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Small non-coding RNA landscape of extracellular vesicles from a post-traumatic model of equine osteoarthritis

Anderson, James R.; Jacobsen, Stine; Walters, Marie; Bundgaard, Louise; Diendorfer, Andreas; Hackl, Matthias; Clarke, Emily J.; James, Victoria; Peffers, Mandy J.

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James R. Anderson

Stine Jacobsen

Marie Walters

Louise Bundgaard

Andreas Diendorfer

Matthias Hackl

Emily J. Clarke

Mandy J. Peffers


Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that the small non-coding RNA expression pattern in synovial fluid and plasma would change during progression of experimental osteoarthritis. In this study, we conducted small RNA sequencing to provide a comprehensive overview of the temporal expression profiles of small non-coding transcripts carried by extracellular vesicles derived from plasma and synovial fluid for the first time in a posttraumatic model of equine osteoarthritis. Additionally, we characterised synovial fluid and plasma-derived extracellular vesicles with respect to quantity, size, and surface markers. The different temporal expressions of seven microRNAs in plasma and synovial fluid-derived extracellular vesicles, eca-miR-451, eca-miR-25, eca-miR-215, eca-miR-92a, eca-miR-let-7c, eca-miR-486-5p, and eca-miR-23a, and four snoRNAs, U3, snord15, snord46, and snord58, represent potential biomarkers for early osteoarthritis. Bioinformatics analysis of the differentially expressed microRNAs in synovial fluid highlighted that in early osteoarthritis these related to the inhibition of cell cycle, cell cycle progression, DNA damage and cell proliferation as well as increased cell viability and differentiation of stem cells. Plasma and synovial fluid-derived extracellular vesicle small non-coding signatures have been established for the first time in a temporal model of osteoarthritis. These could serve as novel biomarkers for evaluation of osteoarthritis progression or act as potential therapeutic targets.

Journal Article Type Article
Acceptance Date Jul 18, 2022
Online Publication Date Aug 8, 2022
Publication Date Aug 8, 2022
Deposit Date Jul 18, 2022
Publicly Available Date Aug 8, 2022
Journal Frontiers in Veterinary Science
Electronic ISSN 2297-1769
Publisher Frontiers Media SA
Peer Reviewed Peer Reviewed
Volume 9
Article Number 901269
Keywords Veterinary Science, osteoarthritis, extracellular vesicles, small non-coding RNA, synovial fluid, plasma
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