Variation in grain Zn concentration, and the grain ionome, in field-grown Indian wheat

Khokhar, Jaswant Singh; Sareen, Sindhu; Tyagi, Bhudeva Singh; Singh, Gyanendra; Wilson, Lolita; King, Ian P.; Young, Scott D.; Broadley, Martin R.

Abstract

Wheat is an important dietary source of zinc (Zn) and other mineral elements in many countries. Dietary Zn deficiency is widespread, especially in developing countries, and breeding (genetic biofortification) through the HarvestPlus programme has recently started to deliver new wheat varieties to help alleviate this problem in South Asia. To better understand the potential of wheat to alleviate dietary Zn deficiency, this study aimed to characterise the baseline effects of genotype (G), site (E), and genotype by site interactions (GxE) on grain Zn concentration under a wide range of soil conditions in India. Field experiments were conducted on a diverse panel of 36 Indian-adapted wheat genotypes, grown on a range of soil types (pH range 4.5–9.5), in 2013–14 (five sites) and 2014–15 (six sites). Grain samples were analysed using inductively coupled plasma-mass spectrometry (ICP-MS). The mean grain Zn concentration of the genotypes ranged from 24.9–34.8 mg kg-1, averaged across site and year. Genotype and site effects were associated with 10% and 6% of the overall variation in grain Zn concentration, respectively. Whilst G x E interaction effects were evident across the panel, some genotypes had consistent rankings between sites and years. Grain Zn concentration correlated positively with grain concentrations of iron (Fe), sulphur (S), and eight other elements, but did not correlate negatively with grain yield, i.e. no yield dilution was observed. Despite a relatively small contribution of genotype to the overall variation in grain Zn concentration, due to experiments being conducted across many contrasting sites and two years, our data are consistent with reports that biofortifying wheat through breeding is likely to be effective at scale given that some genotypes performed consistently across diverse soil types. Notably, all soils in this study were probably Zn deficient and interactions between wheat genotypes and soil Zn availability/management (e.g. the use of Zn-containing fertilisers) need to be better-understood to improve Zn supply in food systems.

Citation

Khokhar, J. S., Sareen, S., Tyagi, B. S., Singh, G., Wilson, L., King, I. P., Young, S. D., & Broadley, M. R. (2018). Variation in grain Zn concentration, and the grain ionome, in field-grown Indian wheat. PLoS ONE, 13(1), Article e0192026. https://doi.org/10.1371/journal.pone.0192026

• Journal Article Type: Article
• Acceptance Date: Jan 16, 2018
• Publication Date: Jan 30, 2018
• Deposit Date: Feb 5, 2018
• Publicly Available Date: Feb 5, 2018
• Journal: PLoS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Peer Reviewed: Peer Reviewed
• Volume: 13
• Issue: 1
• Article Number: e0192026
• DOI: https://doi.org/10.1371/journal.pone.0192026
• Public URL: https://nottingham-repository.worktribe.com/output/908189
• Publisher URL: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0192026
• Contract Date: Feb 5, 2018