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Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels

Sander, Katrin N.; Hay, Daniel P.; Broughton-Pipkin, Fiona; Khan, Raheela N.; Barrett, David A.

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Authors

Katrin N. Sander

Daniel P. Hay

Fiona Broughton-Pipkin

RAHEELA KHAN RAHEELA.KHAN@NOTTINGHAM.AC.UK
Professor of Cellular Physiology

David A. Barrett



Abstract

© 2018 The analysis of lipids in tough or fibrous biological tissues can be challenging due to difficulties in obtaining a representative sample following homogenisation of the tissue. Furthermore, the choice of normalisation method can have a major effect on the quality of quantitative results. Therefore, a range of mechanical homogenisation techniques and normalisation strategies were evaluated for application to human placental vessels. The findings showed that rotor-stator homogenisation in a suitable solvent and wet weight normalisation were the best combination of procedures for quantitative analysis of lipids in placental blood vessels.

Citation

Sander, K. N., Hay, D. P., Broughton-Pipkin, F., Khan, R. N., & Barrett, D. A. (2018). Evaluation of extraction and normalisation strategies for the analysis of lipids in placental vessels. Journal of Pharmaceutical and Biomedical Analysis, 160, 297-300. https://doi.org/10.1016/j.jpba.2018.08.012

Journal Article Type Article
Acceptance Date Aug 6, 2018
Online Publication Date Aug 7, 2018
Publication Date Oct 25, 2018
Deposit Date Aug 8, 2018
Publicly Available Date Aug 8, 2019
Journal Journal of Pharmaceutical and Biomedical Analysis
Print ISSN 0731-7085
Electronic ISSN 1873-264X
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 160
Pages 297-300
DOI https://doi.org/10.1016/j.jpba.2018.08.012
Keywords Eicosanoids; Oxylipins
Public URL https://nottingham-repository.worktribe.com/output/973519
Publisher URL https://www.sciencedirect.com/science/article/pii/S0731708518311373?via%3Dihub

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