quadruple labelled dual oxygen and pH-sensitive ratiometric nanosensors. Sensing and Bio-Sensing Research, 8, https://doi.org/10.1016/j.sbsr.2016.03.007

Journal Article Type Article
Acceptance Date Mar 30, 2016
Online Publication Date Apr 5, 2016
Publication Date May 1, 2016
Deposit Date May 18, 2016
Publicly Available Date May 18, 2016
Journal Sensing and Bio-Sensing Research
Electronic ISSN 2214-1804
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 8
DOI https://doi.org/10.1016/j.sbsr.2016.03.007
Keywords Fluorescent; phosphorescent; nanosensor; oxygen; pH; ratiometric; platinum metalloporphyrin
Public URL http://eprints.nottingham.ac.uk/id/eprint/33336
Publisher URL http://dx.doi.org/10.1016/j.sbsr.2016.03.007
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

1-s2.0-S2214180416300265-main.pdf (2 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





You might also like



Downloadable Citations