Ravinder Anand-Ivell email@example.com
Development and validation of a mass spectrometry-based assay for quantification of insulin-like factor 3 in human serum
Anand-Ivell, Ravinder; Frederiksen, Hanne; Juul, Anders; Andersson, Anna-Maria; Albrethsen, Jacob; Nordkap, Loa; Kirstine Bang, Anne; Jørgensen, Niels
Anders Juul firstname.lastname@example.org
Jacob Albrethsen Jakob.Christian.Albrethsen@regionh.dk
Anne Kirstine Bang
The circulating level of the peptide hormone insulin-like factor 3 (INSL3) is a promising diagnostic marker reflecting Leydig cell function in the male. Few commercial immunoassays of varying quality exist. Therefore, we decided to develop and validate a precise method for quantification of INSL3 by mass spectrometry.
We developed an assay in which the INSL3 A-chain is released from the INSL3 A-B heterodimer by chemical reduction and alkylation. The alkylated INSL3 A-chain is quantitated by liquid chromatography-tandem mass spectrometry (LC-MS/MS), as substitute for serum INSL3. The method was compared to a validated and sensitive in-house serum INSL3 immunoassay using 97 serum samples from 12 healthy boys during pubertal transition. Adult levels were determined based on sera from 72 adult healthy males aged 18-40 years.
An LC-MS/MS assay with limit of detection and limit of quantification (LOQ) of 0.06 and 0.15 ng/mL, respectively, and intra-assay CVs
|Journal Article Type||Article|
|Publication Date||May 30, 2018|
|Journal||Clinical Chemistry and Laboratory Medicine|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Anand-Ivell, R., Juul, A., & Albrethsen, J. (2018). Development and validation of a mass spectrometry-based assay for quantification of insulin-like factor 3 in human serum. Clinical Chemistry and Laboratory Medicine, 56(11), doi:10.1515/cclm-2018-0171|
|Keywords||assay development; insulin-like factor 3 (INSL3); mass spectrometry|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf|
INSL3 method paper LCMS 2018.pdf
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf
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