Cristina Carucci
Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support
Carucci, Cristina; Bruen, Larah; Gascón, Victoria; Paradisi, Francesca; Magner, Edmond
Authors
Larah Bruen
Victoria Gascón
Francesca Paradisi
Edmond Magner
Abstract
The use of an in-situ immobilization procedure for the immobilization of hyperhalophilic alcohol dehydrogenase in a metal organic framework material is described. The easy and rapid in situ immobilization process enables retention of activity over a broad range of pH and temperature together with a decrease in the halophilicity of the enzyme. The catalytic activity of the immobilized enzyme was studied in non-aqueous solvent mixtures with the highest retention of activity in aqueous solutions of methanol and acetonitrile. The approach demonstrates that this immobilization method can be extended to hyperhalophilic enzymes with enhancements in activity and stability.
Citation
Carucci, C., Bruen, L., Gascón, V., Paradisi, F., & Magner, E. (in press). Significant enhancement of structural stability of the hyperhalophilic ADH from Haloferax volcanii via entrapment on metal organic framework support. Langmuir, https://doi.org/10.1021/acs.langmuir.8b01037
Journal Article Type | Article |
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Acceptance Date | Jun 19, 2018 |
Online Publication Date | Jun 19, 2018 |
Deposit Date | Jul 13, 2018 |
Publicly Available Date | Jun 20, 2019 |
Journal | Langmuir |
Print ISSN | 0743-7463 |
Electronic ISSN | 1520-5827 |
Publisher | American Chemical Society |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1021/acs.langmuir.8b01037 |
Public URL | https://nottingham-repository.worktribe.com/output/939176 |
Publisher URL | https://pubs.acs.org/doi/abs/10.1021/acs.langmuir.8b01037 |
Contract Date | Jul 13, 2018 |
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