Lucky Legbosi Nwidu
In vitro anti-cholinesterase and anti-oxidant activity of three standardised polyherbal products used for memory enhancing in ethnomedicine of South-East Nigeria
Nwidu, Lucky Legbosi; Elmorsy, Ekramy; Carter, Wayne
WAYNE CARTER firstname.lastname@example.org
Background: Polyherbal standardised extracts used in ethnomedicine of Eastern Nigeria for memory improvements were evaluated for anti-cholinesterases and anti-oxidant properties.
Methods: Anti-cholinesterase, anti-oxidant, and total phenolic and flavonoid contents were established using standard procedures.
Results: The three polyherbal extracts exhibited significant concentration dependent acetylcholinesterase (AChE) inhibitory activity (P = 0.001). The highest AChE inhibition was observed with the Neocare Herbal Tea (NHT) with 99.7% (IC50 = 324 ?g/mL); whereas the Herbalin Complex Tea (HCT) and Phytoblis Herbal Tea (PHT) exhibited 73.8% (IC50 = 0.2 ?g/mL) and 60.6% (IC50 = 0.7 ?g/mL) inhibition, respectively, relative to eserine at 100% inhibition (IC50 = 0.9 ?g/mL) at 200 ?g/mL. The order of percentage increase in inhibition of AChE was NHT > HCT > PHT; while the order of decrease in potency was HCT > PHT > NHT.
Radical scavenging activities of HCT, NHT and PHT were 82.13% (IC50 = 0.08 ?g/mL), 77.43% (IC50 = 0.01 ?g/mL) and 76.28% (IC50 = 0.3 ?g/mL), respectively, at 1 mg/mL concentrations. The reducing power revealed a dose-dependent effect, with NHT > PHT > HCT. The order of total phenolics content in the extracts were PHT > HCT > NHT, and for total flavonoids content: PHT > NHT > HCT.
Conclusion: The three polyherbal standardised products possess significant acetylcholinesterase inhibitory activity and secondary metabolites that could collectively contribute to their memory-enhancing effects.
Nwidu, L. L., Elmorsy, E., & Carter, W. (2018). In vitro anti-cholinesterase and anti-oxidant activity of three standardised polyherbal products used for memory enhancing in ethnomedicine of South-East Nigeria. Malaysian Journal of Medical Sciences, 25(2), https://doi.org/10.21315/mjms2018.25.2.4
|Journal Article Type||Article|
|Acceptance Date||Jan 17, 2018|
|Publication Date||Apr 27, 2018|
|Deposit Date||May 8, 2018|
|Publicly Available Date||May 8, 2018|
|Journal||Malaysian Journal of Medical Sciences|
|Peer Reviewed||Peer Reviewed|
|Keywords||Anti-cholinesterases; Anti-oxidants; Memory improvement; Phenolics; Polyherbal|
|Related Public URLs||http://www.mjms.usm.my/default.asp|
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0