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Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis

Chung, Gary Hong Chun; Domart, Marie-Charlotte; Peddie, Christopher; Mantell, Judith; Mclaverty, Kieran; Arabiotorre, Angela; Hodgson, Lorna; Byrne, Richard D.; Verkade, Paul; Arkill, Kenton P.; Collinson, Lucy M.; Larijani, Banafshé

Acute depletion of diacylglycerol from the Cis-Golgi affects localised nuclear envelope morphology during mitosis Thumbnail


Authors

Gary Hong Chun Chung

Marie-Charlotte Domart

Christopher Peddie

Judith Mantell

Kieran Mclaverty

Angela Arabiotorre

Lorna Hodgson

Richard D. Byrne

Paul Verkade

Lucy M. Collinson

Banafshé Larijani



Abstract

Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis, is a highly regulated process but the detailed mechanism still remains incompletely understood. Previous studies have found isolated diacylglycerol (DAG) containing vesicles are essential for completing the fusion of NE in non-somatic cells. We investigated the impact of DAG depletion from cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a miniSOG-C1-domain tag that localized DAG-enriched vesicles at the perinuclear region, which suggested the existence of NE fusogenic vesicles. We quantified the impact of Golgi-DAG depletion by measuring the in situ NE rim curvature of the re-forming NE. The rim curvature in these cells was significantly reduced compared with controls, which indicated a localized defect in NE morphology. Our novel results demonstrate the significance of the role of DAG from cis-Golgi for the regulation of NE assembly.

Journal Article Type Article
Acceptance Date Jun 12, 2018
Online Publication Date Jun 12, 2018
Publication Date Aug 30, 2018
Deposit Date Jul 17, 2018
Publicly Available Date Jul 17, 2018
Journal Journal of Lipid Research
Print ISSN 0022-2275
Electronic ISSN 1539-7262
Publisher American Society for Biochemistry and Molecular Biology
Peer Reviewed Peer Reviewed
Volume 59
Pages 1402-1413
DOI https://doi.org/10.1194/jlr.M083899
Keywords Cancer; Cell signaling; Diacylglycerol; Electron microscopy; Membranes/fusion; Nuclear envelope formation; Open mitosis
Public URL https://nottingham-repository.worktribe.com/output/937962
Publisher URL http://dx.doi.org/10.1194/jlr.M083899

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