Katrin N. Sander
Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels
Sander, Katrin N.; Hay, Daniel P.; Broughton-Pipkin, Fiona; Khan, Raheela N.; Barrett, David A.
Authors
Daniel P. Hay
Fiona Broughton-Pipkin
RAHEELA KHAN RAHEELA.KHAN@NOTTINGHAM.AC.UK
Professor of Cellular Physiology
David A. Barrett
Abstract
© 2018 The analysis of lipids in tough or fibrous biological tissues can be challenging due to difficulties in obtaining a representative sample following homogenisation of the tissue. Furthermore, the choice of normalisation method can have a major effect on the quality of quantitative results. Therefore, a range of mechanical homogenisation techniques and normalisation strategies were evaluated for application to human placental vessels. The findings showed that rotor-stator homogenisation in a suitable solvent and wet weight normalisation were the best combination of procedures for quantitative analysis of lipids in placental blood vessels.
Citation
Sander, K. N., Hay, D. P., Broughton-Pipkin, F., Khan, R. N., & Barrett, D. A. (2018). Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels. Journal of Pharmaceutical and Biomedical Analysis, 160, 297-300. https://doi.org/10.1016/j.jpba.2018.08.012
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 6, 2018 |
Online Publication Date | Aug 7, 2018 |
Publication Date | Oct 25, 2018 |
Deposit Date | Aug 8, 2018 |
Publicly Available Date | Aug 8, 2019 |
Journal | Journal of Pharmaceutical and Biomedical Analysis |
Print ISSN | 0731-7085 |
Electronic ISSN | 1873-264X |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 160 |
Pages | 297-300 |
DOI | https://doi.org/10.1016/j.jpba.2018.08.012 |
Keywords | Eicosanoids; Oxylipins |
Public URL | https://nottingham-repository.worktribe.com/output/973519 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S0731708518311373?via%3Dihub |
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