Jamieson L. Howard
DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase
Howard, Jamieson L.; Killelea, Tom; Hawkins, Michelle; Howard, Jamieson; McGlynn, Peter; Bolt, Edward L.
Authors
TOM KILLELEA TOM.KILLELEA@NOTTINGHAM.AC.UK
Senior Teaching Technician
Michelle Hawkins
Jamieson Howard
Peter McGlynn
ED BOLT ED.BOLT@NOTTINGHAM.AC.UK
Professor of Molecular Biology
Abstract
Cascade complexes underpin E. coli CRISPR-Cas immunity systems by stimulating "adaptation" reactions that update immunity and by initiating "interference" reactions that destroy invader DNA. Recognition of invader DNA in Cascade catalysed R-loops provokes DNA capture and its subsequent integration into CRISPR loci by Cas1 and Cas2. DNA capture processes are unclear but may involve RecG helicase, which stimulates adaptation during its role responding to genome instability. We show that Cascade is a potential source of genome instability because it blocks DNA replication and that RecG helicase alleviates this by dissociating Cascade. This highlights how integrating in vitro CRISPR-Cas interference and adaptation reactions with DNA replication and repair reactions will help to determine precise mechanisms underpinning prokaryotic adaptive immunity.
Citation
Howard, J. L., Killelea, T., Hawkins, M., Howard, J., McGlynn, P., & Bolt, E. L. (2019). DNA replication roadblocks caused by Cascade interference complexes are alleviated by RecG DNA repair helicase. RNA Biology, 16(4), 543-548. https://doi.org/10.1080/15476286.2018.1496773
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 29, 2018 |
Online Publication Date | Aug 10, 2018 |
Publication Date | Apr 3, 2019 |
Deposit Date | Jul 4, 2018 |
Publicly Available Date | Aug 11, 2019 |
Journal | RNA Biology |
Print ISSN | 1547-6286 |
Electronic ISSN | 1555-8584 |
Publisher | Taylor and Francis |
Peer Reviewed | Peer Reviewed |
Volume | 16 |
Issue | 4 |
Pages | 543-548 |
DOI | https://doi.org/10.1080/15476286.2018.1496773 |
Keywords | Cell Biology; Molecular Biology |
Public URL | https://nottingham-repository.worktribe.com/output/942648 |
Publisher URL | https://www.tandfonline.com/doi/abs/10.1080/15476286.2018.1496773?journalCode=krnb20 |
Additional Information | This is an Accepted Manuscript of an article published by Taylor & Francis in RNA Biology on 10 August 2018, available online: http://www.tandfonline.com/10.1080/15476286.2018.1496773 |
Contract Date | Jul 4, 2018 |
Files
Accepted Version.pdf
(726 Kb)
PDF
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